Catfish represent 12% of teleost or 6.3% of all vertebrate species, and are of enormous economic value. Here we report a high-quality reference genome sequence of channel catfish (Ictalurus punctatus), the major aquaculture species in the US. The reference genome sequence was validated by genetic mapping of 54,000 SNPs, and annotated with 26,661 predicted protein-coding genes. Through comparative analysis of genomes and transcriptomes of scaled and scaleless fish and scale regeneration experiments, we address the genomic basis for the most striking physical characteristic of catfish, the evolutionary loss of scales and provide evidence that lack of secretory calcium-binding phosphoproteins accounts for the evolutionary loss of scales in catfish. The channel catfish reference genome sequence, along with two additional genome sequences and transcriptomes of scaled catfishes, provide crucial resources for evolutionary and biological studies. This work also demonstrates the power of comparative subtraction of candidate genes for traits of structural significance.

中文翻译：

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channel鱼的基因组序列提供了对硬骨鱼鳞片形成进化的见解。

fish鱼占硬骨鱼类的12％，占所有脊椎动物的6.3％，具有巨大的经济价值。在这里，我们报告了通道cat鱼（Ictalurus punctatus）（美国主要的水产养殖种类）的高质量参考基因组序列。参考基因组序列通过54,000个SNP的遗传图谱验证，并用26,661个预测的蛋白质编码基因进行注释。通过对无鳞鱼和无鳞鱼的基因组和转录组的比较分析以及鳞片再生实验，我们研究了fish鱼最引人注目的物理特征的基因组基础，鳞片的进化损失，并提供了缺乏分泌性钙结合磷蛋白的证据。 cat鱼鳞片的进化损失。channel鱼参考基因组序列，以及两个额外的鳞翅目genome鱼的基因组序列和转录组，为进化和生物学研究提供了至关重要的资源。这项工作还证明了结构结构性状的候选基因的比较减法的能力。