Sustainable production of oleochemicals requires establishment of cell factory platform strains. The yeast Saccharomyces cerevisiae is an attractive cell factory as new strains can be rapidly implemented into existing infrastructures such as bioethanol production plants. Here we show high-level production of free fatty acids (FFAs) in a yeast cell factory, and the production of alkanes and fatty alcohols from its descendants. The engineered strain produces up to 10.4 g l(-1) of FFAs, which is the highest reported titre to date. Furthermore, through screening of specific pathway enzymes, endogenous alcohol dehydrogenases and aldehyde reductases, we reconstruct efficient pathways for conversion of fatty acids to alkanes (0.8 mg l(-1)) and fatty alcohols (1.5 g l(-1)), to our knowledge the highest titres reported in S. cerevisiae. This should facilitate the construction of yeast cell factories for production of fatty acids derived products and even aldehyde-derived chemicals of high value.

中文翻译：

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合成酵母细胞工厂生产脂肪酸衍生的油脂化工产品和生物燃料。

油脂化学品的可持续生产需要建立细胞工厂平台菌株。酵母酿酒酵母是一家有吸引力的细胞工厂，因为可以将新菌株快速实施到现有基础设施中，例如生物乙醇生产厂。在这里，我们显示了酵母细胞工厂中大量生产的游离脂肪酸（FFA），以及其后代生产的烷烃和脂肪醇。该工程菌株可产生高达10.4 g l（-1）的FFA，这是迄今为止报道的最高滴度。此外，通过筛选特定途径的酶，内源性酒精脱氢酶和醛还原酶，我们重建了将脂肪酸转化为烷烃（0.8 mg l（-1））和脂肪醇（1.5 gl（-1））的有效途径。了解酿酒酵母中最高的滴度。