Tissue Adhesive Catechol‐Modified Hyaluronic Acid Hydrogel for Effective, Minimally Invasive Cell Therapy,Advanced Functional Materials

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Tissue Adhesive Catechol‐Modified Hyaluronic Acid Hydrogel for Effective, Minimally Invasive Cell Therapy
Advanced Functional Materials ( IF 18.5 ) Pub Date : 2015-05-15 , DOI: 10.1002/adfm.201500006
Jisoo Shin ₁ , Jung Seung Lee ₁ , Changhyun Lee ₁ , Hyun-Ji Park ₁ , Kisuk Yang ₁ , Yoonhee Jin ₁ , Ji Hyun Ryu ₂ , Ki Sung Hong ₃ , Sung-Hwan Moon ₃ , Hyung-Min Chung ₃ , Hee Seok Yang ₄ , Soong Ho Um ₅ , Jong-Won Oh ₁ , Dong-Ik Kim ₆ , Haeshin Lee ₂ , Seung-Woo Cho _{1,

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Affiliation

Current hyaluronic acid (HA) hydrogel systems often cause cytotoxicity to encapsulated cells and lack the adhesive property required for effective localization of transplanted cells in vivo. In addition, the injection of hydrogel into certain organs (e.g., liver, heart) induces tissue damage and hemorrhage. In this study, we describe a bioinspired, tissue‐adhesive hydrogel that overcomes the limitations of current HA hydrogels through its improved biocompatibility and potential for minimally invasive cell transplantation. HA functionalized with an adhesive catecholamine motif of mussel foot protein forms HA‐catechol (HA‐CA) hydrogel via oxidative crosslinking. HA‐CA hydrogel increases viability, reduces apoptosis, and enhances the function of two types of cells (human adipose‐derived stem cells and hepatocytes) compared with a typical HA hydrogel crosslinked by photopolymerization. Due to the strong tissue adhesiveness of the HA‐CA hydrogel, cells are easily and efficiently transplanted onto various tissues (e.g., liver and heart) without the need for injection. Stem cell therapy using the HA‐CA hydrogel increases angiogenesis in vivo, leading to improved treatment of ischemic diseases. HA‐CA hydrogel also improved hepatic functions of transplanted hepatocytes in vivo. Thus, this bioinspired, tissue‐adhesive HA hydrogel can enhance the efficacy of minimally invasive cell therapy.

中文翻译：

组织邻苯二酚修饰的透明质酸水凝胶可实现有效，微创的细胞治疗

当前的透明质酸（HA）水凝胶系统通常对包封的细胞造成细胞毒性，并且缺乏体内有效定位移植细胞所需的粘附特性。另外，将水凝胶注入某些器官（例如，肝，心脏）会引起组织损伤和出血。在这项研究中，我们描述了一种受生物启发的组织粘附水凝胶，它通过改善的生物相容性和微创细胞移植的潜力克服了目前HA水凝胶的局限性。用贻贝足蛋白的粘附性儿茶酚胺基序功能化的HA通过氧化交联形成HA-儿茶酚（HA-CA）水凝胶。HA‐CA水凝胶可提高活力，减少细胞凋亡，与通过光聚合作用交联的典型HA水凝胶相比，可增强两种类型的细胞（人类脂肪干细胞和肝细胞）的功能。由于HA‐CA水凝胶具有很强的组织粘附性，因此无需注射即可将细胞轻松有效地移植到各种组织（例如肝脏和心脏）上。使用HA-CA水凝胶的干细胞疗法可增加体内血管生成，从而改善缺血性疾病的治疗。HA-CA水凝胶还可以改善体内移植肝细胞的肝功能。因此，这种受生物启发的组织粘附性HA水凝胶可以增强微创细胞治疗的功效。肝脏和心脏），无需注射。使用HA-CA水凝胶的干细胞疗法可增加体内血管生成，从而改善缺血性疾病的治疗。HA-CA水凝胶还可以改善体内移植肝细胞的肝功能。因此，这种受生物启发的组织粘附性HA水凝胶可以增强微创细胞治疗的功效。肝脏和心脏），无需注射。使用HA-CA水凝胶的干细胞疗法可增加体内血管生成，从而改善缺血性疾病的治疗。HA‐CA水凝胶还可以改善体内移植肝细胞的肝功能。因此，这种受生物启发的组织粘附性HA水凝胶可以增强微创细胞治疗的功效。

更新日期：2015-05-15

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