Most traditional methods for pathogen detection, such as the ELISA and PCR, can only detect a single target pathogen at any one time. Here, an antibody array assay system has been developed to detect multiple pathogens in plants at the same time. The system provides a rapid, visual and cheap strategy that combines the pore plate integrated antibody array with a classical double antibody sandwich method. After incubating the array with samples, reaction signals are amplified by specific detection antibodies linked with alkaline phosphatase. All the assays can be completed within 6 hours. Furthermore, the results can be directly examined by the naked eye or by scanning with a flat bet scanner for quantitative analysis. With almost the same sensitivity as the ELISA, the array can detect 10 pathogens in one well simultaneously. This array system is used to screen 24 seed samples (240 detection items in all) and shows a high consistency (240/240) with results from the ELISA, indicating that this antibody array assay system could be used as a rapid method to simultaneously analyse at least ten pathogens, not only for lab research but also for practical applications.

中文翻译：

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一种简单，快速且直观的抗体阵列，可同时检测多种植物病原体†

大多数传统的病原体检测方法（例如ELISA和PCR）只能在任何一次检测到单个目标病原体。在此，已经开发了抗体阵列测定系统以同时检测植物中的多种病原体。该系统提供了一种快速，可视和廉价的策略，该方法将孔板集成抗体阵列与经典的双抗体夹心法相结合。用样品孵育阵列后，反应信号通过与碱性磷酸酶连接的特异性检测抗体进行扩增。所有测定均可在6小时内完成。此外，可以直接用肉眼检查结果，也可以用平板赌注扫描仪进行扫描以进行定量分析。该阵列具有与ELISA几乎相同的灵敏度，可以在一口井中同时检测10种病原体。