Enzyme-Based Test Strips for Visual or Photographic Detection and Quantitation of Gaseous Sulfur Mustard,Analytical Chemistry

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Enzyme-Based Test Strips for Visual or Photographic Detection and Quantitation of Gaseous Sulfur Mustard
Analytical Chemistry ( IF 6.7 ) Pub Date : 2016-05-09 00:00:00 , DOI: 10.1021/acs.analchem.6b01272
Sarka Bidmanova _{1,

2,

3} , Mark-Steven Steiner ₄ , Martin Stepan ₅ , Kamila Vymazalova ₅ , Michael A. Gruber ₆ , Axel Duerkop ₄ , Jiri Damborsky _{1,

2,

3} , Zbynek Prokop _{1,

2,

3} , Otto S. Wolfbeis ₄

Affiliation

Sulfur mustard is a chemical agent of high military and terroristic significance. No effective antidote exists, and sulfur mustard can be fairly easily produced in large quantity. Rapid field testing of sulfur mustard is highly desirable. Existing analytical devices for its detection are available but can suffer from low selectivity, laborious sample preparation, and/or the need for complex instrumentation. We describe a new kind of test strip for rapid detection of gaseous sulfur mustard that is based on its degradation by the enzyme haloalkane dehalogenase that is accompanied by a change of local pH. This change can be detected using pH indicators contained in the strips whose color changes from blue-green to yellow within 10 min. In addition to visual read-out, we also demonstrate quantitative reflectometric readout by using a conventional digital camera based on red-green-blue data acquisition. Organic haloalkanes, such as 1,2-dichloroethane, have a negligible interfering effect. The visual limit of detection is 20 μg/L, and the one for red-green-blue read-out is as low as 3 μg/L. The assays have good reproducibility ±6% and ±2% for interday assays and intraday assays, respectively. The strips can be stored for at least 6 months without loss of function. They are disposable and can be produced fairly rapidly and at low costs. Hence, they represent a promising tool for in-field detection of sulfur mustard.

中文翻译：

基于酶的试纸，用于气态芥菜的目测或照相检测和定量

芥菜酱是具有重要军事和恐怖主义意义的化学制剂。没有有效的解毒剂，硫芥末可以相当容易地大量生产。迫切需要对硫芥末进行快速现场测试。现有的用于其检测的分析装置是可用的，但是会遭受选择性低，样品制备费力和/或需要复杂仪器的困扰。我们描述了一种快速检测气态芥菜气的新型试纸，它基于卤代烷脱卤酶的降解并伴有局部pH值的变化。可以使用试纸条中包含的pH指示剂检测到这种变化，试纸的颜色在10分钟内从蓝绿色变为黄色。除了视觉读取之外，我们还通过使用基于红绿蓝数据采集的常规数码相机演示了定量反射法读数。有机卤代烷烃（例如1,2-二氯乙烷）的干扰作用可忽略不计。检测的视觉极限为20μg/ L，红绿蓝读数的极限值低至3μg/ L。该测定法对于日间测定法和日内测定法分别具有±6％和±2％的良好再现性。试纸条可以保存至少6个月而不会失去功能。它们是一次性的，可以相当快速且低成本地生产。因此，它们代表了现场检测硫芥末的有前途的工具。红绿蓝读数低至3μg/ L。该测定法对于日间测定法和日内测定法分别具有±6％和±2％的良好再现性。试纸条可以保存至少6个月而不会失去功能。它们是一次性的，可以相当快速且低成本地生产。因此，它们代表了现场检测硫芥末的有前途的工具。红绿蓝读数低至3μg/ L。该测定法对于日间测定法和日内测定法分别具有±6％和±2％的良好再现性。试纸条可以保存至少6个月而不会失去功能。它们是一次性的，可以相当快速且低成本地生产。因此，它们代表了现场检测硫芥末的有前途的工具。

更新日期：2016-05-09

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