Fusobacteria are associated with colorectal cancer (CRC) and are amplified during colorectal carcinogenesis. Compared to the adenoma-carcinoma sequence of carcinogenesis, serrated neoplasm has distinct clinical features and a different molecular background. We aimed to compare the gut microbiome between tubular adenoma (TA) and sessile serrated adenoma/polyp (SSA/P). Patients with TA, SSA/P, or CRC were recruited. Three pieces of colorectal mucosal tissue were obtained from each patient by endoscopic biopsy. 16S rRNA gene pyrosequencing and phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) were performed. Among 26 enrolled patients, 8, 10, and 8 had TA, SSA/P, and CRC, respectively. The relative abundance of Fusobacteria did not differ significantly between the TA and SSA/P groups (4.3% and 1.9%, P = 0.739) but was higher in the CRC group (33.8%) than in the TA or SSA/P group, respectively (TA vs. CRC, P = 0.002, false discovery rate [FDR] = 0.023; SSA/P vs. CRC, P < 0.001, FDR = 0.001). PICRUSt revealed that most functions in the TA metagenome were similar to those in the SSA/P metagenome. The gut microbiome, including relative abundance of Fusobacteria, did not differ between TA and SSA/P, suggesting that Fusobacteria may contribute to both the serrated pathway and the adenoma-carcinoma sequence.

中文翻译：

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梭菌在大肠癌发生的锯齿状路径中的作用。

梭菌与大肠癌（CRC）相关，并在大肠癌变过程中被放大。与癌变的腺瘤-癌序列相比，锯齿状肿瘤具有独特的临床特征和不同的分子背景。我们旨在比较肾小管腺瘤（TA）和无柄锯齿状腺瘤/息肉（SSA / P）之间的肠道微生物组。招募患有TA，SSA / P或CRC的患者。通过内窥镜活检从每位患者获得三块结肠直肠粘膜组织。进行了16S rRNA基因焦磷酸测序和通过未观察到的状态重建（PICRUSt）对群落进行的系统发育研究。在26名入选患者中，分别有8名，10名和8名患有TA，SSA / P和CRC。在TA和SSA / P组之间，梭菌的相对丰度没有显着差异（4.3％和1.9％，P = 0。739），但在CRC组中分别高于TA或SSA / P组（33.8％）（TA vs. CRC，P = 0.002，错误发现率[FDR] = 0.023； SSA / P vs. CRC， P <0.001，FDR = 0.001）。PICRUSt揭示了TA元基因组中的大多数功能与SSA / P元基因组中的功能相似。TA和SSA / P之间的肠道微生物组，包括相对丰富的Fusobacteria，没有差异，这表明Fusobacteria可能对锯齿状途径和腺瘤-癌序列都有贡献。