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Photosensitizer-Conjugated Ultrasmall Carbon Nanodots as Multifunctional Fluorescent Probes for Bioimaging
The Journal of Physical Chemistry C ( IF 3.3 ) Pub Date : 2016-04-11 00:00:00 , DOI: 10.1021/acs.jpcc.5b11721 Alexandre Loukanov 1, 2 , Ryota Sekiya 1 , Midori Yoshikawa 1 , Naritaka Kobayashi 1 , Yuji Moriyasu 1 , Seiichiro Nakabayashi 1
The Journal of Physical Chemistry C ( IF 3.3 ) Pub Date : 2016-04-11 00:00:00 , DOI: 10.1021/acs.jpcc.5b11721 Alexandre Loukanov 1, 2 , Ryota Sekiya 1 , Midori Yoshikawa 1 , Naritaka Kobayashi 1 , Yuji Moriyasu 1 , Seiichiro Nakabayashi 1
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Highly luminescent ultrasmall carbon nanodots (CDs) have been prepared by one-step microwave-assisted pyrolysis and functionalized with fluorescein photosensitizer by a diazo-bond. The absorption edge of such prepared fluorescein–N═N–CDs was red-shifted in comparison with the bare one. Nevertheless, the emission signal induced by the nanoparticle quantum-sized graphite structure was quenched due to photoisomerization of the diazo group at the photoexcited state. In order to restrict the photoisomerization, i.e., rotation around the nitrogen–nitrogen bond, the diazo group was fixed by a metal cation to form a complex compound or chelate. The obtained metal complex of fluorescein–N═N–CDs shows an absorbance maximum the same as bare CDs but a recovered emission signal from the nanoparticle moiety, which was bathochromically shifted. They exhibit lower quantum yield in comparison with the bare CDs but better photostability toward emission quenching in nutrition cell culture. The formed photosensitizer-conjugated nanoprobes were proposed as multifunctional fluorophores for intracellular in vivo imaging due to their attractive photophysical attributes and tunable and excitation-dependent emission. The bioapplication of photosensitizer-conjugated CDs was demonstrated as fluorescent tracers for endocytosis pathways in cultured Tobacco cells. Their successful staining and lower toxicity to the plant cells were compared with conventional quantum dots (CdSe/ZnS core–shell type, which caused an acute toxicological in vivo effect).
中文翻译：
光敏剂结合的超小碳纳米点作为生物成像的多功能荧光探针。
通过一步微波辅助热解制备了高发光的超小碳纳米点（CD），并通过重氮键将其与荧光素光敏剂官能化。与裸露的荧光素相比，这种制备的荧光素-N═N-CDs的吸收边缘发生了红移。然而，由于在光激发态下重氮基团的光异构化，由纳米粒子量子尺寸的石墨结构诱导的发射信号被猝灭。为了限制光致异构化，即围绕氮-氮键的旋转，重氮基团被金属阳离子固定，形成络合物或螯合物。所获得的荧光素-N═N-CDs金属配合物显示出的最大吸收值与裸露的CDs相同，但是从纳米颗粒部分回收的发射信号发生了红移。与裸露的CD相比，它们具有较低的量子产率，但在营养细胞培养中对发射猝灭具有更好的光稳定性。形成的光敏剂共轭的纳米探针被提议为细胞内的多功能荧光团由于它们具有吸引人的光物理属性以及可调的和依赖激发的发射，因此可以进行体内成像。结合有光敏剂的CD的生物应用已被证明是培养的烟草细胞中内吞途径的荧光示踪剂。将它们的成功染色和对植物细胞的较低毒性与常规量子点（CdSe / ZnS核-壳型，引起了体内急性毒理学作用）进行了比较。
更新日期：2016-04-11
中文翻译：
光敏剂结合的超小碳纳米点作为生物成像的多功能荧光探针。
通过一步微波辅助热解制备了高发光的超小碳纳米点（CD），并通过重氮键将其与荧光素光敏剂官能化。与裸露的荧光素相比，这种制备的荧光素-N═N-CDs的吸收边缘发生了红移。然而，由于在光激发态下重氮基团的光异构化，由纳米粒子量子尺寸的石墨结构诱导的发射信号被猝灭。为了限制光致异构化，即围绕氮-氮键的旋转，重氮基团被金属阳离子固定，形成络合物或螯合物。所获得的荧光素-N═N-CDs金属配合物显示出的最大吸收值与裸露的CDs相同，但是从纳米颗粒部分回收的发射信号发生了红移。与裸露的CD相比，它们具有较低的量子产率，但在营养细胞培养中对发射猝灭具有更好的光稳定性。形成的光敏剂共轭的纳米探针被提议为细胞内的多功能荧光团由于它们具有吸引人的光物理属性以及可调的和依赖激发的发射，因此可以进行体内成像。结合有光敏剂的CD的生物应用已被证明是培养的烟草细胞中内吞途径的荧光示踪剂。将它们的成功染色和对植物细胞的较低毒性与常规量子点（CdSe / ZnS核-壳型，引起了体内急性毒理学作用）进行了比较。
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