Lignin Alkylation Enhances Enzymatic Hydrolysis of Lignocellulosic Biomass,Energy & Fuels

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Lignin Alkylation Enhances Enzymatic Hydrolysis of Lignocellulosic Biomass
Energy & Fuels ( IF 5.2 ) Pub Date : 2017-10-31 00:00:00 , DOI: 10.1021/acs.energyfuels.7b02405
Chenhuan Lai _{1,

2} , Maobing Tu _{2,

3} , Changlei Xia ₃ , Zhiqiang Shi ₂ , Shaolong Sun _{3,

4} , Qiang Yong ₁ , Shiyuan Yu ₁

Affiliation

Understanding the interactions between lignin and cellulase will help better design biomass pretreatment and enzymatic hydrolysis processes. Ethanol organosolv lignins (EOLs) were isolated from organosolv pretreatment of sweetgum (SG) and loblolly pine (LP) under different temperatures. The structural changes of EOLs were determined by ¹³C NMR and heteronuclear multiple bond correlation NMR to elucidate their positive and/or negative effects on enzymatic hydrolysis. Alkylation of lignin with ethanol resulting in the etherification of C_α was observed in both EOL-SG and EOL-LP. The effect of EOL-SG on enzymatic hydrolysis was correlated with the content of ethoxyl groups. The lignin alkylation in EOLs appeared to be reversible at high temperature, resulting in the inverse change of their effects on enzymatic hydrolysis. A strong correlation was observed between the binding affinity of cellulases onto EOLs and their corresponding hydrolysis yields. Lignin alkylation likely reduced the affinity of enzyme onto lignin, which can enhance the enzymatic hydrolysis.

中文翻译：

木质素烷基化增强木质纤维素生物质的酶水解

了解木质素和纤维素酶之间的相互作用将有助于更好地设计生物质预处理和酶促水解过程。乙醇有机溶剂木质素（EOLs）是在不同温度下从甘蔗（SG）和火炬松（LP）的有机溶剂预处理中分离得到的。通过¹³ C NMR和异核多键相关NMR确定EOL的结构变化，以阐明其对酶促水解的正作用和/或负作用。木质素的烷基化，用乙醇导致C的醚化_α在EOL-SG和EOL-LP中均观察到。EOL-SG对酶水解的影响与乙氧基的含量有关。EOL中的木质素烷基化在高温下似乎是可逆的，从而导致其对酶促水解作用的逆向变化。观察到纤维素酶对EOL的结合亲和力与它们相应的水解产量之间有很强的相关性。木质素烷基化可能会降低酶对木质素的亲和力，从而增强酶促水解作用。

更新日期：2017-10-31

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