Improved Method of Fluorescence Quantum Yield Determination,Analytical Chemistry

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Improved Method of Fluorescence Quantum Yield Determination
Analytical Chemistry ( IF 6.7 ) Pub Date : 2017-08-16 00:00:00 , DOI: 10.1021/acs.analchem.7b02013
Krzysztof Nawara ₁ , Jacek Waluk _{1,

2}

Affiliation

In the most widely used procedure for luminescence quantum yield determination, absorption and emission spectra are measured on two different instruments. This leads to errors caused by wavelength misalignment and different monochromator characteristics of the spectrophotometer and the spectrofluorometer. These errors can be avoided using a method for fluorescence quantum yield determination that relies on simultaneous absorption and fluorescence emission (SAFE) measurement using a single commercial spectrofluorometer. The method’s performance is compared with the standard routinely used procedure for the relative quantum yield determination. The advantages of SAFE measurement are discussed. The proposed novel approach eliminates a number of potential errors in quantum yield determination protocol and provides higher accuracy.

中文翻译：

荧光量子产率测定的改进方法

在用于发光量子产率测定的最广泛使用的程序中，在两种不同的仪器上测量吸收光谱和发射光谱。这导致由波长未对准以及分光光度计和分光荧光计的不同单色仪特性引起的误差。使用用于测定荧光量子产率的方法可以避免这些错误，该方法依赖于使用单个商用分光荧光计同时进行的吸收和荧光发射（SAFE）测量。该方法的性能与用于相对量子产率测定的标准常规程序进行了比较。讨论了SAFE测量的优点。所提出的新颖方法消除了量子产率确定协议中的许多潜在错误，并提供了更高的准确性。

更新日期：2017-08-16

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