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Exclusive Extracellular Bioreduction of Methyl Orange by Azo Reductase-Free Geobacter sulfurreducens
Environmental Science & Technology ( IF 10.8 ) Pub Date : 2017-07-14 00:00:00 , DOI: 10.1021/acs.est.7b02122 Yi-Nan Liu 1 , Feng Zhang 1 , Jie Li 1 , Dao-Bo Li 1 , Dong-Feng Liu 1 , Wen-Wei Li 1 , Han-Qing Yu 1
Environmental Science & Technology ( IF 10.8 ) Pub Date : 2017-07-14 00:00:00 , DOI: 10.1021/acs.est.7b02122 Yi-Nan Liu 1 , Feng Zhang 1 , Jie Li 1 , Dao-Bo Li 1 , Dong-Feng Liu 1 , Wen-Wei Li 1 , Han-Qing Yu 1
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Azo dyes are a class of recalcitrant organic pollutants causing severe environmental pollution. For their biodecolorization, the azo reductase system was considered as the major molecular basis in bacteria. However, the intracellular localization of azo reductase limits their function for efficient azo dye decolorization. This limitation may be circumvented by electrochemically active bacteria (EAB) which is capable of extracellular respiration. To verify the essential role of extracellular respiration in azo dye decolorization, Geobacter sulfurreducens PCA, a model EAB, was used for the bioreduction of methyl orange (MO), a typical azo dye. G. sulfurreducens PCA efficiently reduced MO into amines. Kinetic results showed that G. sulfurreducens PCA had the highest decolorization efficiency among the currently known MO reducing bacteria. Electrons from acetate oxidization by this strain were transferred by the respiratory chain to MO. The mass and electron balances, fluorescent probing and proteinase K treatment experimental results indicate that the biodecolorization of MO by G. sulfurreducens PCA is an exclusive extracellular process. OmcB, OmcC and OmcE were identified as the key outer-membrane proteins for the extracellular MO reduction. This work deepens our understanding of EAB physiology and is useful for the decontamination of environments polluted with azo dyes. The contribution of extracellular respiration to pollutants reduction will broaden the environmental applications of EAB.
中文翻译：
不含偶氮还原酶的Geobacter sulfreducens对甲基橙的独家胞外生物还原
偶氮染料是一类难降解的有机污染物，会造成严重的环境污染。对于它们的生物脱色，偶氮还原酶系统被认为是细菌的主要分子基础。但是，偶氮还原酶在细胞内的定位限制了它们对有效偶氮染料脱色的功能。能够通过细胞外呼吸的电化学活性细菌（EAB）可以规避此限制。为了验证胞外呼吸在偶氮染料脱色中的重要作用，使用了EAB型减细菌Geobacter sulfreducens PCA来对典型的偶氮染料甲基橙（MO）进行生物还原。G.硫还原剂PCA有效地将MO还原为胺。动力学结果表明，G.thioreducens在目前已知的减少MO的细菌中，PCA具有最高的脱色效率。该菌株通过乙酸盐氧化产生的电子通过呼吸链转移到MO。质量和电子平衡，荧光探测和蛋白酶K处理的实验结果表明，G. sulphurreducens PCA对MO的生物脱色是唯一的细胞外过程。OmcB，OmcC和OmcE被确定为降低细胞外MO的关键外膜蛋白。这项工作加深了我们对EAB生理学的理解，可用于对被偶氮染料污染的环境进行净化。细胞外呼吸对减少污染物的贡献将拓宽EAB的环境应用范围。
更新日期：2017-07-14
中文翻译：
不含偶氮还原酶的Geobacter sulfreducens对甲基橙的独家胞外生物还原
偶氮染料是一类难降解的有机污染物，会造成严重的环境污染。对于它们的生物脱色，偶氮还原酶系统被认为是细菌的主要分子基础。但是，偶氮还原酶在细胞内的定位限制了它们对有效偶氮染料脱色的功能。能够通过细胞外呼吸的电化学活性细菌（EAB）可以规避此限制。为了验证胞外呼吸在偶氮染料脱色中的重要作用，使用了EAB型减细菌Geobacter sulfreducens PCA来对典型的偶氮染料甲基橙（MO）进行生物还原。G.硫还原剂PCA有效地将MO还原为胺。动力学结果表明，G.thioreducens在目前已知的减少MO的细菌中，PCA具有最高的脱色效率。该菌株通过乙酸盐氧化产生的电子通过呼吸链转移到MO。质量和电子平衡，荧光探测和蛋白酶K处理的实验结果表明，G. sulphurreducens PCA对MO的生物脱色是唯一的细胞外过程。OmcB，OmcC和OmcE被确定为降低细胞外MO的关键外膜蛋白。这项工作加深了我们对EAB生理学的理解，可用于对被偶氮染料污染的环境进行净化。细胞外呼吸对减少污染物的贡献将拓宽EAB的环境应用范围。
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