当前位置:
X-MOL 学术
›
Nat. Commun.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Generation and comparison of CRISPR-Cas9 and Cre-mediated genetically engineered mouse models of sarcoma.
Nature Communications ( IF 14.7 ) Pub Date : 2017-07-10 , DOI: 10.1038/ncomms15999
Jianguo Huang 1 , Mark Chen 2, 3 , Melodi Javid Whitley 2, 3 , Hsuan-Cheng Kuo 2 , Eric S Xu 1 , Andrea Walens 2 , Yvonne M Mowery 1 , David Van Mater 4 , William C Eward 5 , Diana M Cardona 6 , Lixia Luo 1 , Yan Ma 1 , Omar M Lopez 2 , Christopher E Nelson 7, 8 , Jacqueline N Robinson-Hamm 7, 8 , Anupama Reddy 8 , Sandeep S Dave 8, 9 , Charles A Gersbach 7, 8 , Rebecca D Dodd 1 , David G Kirsch 1, 2
Nature Communications ( IF 14.7 ) Pub Date : 2017-07-10 , DOI: 10.1038/ncomms15999
Jianguo Huang 1 , Mark Chen 2, 3 , Melodi Javid Whitley 2, 3 , Hsuan-Cheng Kuo 2 , Eric S Xu 1 , Andrea Walens 2 , Yvonne M Mowery 1 , David Van Mater 4 , William C Eward 5 , Diana M Cardona 6 , Lixia Luo 1 , Yan Ma 1 , Omar M Lopez 2 , Christopher E Nelson 7, 8 , Jacqueline N Robinson-Hamm 7, 8 , Anupama Reddy 8 , Sandeep S Dave 8, 9 , Charles A Gersbach 7, 8 , Rebecca D Dodd 1 , David G Kirsch 1, 2
Affiliation
![]() |
Genetically engineered mouse models that employ site-specific recombinase technology are important tools for cancer research but can be costly and time-consuming. The CRISPR-Cas9 system has been adapted to generate autochthonous tumours in mice, but how these tumours compare to tumours generated by conventional recombinase technology remains to be fully explored. Here we use CRISPR-Cas9 to generate multiple subtypes of primary sarcomas efficiently in wild type and genetically engineered mice. These data demonstrate that CRISPR-Cas9 can be used to generate multiple subtypes of soft tissue sarcomas in mice. Primary sarcomas generated with CRISPR-Cas9 and Cre recombinase technology had similar histology, growth kinetics, copy number variation and mutational load as assessed by whole exome sequencing. These results show that sarcomas generated with CRISPR-Cas9 technology are similar to sarcomas generated with conventional modelling techniques and suggest that CRISPR-Cas9 can be used to more rapidly generate genotypically and phenotypically similar cancers.
中文翻译:
CRISPR-Cas9 和 Cre 介导的肉瘤基因工程小鼠模型的生成和比较。
采用位点特异性重组酶技术的基因工程小鼠模型是癌症研究的重要工具,但可能成本高昂且耗时。CRISPR-Cas9系统已被改造用于在小鼠体内产生原生肿瘤,但这些肿瘤与传统重组酶技术产生的肿瘤相比如何仍有待充分探索。在这里,我们使用 CRISPR-Cas9 在野生型和基因工程小鼠中有效产生原发性肉瘤的多种亚型。这些数据表明 CRISPR-Cas9 可用于在小鼠体内产生软组织肉瘤的多种亚型。通过全外显子组测序评估,使用 CRISPR-Cas9 和 Cre 重组酶技术产生的原发性肉瘤具有相似的组织学、生长动力学、拷贝数变异和突变负荷。这些结果表明,用 CRISPR-Cas9 技术生成的肉瘤与用传统建模技术生成的肉瘤相似,表明 CRISPR-Cas9 可用于更快速地生成基因型和表型相似的癌症。
更新日期:2017-07-10
中文翻译:

CRISPR-Cas9 和 Cre 介导的肉瘤基因工程小鼠模型的生成和比较。
采用位点特异性重组酶技术的基因工程小鼠模型是癌症研究的重要工具,但可能成本高昂且耗时。CRISPR-Cas9系统已被改造用于在小鼠体内产生原生肿瘤,但这些肿瘤与传统重组酶技术产生的肿瘤相比如何仍有待充分探索。在这里,我们使用 CRISPR-Cas9 在野生型和基因工程小鼠中有效产生原发性肉瘤的多种亚型。这些数据表明 CRISPR-Cas9 可用于在小鼠体内产生软组织肉瘤的多种亚型。通过全外显子组测序评估,使用 CRISPR-Cas9 和 Cre 重组酶技术产生的原发性肉瘤具有相似的组织学、生长动力学、拷贝数变异和突变负荷。这些结果表明,用 CRISPR-Cas9 技术生成的肉瘤与用传统建模技术生成的肉瘤相似,表明 CRISPR-Cas9 可用于更快速地生成基因型和表型相似的癌症。