<br>2-羟基-4-甲氧基苯甲醛是一种比香草醛及其衍生物更有效的抗真菌香气，可破坏细胞膜，抑制 DON 生物合成，对小麦籽粒具有良好的抗真菌作用,Frontiers in Microbiology

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2-羟基-4-甲氧基苯甲醛是一种比香草醛及其衍生物更有效的抗真菌香气，可破坏细胞膜，抑制 DON 生物合成，对小麦籽粒具有良好的抗真菌作用
Frontiers in Microbiology ( IF 4.0 ) Pub Date : 2024-02-26 , DOI: 10.3389/fmicb.2024.1359947
Qian Li _{1,

2} , Chong Wang _{1,

2} , Hongying Xiao _{1,

2} , Yiming Zhang _{1,

2} , Yanli Xie _{1,

2}

Affiliation

禾谷镰刀菌（禾谷镰刀菌）是威胁农业和食品安全的严重病原体。本研究旨在探讨几种植物源性天然化合物（香草醛及其衍生物）对真菌生长的抗真菌功效。禾谷镰刀菌并研究了最强的2-羟基-4-甲氧基苯甲醛（HMB）的抗真菌机制。 HMB抑制菌丝生长的最低抑菌浓度(MIC)为200 μg/mL。 MIC 处的 HMB 通过将渗透性增加约 6 倍来破坏细胞膜（ p < 0.05)，通过碘化丙啶 (PI) 染色证明。同时，在MIC下，HMB处理后丙二醛（MDA）和甘油含量分别增加了45.91%和576.19%，表明细胞膜发生了脂质氧化和渗透应激。此外，HMB 发挥了强大的抗毒作用，第 7 天时脱氧雪腐镰刀菌烯醇 (DON) 的含量在 MIC 下显着降低了 93.59%。最后，HMB的抗真菌作用禾谷镰刀菌小麦籽粒也得到证实。这些结果不仅揭示了HMB的抗真菌机制，而且表明HMB可以作为一种有前景的抗真菌剂应用于农产品保鲜中。

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2-Hydroxy-4-methoxybenzaldehyde, a more effective antifungal aroma than vanillin and its derivatives against Fusarium graminearum, destroys cell membranes, inhibits DON biosynthesis, and performs a promising antifungal effect on wheat grains

Fusarium graminearum (F. graminearum) is a severe pathogen threatening the safety of agriculture and food. This study aimed to explore the antifungal efficacies of several plant-derived natural compounds (vanillin and its derivatives) against the growth of F. graminearum and investigate the antifungal mechanism of 2-hydroxy-4-methoxybenzaldehyde (HMB), the strongest one. The minimum inhibitory concentration (MIC) of HMB in inhibiting mycelial growth was 200 μg/mL. HMB at MIC damaged cell membranes by increasing the permeability by about 6-fold (p < 0.05) as evidenced by propidium iodide (PI) staining. Meanwhile, the content of malondialdehyde (MDA) and glycerol was increased by 45.91 and 576.19% by HMB treatment at MIC, respectively, indicating that lipid oxidation and osmotic stress occurred in the cell membrane. Furthermore, HMB exerted a strong antitoxigenic role as the content of deoxynivalenol (DON) was remarkably reduced by 93.59% at MIC on 7th day. At last, the antifungal effect of HMB against F. graminearum was also confirmed on wheat grains. These results not only revealed the antifungal mechanism of HMB but also suggested that HMB could be applied as a promising antifungal agent in the preservation of agricultural products.

更新日期：2024-02-26

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