A simple and sensitive colorimetric assay for detecting organophosphorus pesticides (OPs) was developed based on 3,3',5,5'-tetramethylbenzidine (TMB)/hydrogen peroxide (H₂O₂)/dodecyl trimethylammonium bromide (DTAB)-tetramethyl zinc (4-pyridinyl) porphyrin (ZnTPyP). In this system, based on the peroxidase-like activity of DTAB-ZnTPyP, H₂O₂ decomposes to produce hydroxyl radicals, which oxidize TMB, resulting in blue oxidation products. The OPs (trichlorfon, dichlorvos, and thimet) were first combined with DTAB-ZnTPyP through electrostatic interactions. The OPs caused a decrease in the peroxidase-like activity of DTAB-ZnTPyP due to spatial site blocking. At the same time, π-interactions occurred between them, and these interactions also inhibited the oxidation of TMB (652 nm), thus making the detection of OPs possible. The limits of detection for trichlorfon, dichlorvos, and thimet were 0.25, 1.02, and 0.66 μg/L, respectively, and the corresponding linear ranges were 1–35, 5–45, and 1–40 μg/L, respectively. Moreover, the assay was successfully used to determine OPs in cabbage, apple, soil, and traditional Chinese medicine samples (the recovery ratios were 91.8–109.8%), showing a great promising potential for detecting OPs also in other complex samples.

基于3,3',5,5'-四甲基联苯胺 (TMB)/过氧化氢 (H ₂ O ₂ )/十二烷基三甲基溴化铵 (DTAB)-四甲基锌开发了一种简单灵敏的比色法检测有机磷农药 (OPs) (4-吡啶基)卟啉 (ZnTPyP)。在该系统中，基于 DTAB-ZnTPyP 的过氧化物酶样活性，H ₂ O ₂分解产生羟基自由基，将TMB氧化，产生蓝色氧化产物。OP（敌百虫、敌敌畏和噻吩）首先通过静电相互作用与 DTAB-ZnTPyP 结合。由于空间位点阻塞，OPs 导致 DTAB-ZnTPyP 的过氧化物酶样活性降低。同时，它们之间发生了π-相互作用，这些相互作用也抑制了TMB（652 nm）的氧化，从而使OPs的检测成为可能。敌百虫、敌敌畏和噻吩噻吩的检出限分别为 0.25、1.02 和 0.66 μg/L，对应的线性范围分别为 1-35、5-45 和 1-40 μg/L。此外，该方法成功地用于测定白菜、苹果、土壤和中药样品中的 OPs（回收率为 91.8-109.8%），