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成果及论文

独立研究成果：

2024

1. Shaixiao Tian,  Xinying Ge, Qipeng Yan, Min Li, Qun Huang, Xinhua Zhang, Ming Ma, Bo Chen, and Jian-bo Wang*. "Directed evolution of stereoselective enzymes meets click reactions: Asymmetric synthesis of chiral triazoles using a Cu(I)-compatible halohydrin dehalogenase". Green Synthesis and Catalysis. 2024. 01

https://doi.org/10.1016/j.gresc.2024.01.001




2023

1. Wei Tong#, Qipeng Yan#, Shaixiao Tian#, Ruihai Tang, Bo Chen, and Jian-bo Wang*. "Single-site Modification of the P450-BM3 Substrate-entrance Facilitates the Synthesis of Optically Pure Pharmaceutically Useful Metheyl Trans-3-phenylglycidates" Molecular Catalysis. 2023, 547, 113354. (#并列一作）

https://doi.org/10.1016/j.mcat.2023.113354


2. 王翠珍, 陈窕, 王健博。"酶催化杂Diels-Alder反应". 合成生物学, 2024, 5 : 107-125.

Cuizhen Wang， Tiao Chen，and Jian-bo Wang*. "Enzyme-catalyzed Hetero-Diels-Alder Reactions". Synthetic Biology Journal，2024, 5, 107-125.

https://synbioj.cip.com.cn/CN/10.12211/2096-8280.2023-015

2022

1. Qipeng Yan, Xinhua Zhang, Yingzhuang Chen, Bin Guo, Pei Zhou, Bo Chen, Qun Huang*, and Jian-bo Wang*. " From Semirational to Rational Design: Developing a Substrate-Coupled System of Glucose Dehydrogenase for Asymmetric

Synthesis". ACS Catalysis. 2022, 12, 6746−6755.

https://doi.org/10.1021/acscatal.2c00705


2. Qun Huang#, Xuan Zhang#, Qianqian Chen, Shaixiao Tian, Wei Tong, Wei Zhang, Yingzhuang Chen, Ming Ma, Bo Chen, Binju Wang, and Jian-bo Wang*. "Discovery of a P450-Catalyzed Oxidative Defluorination Mechanism toward Chiral Organofluorines: Uncovering a Hidden Pathway". ACS Catalysis. 2022, 12, 265−272. (#并列一作）

https://doi.org/10.1021/acscatal.1c05510


2021

1. Zexing Wen#, Zhi-Min Zhang#, Liang Zhong#, Jiaqian Fan, Min Li, Yuanhong Ma, Yang Zhou, Wei Zhang, Bin Guo, Bo Chen, and Jian-Bo Wang*. "Directed Evolution of a Plant Glycosyltransferase for Chemo- and Regioselective Glycosylation of Pharmaceutically Significant Flavonoids". ACS Catalysis. 2021, 11, 14781−14790. (#并列一作）

https://doi.org/10.1021/acscatal.1c04191

2. Yue Yue#, Jiaqian Fan#, Guoqing Xin, Qun Huang, Jian-bo Wang*, Yanwei Li*, Qingzhu Zhang, and  Wenxing Wang. "Comprehensive Understanding of Fluoroacetate Dehalogenase-Catalyzed Degradation of Fluorocarboxylic Acids: A QM/MM Approach". Environment Science & Techanology. 2021, 55 (14), 9817–9825. (#并列一作）

https://doi.org/10.1021/acs.est.0c08811

Abstract Image

3. Mi Liu, Dan Wei, Zexing Wen and Jian-bo Wang*. "Progress in Stereoselectiv Construction of C–C Bonds Enabled by Aldolases and Hydroxynitrile Lyases". Frontiers in Bioengineering and Biotechnology. 2021, 9, 297.

https://doi.org/10.3389/fbioe.2021.653682

2020

1. Shu Wang, Zhanbing Cheng, Yanbing Xu, Lu Yang, Jian-bo Wang, Zhenhua Tian* and Xudong Qu*. "Structure-guided protein design of fluoroacetate dehalogenase for kinetic resolution of rac-2-bromobutyric acid". Green Synthesis and Catalysis. 2020, 1, 60-65.

https://doi.org/10.1016/j.gresc.2020.05.004

                                                 
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2. Hongxia Zhang#, Shaixiao Tian#, Yue Yue, Min Li, Wei Tong, Guangyu Xu, Bo Chen, Ming Ma, Yanwei Li* and Jian-bo Wang*. "Semirational Design of Fluoroacetate Dehalogenase RPA1163 for Kinetic Resolution of α-Fluorocarboxylic Acids on a Gram Scale". ACS Catalysis. 2020, 10, 3143-3151. (#并列一作）

https://doi.org/10.1021/acscatal.9b04804

3. Jian-bo Wang*#, Qun Huang#, Wei Peng, Peng Wu, Da Yu, Bo Chen, Binju Wang*, and Manfred T. Reetz*. "P450-BM3-Catalyzed Sulfoxidation versus Hydroxylation: A Common or Two Different Catalytically Active Species?". Journal of the American Chemical Society. 2020, 142 (4), 2068–2073. (#并列一作)

https://doi.org/10.1021/jacs.9b13061

   

2019

1. Wei Tong, Qun Huang, Min Li, and Jian-bo Wang*.  "Enzyme-catalyzed C–F bond formation and cleavage". Bioresources and Bioprocessing, 2019, 6 (1), 1-8.

https://doi.org/10.1186/s40643-019-0280-6

2. Yanwei Li*, Yue Yue, Hongxia Zhang, Zhongyue Yang, Hui Wang , Shaixiao Tian, Jian-bo Wang* , Qingzhu Zhang*, Wenxing Wang. "Harnessing fluoroacetate dehalogenase for defluorination of fluorocarboxylic acids: in silico and in vitro approach". Enviroment International. 2019, 131, 104999.

https://doi.org/10.1016/j.envint.2019.104999

3. Da Yu, Jian-bo Wang* and Manfred. T. Reetz*. "Exploiting Designed Oxidase-Peroxygenase Mutual Benefit System for Asymmetric Cascade Reactions". Journal of the American Chemical Society. 2019, 141 (14), 5655-5658. 

https://doi.org/10.1021/jacs.9b01939

Abstract Image


博士以及博后期间成果： 

1. Guangyue Li, Jian-bo Wang  and Manfred T. Reetz*. "Biocatalysts for the Pharmaceutical Industry created by Structure-guided directed Evolution of Stereoselective Enzymes". Bioorganic & Medicinal Chemistry. 2018, 26 (7), 1241-1251. 
2. Jian-bo Wang, Guangyue Li, and Manfred T.Reetz*. "Enzymatic Site-selectivity Enabled by Structure-guided Directed Evolution". Chemical Communications. 2017, 53,3916-3928.

3. Jian-bo Wang, Adriana Ilie, Shuangguang Yuan* and Manfred T. Reetz*. "Investigating Substrate Scope and Enantioselectivity of a Defluorinase by a Stereochemical Probe". Journal of the American Chemical Society. 2017, 139,11241-11247.
4. Jian-bo Wang, AdrianaIlie, and Manfred T. Reetz*. "Chemo- and Stereoselective P450-BM3 Catalyzed Sulfoxidation of 1-Thiochroman-4-ones Enabled by Directed Evolution". Advanced Synthesis & Catalysis. 2017, 359, 2056-2060.

5. Xin Jia#, Jian-bo Wang#, Shannon Rivera, Duc Duong, and Emily E. Weinert*. "An O2-sensing Stressosome from a Gram-negative Bacterium". Nature Communications. 2016, 7,12381. (#并列一作)
6. Jian-bo Wang, Richard Lonsdale, and Manfred T. Reetz*. "Exploring Substrate Scope and Stereoselectivity of P450 Peroxygenase OleTJE in Olefin-forming Oxidative Decarboxylation". Chemical Communications. 2016, 52, 8131-8133.
7. ZhouTong Sun,Richard Lonsdale, Lian Wu, GuangYue Li, AiTao Li, Jian-Bo Wang, JiaHai Zhou, and Manfred T. Reetz*. "Structure-Guided Triple-Code Saturation Mutagenesis: Efficient Tuning of the Stereoselectivity of an Epoxide Hydrolase". ACS Catalysis. 2016, 6, 1590-1597.
8. Jian-bo Wang, and Manfred T. Reetz*. "Biocatalysis: Chiral Cascades". Nature Chemistry. 2015, 7, 948-949.
9. Jian-bo Wang, Feng Zhang, Jin-Yue Pu, Juan Zhao, Qun-Fei Zhao, and Gong-Li Tang*. "Characterization of AvaR1, an Autoregulator Receptorthat Negatively Controls Avermectins Production in a High Avermectin-Producing Strain". Biotechnology Letters. 2014, 36, 813-819.
10. Wei Huang, Jian-boWang, and Gong-Li Tang*. "Synthetic Biology Toward Medicinal Natural Products". Chinese Bulletin of Life Sciences. 2011, 9, 012.
11. Jian-bo Wang, Hai-Xue Pan, and Gong-Li Tang*. "Production of Doramectin by Rational Engineering of the Avermectin Biosynthetic Pathway". Bioorganic & Medicinal Chemistry Letters. 2011, 21, 3320-3323.

专利

  1. 王健博，文泽星。一种生物催化合成多种黄酮苷的方法。中国专利，专利号：ZL202110046315.X

  2.  唐功利，王健博。利用调控蛋白基因提高阿维菌素的产量。中国专利，专利号： ZL200910196742.5

  3.  唐功利，王健博。利用初级糖代谢基因提高阿维菌素的产量。 中国专利，专利号： ZL200910199530.2

  4.  唐功利，王健博。一种改造除虫链霉菌产生多拉菌素的方法。中国专利，专利号 ：ZL201010568764.2


可制备化合物结构列表

手性纯α-氟代羧酸

手性纯α-羟基羧酸

手性纯环氧肉桂酸甲酯