53. Hou, W.; Xiong, H.; Bai, R.; Xiao, Z.; Su, L.; Ruan, B. H.;* Xu, H.* Synthesis of Indazolo[2,1-a]Cinnolines via Rhodium (III)-Catalyzed C–H activation/annulation under mild conditions. Tetrahedron 2019, 75, 4005-4009. DOI: 10.1016/j.tet.2019.06.021.
52. Hu, Q., Yu, Y., Gu, D., Xie, L., Chen, X., Xu, N., Ruan, J.J., Dowson, C., and Ruan, B.H.* Detection of “hidden” antimicrobial drug resistance. ACS Infect. Dis. 5, 1252-1263 (2019). DOI: 10.1021/acsinfecdis.9b00132.
51. Hou, W.; Zhou. Y.; Rui. J.; Bai, R.; Bhasin, A. K. K.; Ruan, B. H.* Design and synthesis of novel tellurodibenzoic acid compounds as kidneytype glutaminase (KGA) inhibitors. Bioorg. Med. Chem. Lett. 2019, 29, 1673-1676. DOI: 10.1016/j.bmcl.2019.04.032.
50. Ruan JJ., Yu Y., Hou W., Chen Z., Fang J., Zhang J., Ni M., Li D., Lu S., Rui J., Wu R., Zhang W., Ruan BH.* Kidney-Type Glutaminase Inhibitor Hexylselen Selectively Kills Cancer Cells via a Three-Pronged Mechanism. ACS Pharmacol Transl Sci., 2 (1), 18-30 (2019). DOI:10.1021/acsptsci.8b00047
49. Hou W., Lu S., Zhao H., YuY., Xu H., Yu B., Su L., Lin C., Ruan BH.* Propylselen inhibits cancer cell growth by targeting glutamate dehydrogenase at the NADP+ binding site. Biochem Bioph Res Commun., 509, 262-267 (2019). DOI:10.1016/j.bbrc.2018.12.11
48. Chen Z., Li D., Xu N., Fang J., Yu Y., Hou W., Ruan H. Zhu P., Ma R., Lu S., Cao D., Wu R., Ni M., Zhang W., Su W., Ruan BH.* Novel 1,3,4-Selenadiazole-Containing Kidney-Type Glutaminase Inhibitors Showed Improved Cellular Uptake and Antitumor Activity. J. Med. Chem., 62(3), 589-603 (2019). DOI:10.1021/acs.jmedchem.8b01198
47. Su L., Wu R., Chen X., Hou W., Ruan BH.* FITC-labeled D-glucose analog is suitable as a probe for detecting insulin-dependent glucose uptake. Bioorg. Med. Chem. Lett. 28, 3560-3563 (2018). DOI:10.1016/j.bmcl.2018.09.027
46. Jin Y., Li D., Lu S, Zhao H, Chen Z, Hou W, Ruan BH.* Ebselen reversibly inhibits human glutamate dehydrogenase at the catalytic site. Assay Drug Dev Technol., 16(2), 115-122 (2018). DOI:10.1089/adt.2017.822
45. Yu Y, Jin Y, Zhou J, Ruan H, Zhao H, Lu S, Zhang Y, Li D, Ji X, Ruan BH.* Ebselen: mechanisms of glutamate dehydrogenase and glutaminase enzyme inhibition. ACS Chem Bio, 12(12), 3003-3011 (2017). DOI:10.1021/acschembio.7b00728
44. Hou W, Luo Z, Zhang G, Cao D, Li D, Ruan H, Ruan B.H., Su L, Xu H. Click chemistry-based synthesis and anticancer activity evaluation of novel C-14 1,2,3-triazole dehydroabietic acid hybrids. European Journal of Medicinal Chemistry, 138, 1042-1052 (2017). DOI:10.1016/j.ejmech.2017.07.049
43. Wang, W., Shao, A., Zhang, N., Fang, J., Ruan J.J. and Ruan, B.H.* Cationic polymethacrylate-modified liposomes significantly enhanced doxorubicin delivery and antitumor activity. Scientific Reports, 1(1), 43036-43045 (2017). DOI: 10.1038/srep43036.
42. Zhu, M., Fang, J., Zhang, J., Zhang, Z., Xie, J., Yu, Y., Ruan, J.J., Chen, Z., Hou, W., Yang, G., Su, W., Ruan, B.H.* Biomocular Interaction assays identified novel dual inhibitors of glutaminase and glutamate dehydrogenase that disrupt mitochondrial function and prevent growth of cancer cells. Anal Chem. 89, 1689-1696 (2017). DOI:10.1021/acs.analchem.6b03849.
41. Zhang, W.; Zhu, M.; Wang, F.; Cao, D.; Su, W.; Ruan, B.H.* Mono-sulfonated tetrazolium salt based NAD(P)H detection reagents suitable for dehydrogenase and real-time cell viability assays. Anal. Biochem. 509, 33-40 (2016). DOI:10.1016/j.ab.2016.06.026
40. Zhang, W., Ding, Q., Ruan, J. J., Fang, J., Ruan, B.H.* Biomolecular Interactions and Application of Carbon Nanotubes in Nanomedicine. Austin Biomol Open Access, 1, 1005-1010 (2016)
39. Zhang, W., Zhu, M., Wang, F., Cao, D., Ruan, J. J., Su, W., and Ruan, B.H.* Mono-sulfonatedtetrazolium salt based NAD(P)H detection reagents suitable for dehydrogenase and real-time cell viability assays. Anal. Biochem.,509, 33-40 (2016). DOI:10.1016/j.ab.2016.06.026
38. Chen, X.*, Fang, J., Cheng, Y., Zheng J., Zhang, J., Chen, T., and Ruan, B.H.* Biomolecular Interaction Analysis for Carbon Nanotubes and for Biocompatibility Prediction, Anal. Biochem., 505, 1-7 (2016). DOI:10.1016/j.ab.2016.04.005
37. Dai, J., Ruan, B.H., Zhu, Y., Liang, X., Su, F., and Su, W., Preparation of nanosized Fluticasone Propionate nasal spray with improved stability and uniformity, Chemical Industry and Chemical Engineering Quarterly, 21, 457-464 (2015). DOI:10.2298/CICEQ140609001D.
36. Ruan, B.H., Li, X., Winkler, A, Cunningham, K, Kuai, J., Greco, R., Nocka, K., Fitz, L., Wright, J., Pittman, D., Paulsen, J., Lin, L., and Winkler, D., Complement C3a, CpGoligos and DNA/C3a complex stimulated IFN-a production in a receptor for advanced glycation end product-dependent manner. J. Immunol., 185, 4213-4222 (2010). DOI:10.4049/jimmunol.1000863
35. Ruan, B., Cole, D., Wu P., Quazi, A., Page, K., Wright, J., Huang, N., Stock, J., Nocka, K., Aulabaugh, A., Krykbaev, R., Fitz, L., Wolfman, N., and Fleming, M., A fluorescent assay suitable for inhibitor screening and vanin tissue quantification. Anal. Biochem., 399, 284-292 (2010). DOI:10.1016/j.ab.2009.12.010
34. Li, X., Kuai, J., Cunningham, K., Ruan, B., Fitz, L., Nocka, K., Winkler, A., Paulsen, J., Pittman, D., Lin, L.L., and Winkler, D., Direct interaction of receptor for advanced glycation end products with CPGa and complement component C3a augments interferon alpha in human PBMCs, Cytokine, 48, 106-106 (2009). DOI:10.1016/j.cyto.2009.07.445.
33. Ruan, B., Palioura, S., Sabina, J., Marvin-Guy, L., Kochhar, S., Larossa, R.A., and Söll, D., Quality control despite mistranslation caused by an ambiguous genetic code. Proc. Natl. Acad. Sci. USA., 105, 16502-16507 (2008). DOI: 10.1073/pnas.0809179105.
32. Ruan, B., Pong, K., Jow, F., Bowlby, M., Crozier, R.A., Liu, D., Liang, S., Chen, Y., Mercado, M.L., Feng, X., Bennett, F., von Schack, D., McDonald, L., Zaleska, M.M., Wood, A., Reinhart, P.H., Magolda, R.L., Skotnicki, J., Pangalos, M.N., Koehn, F.E., Carter, G.T., Abou-Gharbia, M. and Graziani, E.I., Binding of rapamycin analogs to calcium channels and FKBP52 contributes to their neuroprotective activities. Proc. Natl. Acad. Sci. USA. 105, 33-38 (2008). DOI: 10.1073/pnas.0710424105.
31. Graziani, E.I., Ruan, B., Liang, S., Liu, D., Jow, F., and Bowlby, M., ORGN 282-Synthesis and chemical biology of ILS-920: A novel non-immunosuppressive rapamycin analog with efficacy in multiple animal models of ischemic stroke, Acs National Meeting Book of Abstracts. 234 (2007)
30. Ruan, B., Bovee, M.L., Sacher, M., Stathopoulos C., Poralla K., Francklyn C.S., and Söll D., A unique hydrophobic cluster near the active site contributes to differences in borrelidin inhibition among threonyl-tRNAsynthetases. J. Biol. Chem. 280, 571-577 (2005). DOI: 10.1074/jbc.M411039200
29. Ruan, B., and Söll, D., The bacterial YbaK protein is a Cys-tRNAProand Cys-tRNACysdeacylase. J. Biol. Chem., 280, 25887-25891 (2005). doi: 10.1074/jbc.M502174200.
28. Ruan, B., Nakanoa, H., Tanaka, M., Mills, J.A., DeVito J. A., Min, B., Low, K.B., Battista, J.R. and Söll, D., Cysteinyl-tRNACys formation in Methanocaldococcusjannaschii, the mechanism is still unknown. J. Bacteriol., 186, 8-14 (2004). DOI: 10.1128/JB.186.1.8-14.2004.
27. Korencic, D., Ahel, I., Schelert, J., Sacher, M., Ruan, B., Stathopoulos, C., Blum, P., Ibba, M., and Söll, D., A freestanding proofreading domain is required for protein synthesis quality control in archaea. Proceedings of the National Academy of Sciences of the United States of America, 101, 10260-10265 (2004). DOI: 10.1073/pnas.0403926101.
26. Ambrogelly, A., Kamtekar, S., Sauerwald, A., Ruan, B., Tumbula-Hansen, D., & Kennedy, D., Ahel, I., and Söll, D., Cys-trnacys, formation and cysteine biosynthesis in methanogenic archaea: two faces of the same problem? Cellular & Molecular Life Sciences Cmls., 61, 2437-2445 (2004) DOI: 10.1007/s00018-004-4194-9
25. Min, B., Kitabatake, M., Polycarpo, C., Pelaschier, J., Raczniak, G., Ruan, B., Kobayashi, H., Namgoong, S., and Söll, D., Protein synthesis in escherichia coli with mischarged trna. Journal of Bacteriology, 185, 3524-3526 (2003) doi: 10.1128/JB.185.12.3583-3595.2003
24. Polycarpo, C., Ambrogelly, A., Ruan, B., Tumbula-Hansen, D., Ataide, S. F., Ishitani, R., Yokoyama, S., Nureki, O., Ibba, M., Söll, D., Activation of the Pyrrolysine Suppressor tRNA Requires Formation of a Ternary Complex with Class I and Class II Lysyl-tRNA Synthetases. Mol Cell, 12, 287-294 (2003) doi:10.1016/S1097-2765(03)00280-6
23. Ruan, B., Lai, P.S., Yeh, C.W., Wilson, W.K., Pang, J., Xu, R., Matsuda, S.P., and Schroepfer, G.J., Jr., Alternative pathways of sterol synthesis in yeast : use of C27, sterol tracers to study aberrant double-bond migrations and evaluate their relative importance. Steroids, 67, 1109-1119 (2002) doi:10.1016/S0039-128X(02)00069-7
22. Ambrogelly, A., Ahel, I., Polycarpo, C., Bunjun-Srihari, S., Krett, B., & Jacquin-Becker, C., Jacquin-Becker C, Ruan B, Köhrer C, Stathopoulos C, RajBhandary UL, and Söll D., Methanocaldococcus jannaschii prolyl-trna synthetase charges trna(pro) with cysteine. Journal of Biological Chemistry, 277, 34749-34754 (2002) doi:10.1074/jbc.M206929200
21. Xiong, Q., Ruan, B., Whitby, F.G., Tuohy, R.P., Belanger, T.L., Kelley, R.I., Wilson, W.K., and Schroepfer G.J., A colorimetric assay for 7-dehydrocholesterol with potential application to screening for smith-lemli-opitz syndrome. Chemistry & Physics of Lipids, 115, 1-15 (2002). doi:10.1016/S0009-3084(01)00205-5
20. Jacquin-Becker, C., Ahel, I., Ambrogelly, A., Ruan, B., Söll, D., and Stathopoulos, C., Cysteinyl-trna formation and prolyl-trna synthetase. Febs Letters, 514, 34-36 (2002) doi:10.1016/S0014-5793(02)02331-1
19. Ruan, B., Wilson, W.K., Pang, J., Gerst, N., Pinkerton, F.D., Tsai, J., Kelley, R.I., Whitby, F.G., Milewicz, D.M., Garbern, J. and Schroepfer, G.J., Jr., Sterols in blood of normal and Smith-Lemli-Opitz subjects. J. Lipid Res., 42, 799-812 (2001) doi:10.1089/107999001300177529
18. Lindenthal, B., Holleran, A. L., Aldaghlas, T. A., Ruan, B., Schroepfer GJ Jr., Wilson, W. K., and Kelleher JK., Progestins block cholesterol synthesis to produce meiosis-activating sterols. Faseb Journal Official Publication of the Federation of American Societies for Experimental Biology, 15, 775-784. (2001)
17. Downs, S. M., Ruan, B., and Schroepfer GJ Jr., Meiosis-activating sterol and the maturation of isolated mouse oocytes. Biology of Reproduction, 64, 80-89 (2001) doi:10.1095/biolreprod64.1.80
16. Ruan, B.F., Ahel, I., Ambrogelly, A., Becker, H.D., Bunjun, S., Feng, L., Tumbula-Hansen D., Ibba, M., Korencic, D., Kobayashi, H., Jacquin-Becker, C., Mejlhede, N., Min, B., Raczniak, G., Rinehart, J., Stathopoulos, C., Li, T., and Söll, D., Genomics and evolution of aminoacyl-trna synthesis. Acta Biochimica Polonica, 48, 313-321 (2001)
15. Stathopoulos, C., Ahel, I., Ali, K., Ambrogelly, A., Becker, H., Bunjun, S., Feng, L., Herring S, Jacquin-Becker C, Kobayashi H, Korencic D, Krett B, Mejlhede N, Min B, Nakano H, Namgoong S, Polycarpo C, Raczniak G, Rinehart J, Rosas-Sandoval G, Ruan B, Sabina J, Sauerwald A, Toogood, H., Tumbula-Hansen, D., Ibba, M., Söll, D., Aminoacyl-trna synthesis: a postgenomic perspective. Cold Spring Harbor Symposia on Quantitative Biology, 66, 175-83 (2001). doi: 10.1101/sqb.2001.66.175
14. Ruan, B., Tsai, J., Wilson, W. K., and Schroepfer, G.J., Aberrant pathways in the late stages of cholesterol biosynthesis in the rat. Origin and metabolic fate of unsaturated sterols relevant to the smith-lemli-opitz syndrome. Journal of Lipid Research, 41, 1772-1782 (2000) doi:10.1089/10799900050198480
13. Ruan, B., Wilson, W.K., Pang, J., and Schroepfer, G.J., Synthesis of [3alpha-3h] cholesta-5,8-dien-3beta-ol and tritium-labeled forms of other sterols of potential importance in the smith-lemli-optiz syndrome. Steroids, 65, 29-39 (2000) doi: 10.1016/S0039-128X(99)00079-3
12. Ruan, B., Wilson, W.K., and Schroepfer, G.J., An improved synthesis of (20R, 22R)-cholest-5-ene-3β,20,22-triol, an intermediate in steroid hormone formation and an activator of nuclear orphan receptor lxrα. Steroids, 64, 385-395 (1999) doi:10.1016/S0039-128X(98)00116-0
11. Ruan, B, Wilson, W.K., and Schroepfer, G.J., J., Cheminform Abstract: An Alternative Synthesis of 4,4-Dimethyl-5α-cholesta-8,14,24-trien-3β-ol, an Intermediate in Sterol Biosynthesis and a Reported Activator of Meiosis and of Nuclear Orphan Receptor LXRα, Cheminform, 29, (1998) doi:10.1016/S0960-894X(98)00016-X
10. Ruan, B., Wilson, W. K., Schroepfer, G.. J., An alternative synthesis of 4,4-dimethyl-5 alpha-cholesta-8,14,24-trien-3 beta-ol, an intermediate in sterol biosynthesis and a reported activator of meiosis and of nuclear orphan receptor LXR alpha. Bioorg. Med. Chem. Lett., 8, 233-236 (1998) doi:10.1016/S0960-894X(98)00016-X
9. Ruan, B., Watanabe, S., Eppig, J.J., Kwoh, C., Dzidic, N., Pang, J., Wilson, W.K., and Schroepfer, G. J., Sterols affecting meiosis: novel chemical syntheses and the biological activity and spectral properties of the synthetic sterols. Journal of Lipid Research, 39, 2005-2020 (1998) doi:10.1089/jir.1998.18.905
8. Ruan, B., Wilson, W. K., and Schroepfer, G. J., Silver ion chromatography and H-1 NMR for sterol analysis: Application to plasma sterols in developmental disorder. FASEB Journal. 12, A1387-A1387 (1998).
7. Ruan, B., Gerst, N., Emmons, G. T., Shey, J., and Schroepfer, G. J., Sterol synthesis. A timely look at the capabilities of conventional and silver ion high performance liquid chromatography for the separation of c27 sterols related to cholesterol biosynthesis. Journal of Lipid Research, 38, 2615-2626 (1997) doi:10.1016/S0929-7855(97)00031-X
6. Forman, B.M., Ruan, B., Chen, J., Schroepfer, G.J., Jr., and Evans, R.M., The orphan nuclear receptor LXRα is positively and negatively regulated by distinct products of mevalonate metabolism. Proc. Natl. Acad. Sci. USA, 94, 10588-10593 (1997) doi:10.1073/pnas.94.20.10588
5. Gerst, N., Ruan, B., Pang, J., Wilson, W. K., and Schroepfer, G.J., Jr., An updated look at the analysis of unsaturated C27 sterols by gas chromatography and mass spectrometry. Journal of Lipid Research, 38, 1685-1701 (1997) doi:10.1089/jir.1997.17.489
4. Ruan, B., Shey, J., Gerst, N., Wilson, W.K., and Schroepfer, G.J., Jr., Silver ion high pressure liquid chromatography provides unprecedented separation of sterols: Application to the enzymatic formation of cholesta-5,8-dien-3β-ol. Proc. Natl. Acad. Sci. USA, 93, 11603-11608 (1996) doi:10.1073/pnas.93.21.11603
3. Ruan, B., Pang, J., Wilson, W.K., & Schroepfer, G.J., Concerning the thermolability of cholesta-5,8-dien-3β-ol, a sterol that accumulates in blood and tissues in a human genetic developmental disorder. Bioorganic & Medicinal Chemistry Letters, 6, 2421–2424 (1996) doi:10.1016/0960-894X(96)00437-4
2. Wilson, W. K., Sumpter, R. M., Warren, J. J., Rogers, P. S., Ruan, B., and Schroepfer, G.J., Jr., Analysis of unsaturated C27 sterols by nuclear magnetic resonance spectroscopy. Journal of Lipid Research, 37, 1529-1555 (1996) doi:10.1021/bi0475830
1. Wang, Z., and Ruan, B., Trifluoromethylation of steroidal ketones. Journal of Fluorine Chemistry, 69, 1-3 (1994). DOI: 10.1016/0022-1139(94)03096-0.
