一. 蛋白质合成技术：

·  Qingyun Zheng^#; Tian Wang^#; Junxiong Mao^#; Guochao Chu; Lujun Liang; Yangwode Jing; Chong Zuo; Yuanyuan Yu^*; Honggang Hu^*; Man Pan^*; A Bifunctional Molecule-Assisted Synthesis of Mimics of Ubiquitin Chain, E2 Enzyme Intermediate, and Deubiquitinase Intermediate. Nature Protocols, 2022, in press. 

·  Qian Qu^#, Shuai Gao^#, Fangming Wu, Meng-Ge Zhang, Ying Li, Long-Hua Zhang, Donald Bierer, Chang-Lin Tian*, Ji-Shen Zheng*, Lei Liu*. Synthesis of disulfide surrogate peptides incorporating large-span surrogate bridges through a native-chemical-ligation-assisted diaminodiacid strategy. Angewandte Chemie International Edition, 2020, 59, 6037-6045. 

·  Man Pan#; Qingyun Zheng#; Shuai Gao; Qian Qu; Yuanyuan Yu; Ming Wu; Huan Lan; Yulei Li; Sanling Liu; Jiabin Li; Demeng Sun; Lining Lu; Tian Wang; Wenhao Zhang; Jiawei Wang; Yiming Li; Hong-Gang Hu＊; Changlin Tian＊; Lei Liu＊; Chemical Synthesis of Structurally Defined Phosphorylated Ubiquitins Suggests Impaired Parkin Activation by Phosphorylated Ubiquitins with a Non-Phosphorylated Distal Unit, CCS Chemistry, 2019, 1: 476-489.  

·  Guo-Chao Chu#; Man Pan#; Jiabin Li#; Sanling Liu; Chong Zuo; Ze-Bin Tong; Jing-Si Bai; Qingyue Gong; Huasong Ai; Jian Fan; Xianbin Meng; Yi-Chao Huang; Jing Shi; Haiteng Deng; Changlin Tian*; Yi-Ming Li*; Lei Liu*; Cysteine-Aminoethylation-Assisted Chemical Ubiquitination of Recombinant Histones, Journal of the American Chemical Society, 2019, 141: 3654-3663.  

·  Qian Qu#; Man Pan#; Shuai Gao; Qing-Yun Zheng; Yuan-Yuan Yu; Jia-Can Su; Xiang Li; Hong-Gang Hu*; A Highly Efficient Synthesis of Polyubiquitin Chains, Advanced Science, 2018, 5: 1800234.

·  Man Pan#; Shuai Gao#; Yong Zheng#; Xiaodan Tan; Huan Lan; Xianglong Tan; Demeng Sun; Lining Lu; Tian Wang; Qingyun Zheng; Yichao Huang; Jiawei Wang; Lei Liu*; Quasi-Racemic X-ray Structures of K27-Linked Ubiquitin Chains Prepared by Total Chemical Synthesis, Journal of the American Chemical Society, 2016, 23(138): 7429-7435. 

·   Man Pan#; Yao He#; Ming Wen; Fangming Wu; Demeng Sun; Sijian Li; Longhua Zhang; Yiming Li; Changlin Tian*; One-pot hydrazide-based native chemical ligation for efficient chemical synthesis and structure determination of toxin Mambalgin-1, Chemical Communications, 2014, 50(44): 5839.   

·  Yi-Ming Li; Yi-Tong Li; Man Pan; Xiu-Qi Kong; Yi-Chao Huang; Zhang-Yong Hong; Lei Liu*; Irreversible site-specific hydrazinolysis of proteins by use of Sortase, Angewandte Chemie International Edition, 2014, 53(8): 2202.   

·   Yichao Huang; Yiming Li; Yang Chen; Man Pan; Yitong Li; Li Yu; Qingxiang Guo; Lei Liu*; Synthesis of Autophagosomal Marker Protein LC3-II under Detergent-Free Conditions, Angewandte Chemie International Edition, 2013, 52(18): 4858-4862. 

二. 蛋白工具开发：

·  Qingyun Zheng^#; Zhen Su^#; Yuanyuan Yu*; Lei Liu*; Recent progress in dissecting ubiquitin signals with chemical biology tools. Current Opinion Chemical Biology, 2022, 102187.

·  Tian Wang^#, Chuntong Li^#, Meijing Wang^#, Jiachen Zhang, Qingyun Zheng, Lujun Liang, Guochao Chu, Xiaolin Tian, Haiteng Deng, Wei He*, Lei Liu*, Jinghong Li*. Expedient synthesis of ubiquitin-like protein ISG15 tools through chemo-enzymatic ligation catalyzed by a viral protease Lbpro. Angewandte Chemie International Edition, 2022, e202206205. 

·  Yuanyuan Yu^#; Qingyun Zheng^#; Satchal K. Erramilli^#; Man Pan^#*; Seongjin Park; Yuan Xie; Jingxian Li; Jingyi Fei; Anthony A. Kossiakoff＊; Lei Liu＊; Minglei Zhao＊; K29-Linked Ubiquitin Signaling Regulates Proteotoxic Stress Response and Cell Cycle, Nature Chemical Biology, 2021, 17: 896–905.

·  Qingyun Zheng^#; Tian Wang^#; Guochao Chu^#; Chong Zuo; Rui Zhao; Xin Sui; Linzhi Ye; Yuanyuan Yu; Jingnan Chen; Xiangwei Wu; Wenhao Zhang; Haiteng Deng; Jing Shi; Man Pan*; Yiming Li*; Lei Liu*; An E1-Catalyzed Chemoenzymatic Strategy to Isopeptide-N-Ethylated Deubiquitylase-Resistant Ubiquitin Probes, Angewandte Chemie International Edition, 2020, 59(32): 13496-13501.  

·  Man Pan^#; Qingyun Zheng^#; Shan Ding^#; Lujia Zhang^#; Qian Qu; Tian Wang; Danning Hong; Yujing Ren; Lujun Liang; Chunlai Chen*; Ziqing Mei*; Lei Liu*; Chemical Protein Synthesis Enabled Mechanistic Studies on the Molecular Recognition of K27-linked Ubiquitin Chains, Angewandte Chemie International Edition, 2019, 9(58): 2627-2631. 

·  Man Pan^#; Shan Li^#; Xiang Li; Feng Shao; Lei Liu*; Honggang Hu*; Synthesis of and specific antibody generation for glycopeptides with arginine N-GlcNAcylation, Angewandte Chemie International Edition, 2014, 53(52): 14517-21. 

·  Xiang-Long Tan^#; Man Pan^#; Yong Zheng; Shuai Gao; Lu-Jun Liang; Yi-Ming Li*; Sortase-mediated chemical protein synthesis reveals the bidentate binding of bisphosphory-lated p62 with K63 diubiquitin, Chemical Science, 2017, 8: 6881-6887.  

·  Xiao-Dan Tan^#; Man Pan^#; Shuai Gao; Yong Zheng; Jing Shi; Yi-Ming Li*; A diubiquitin-based photoaffinity probe for profiling K27-linkage targeting deubiquitinases,  Chemical Communications, 2017, 53: 10208-10211.

三.蛋白工具应用：

·  Man Pan^#＊; Qingyun Zheng^#; Tian Wang^#; Lujun Liang^#; Chong Zuo; Ruichao Ding; Huasong Ai; Yuan Xie; Si Dong; Yuanyuan Yu*; Lei Liu*; Minglei Zhao＊; Structural Insights Into the Initiation and Elongation of Ubiquitination by Ubr1, Nature, 2021, 600, 334–338.

·  Man Pan^#＊; Yuanyuan Yu^#; Huasong Ai; Qingyun Zheng; Yuan Xie; Lei Liu^*; Minglei Zhao*; Mechanistic insight into substrate processing and allosteric inhibition of human p97, Nature Structural & Molecular Biology, 2021, 28, 614-625.

·  Man Pan^#; Qingyun Zheng^#; Yuanyuan Yu^#; Huasong Ai; Yuan Xie; Xin Zeng; Chu Wang; Lei Liu*; Minglei Zhao*; Seesaw conformations of Npl4 in the human p97 complex and the inhibitory mechanism of a disulfiram derivative, Nature Communications, 2021, 12(1): 121.    

·  Shuai Gao^#; Man Pan^#; Yong Zheng^#; Yichao Huang; Qingyun Zheng; Demeng Sun; Lining Lu; Xiaodan Tan; Xianglong Tan; Huan Lan; Jiaxing Wang; Tian Wang; Jiawei Wang*; Lei Liu*; Monomer/Oligomer Quasi-Racemic Protein Crystallography, Journal of the American Chemical Society, 2016, 43(138): 14497-14502. 

·  Demeng Sun^#; You Yu^#; Xiaobin Xue^#; Man Pan^#; Ming Wen; Siyu Li; Qian Qu; Xiaorun Li; Longhua Zhang; Xueming Li; Lei Liu*; Maojun Yang*; Changlin Tian*; Cryo-EM structure of the ASIC1a–mambalgin-1 complex reveals that the peptide toxin mambalgin-1 inhibits acid-sensing ion channels through an unusual allosteric effect, Cell Discovery, 2018, 4(1): 27. 

·  Yanan Dong#; Yajuan Mu; Yongchao Xie; Yupeng Zhang; Youyou Han; Yu Zhou; Wenhe Wang; Zihe Liu; Mei Wu; Hao Wang; Man Pan; Ning Xu; Cong-Qiao Xu; Maojun Yang; Shilong Fan; Haiteng Deng; Tianwei Tan; Xiaoyun Liu; Lei Liu; Jun Li; Jiawei Wang; Xianyang Fang; Yue Feng*; Structural basis of ubiquitin modification by the Legionella effector SdeA, Nature, 2018, 557(7707): 674-678. 

·  Jingxian Li^#; Yuan Xie; Shaleeka Cornelius; Xian Jiang; Richard Sando; Szymon P. Kordon; Man Pan; Katherine Leon; Thomas C. Südhof; Minglei Zhao*; Demet Arac*; Alternative splicing controls teneurin-latrophilin interaction and synapse specificity by a shape-shifting mechanism, Nature Communications, 2020, 11(1): 2140. 

·  Yi-Tong Li^#; Cong Yi; Chen-Chen Chen; Huan Lan; Man Pan; Shao-Jin Zhang; Yi-Chao Huang; Chao-Jian Guan; Yi-Ming Li*; Li Yu*; Lei Liu*; A semisynthetic Atg3 reveals that acetylation promotes Atg3 membrane binding and Atg8 lipidation, Nature Communications, 2017, 8(8): 14846.  

·  Demeng Sun^#; Sanling Liu; Siyu Li; Mengge Zhang; Fan Yang; Ming Wen; Pan Shi; Tao Wang; Man Pan; Shenghai Chang; Xing Zhang; Longhua Zhang＊; Changlin Tian*; Lei Liu*; Structural insights into human acid-sensing ion channel 1a inhibition by snake toxin mambalgin1, eLife, 2020, 9: e57096.