2024年：

Chen S, Pei CX, Xu S, Li H, Liu YS, Wang Y, Jin C, Dean N, Gao XD*. Rft1 catalyzes lipid-linked oligosaccharide translocation across the ER membrane. Nat Commun. 2024 Jun 17;15(1):5157. doi: 10.1038/s41467-024-48999-3. PMID: 38886340.

Tang YH, Leng JX, Yang G, Gao XD, Liu YS, Fujita M. Production of CA125 with Tn antigens using a glycosylphosphatidylinositol anchoring system. J Biochem. 2024 Jul 1;176(1):23-34. doi: 10.1093/jb/mvae019. PMID: 38382634.

Wang B, Wang Y, Zhou X, Gao XD, Fujita M, Li Z. Highly efficient expression of Rasamsonia emersonii lipase in Pichia pastoris: characterization and gastrointestinal simulated digestion in vitro. J Sci Food Agric. 2024 Jul;104(9):5603-5613. doi: 10.1002/jsfa.13390. Epub 2024 Mar 4. PMID: 38363126.

Zhao JM, Wang YK, Shi BW, Wang YX, Jiang YF, Yang GL, Gao XD*, Qiang T. Microwave biosensor for the detection of growth inhibition of human liver cancer cells at different concentrations of chemotherapeutic drug. Front Bioeng Biotechnol. 2024 May 13;12:1398189. doi: 10.3389/fbioe.2024.1398189. PMID: 38803847.

Wang YK, Shi BW, Zhao JM, Wang YX, Jiang YF, Yang GL, Gao XD*, Qiang T. Highly Sensitive and Linear Resonator-Based Biosensor for White Blood Cell Counting: Feasible Measurement Method and Intrinsic Mechanism Exploration. Biosensors (Basel). 2024 Apr 7;14(4):180. doi: 10.3390/bios14040180. PMID: 38667173.

Chen J, Wen P, Tang YH, Li H, Wang Z, Wang X, Zhou X, Gao XD, Fujita M, Yang G. Proteome and Glycoproteome Analyses Reveal Regulation of Protein Glycosylation Site-Specific Occupancy and Lysosomal Hydrolase Maturation by N-Glycan-Dependent ER-Quality Control. J Proteome Res. 2024 Sep 5. doi: 10.1021/acs.jproteome.4c00378. Epub ahead of print. PMID: 39235835.

Zhou X, Wang X, Bai R, Li H, Hua D, Gao XD, Yang G, Liu Q. Protein Signatures for Distinguishing Colorectal Cancer Liver Metastases from Primary Liver Cancer Using Tissue Slide Proteomics. Front Biosci (Landmark Ed). 2024 Jan 9;29(1):3. doi: 10.31083/j.fbl2901003. PMID: 38287800.

2023年：

Wang X, Li H, Wang Z, Chen J, Chen W, Zhou X, Zhang L, Xu S, Gao XD*, Yang G. Site- and Structure-Specific Glycosylation Signatures of Bovine, Caprine, Porcine, and Human Milk-Derived Extracellular Vesicles. J Agric Food Chem. 2023 Dec 27;71(51):20826-20837. doi: 10.1021/acs.jafc.3c06439. Epub 2023 Dec 14. PMID: 38096130.

Li S, LI X, Meng J, Bao W, Wang S, Ye P, Gao X-D*.  Wei W*. Dynamic shielding of bacterial outer membrane vesicles for safe and efficient chemo-immunotherapy against tumors. Nano Research 2023 Nov

Xiang MH, Lu TT, Gao XD, Wang N. Efficient production and characterization of soluble active human β-1,2-N-acetylglucosaminyltransferase II in bacteria. J Biosci Bioeng. 2023 Sep;136(3):166-172. doi: 10.1016/j.jbiosc.2023.06.005.

Wang Y, Wang B, Gao Y, Nakanishi H, Gao XD, Li Z. Highly efficient expression and secretion of human lysozyme using multiple strategies in Pichia pastoris. Biotechnol J. 2023 Jul 20:e2300259. doi: 10.1002/biot.202300259.

Cao SY, Liu YS, Gao XD, Kinoshita T, Fujita M. A lipid scramblase TMEM41B is involved in the processing and transport of GPI-anchored proteins. J Biochem. 2023 Jun 5:mvad041. doi: 10.1093/jb/mvad041.

Jia JX, Peng SL, Kalisa NY, Chao Q, Zhou Z, Gao XD*, Wang N*. A liposomal carbohydrate vaccine, adjuvanted with an NKT cell agonist, induces rapid and enhanced immune responses and antibody class switching. J Nanobiotechnology. 2023 Jun 2;21(1):175. doi: 10.1186/s12951-023-01927-x.

Wang H, Gao XD*, Yue H*. Lentinan-laden microspheres reprogram the tumor microenvironment and improve anti-PD-L1 efficacy. J Mater Chem B. 2023 May 24;11(20):4475-4484. doi: 10.1039/d3tb00047h.

Liu YS, Wang Y, Zhou X, Zhang L, Yang G, Gao XD, Murakami Y, Fujita M, Kinoshita T. Accumulated precursors of specific GPI-anchored proteins upregulate GPI biosynthesis with ARV1. J Cell Biol. 2023 May 1;222(5):e202208159. doi: 10.1083/jcb.202208159.

Chi K, Xu H, Li H, Yang G, Zhou X*, Gao XD*. Expression of a Siglec-Fc Protein and Its Characterization. Biology (Basel). 2023 Apr 10;12(4):574. doi: 10.3390/biology12040574.

Yang G, Zuo C, Lin Y, Zhou X, Wen P, Zhang C, Xiao H, Jiang M, Fujita M, Gao XD*, Fu F*. Comprehensive proteome, phosphoproteome and kinome characterization of luminal A breast cancer. Front Oncol. 2023 Mar 31;13:1127446. doi: 10.3389/fonc.2023.1127446.

Yang Y, Yang G, Li ZJ, Liu YS, Gao XD*, Nakanishi H*. Studies on the Proteinaceous Structure Present on the Surface of the Saccharomyces cerevisiae Spore Wall. J Fungi (Basel). 2023 Mar 23;9(4):392. doi: 10.3390/jof9040392.

Xu HL, Zhou X, Chen S, Xu S, Li Z*, Nakanishi H*, Gao XD*. Rare sugar L-sorbose exerts antitumor activity by impairing glucose metabolism. Commun Biol. 2023 Mar 11;6(1):259. doi: 10.1038/s42003-023-04638-z. 

2022年：

Wang CD, Xu S, Chen S, Chen ZH, Dean N, Wang N*, Gao X-D*. An in vitro assay for enzymatic studies on human ALG13/14 heterodimeric UDP-N-acetylglucosamine transferase. Front Cell Dev Biol. 2022 Sep 19;10:1008078. doi: 10.3389/fcell.2022.1008078.

Cao Q, Li T, Jia J-X, Li Z, Peng P, Gao X-D*, Wang N*. Spore-Encapsulating Glycosyltransferase Catalysis Tandem Reactions: Facile Chemoenzymatic Synthesis of Complex Human Glycans. ACS Catalysis, 2022, 12, 3181-3188.

Yang Y, Liu G, Li F, Carey LB, Sun C, Ling K, Tachikawa H, Fujita M, Gao XD*, Nakanishi H*. Receptor for advanced glycation end-products (RAGE) mediates phagocytosis in nonprofessional phagocytes. Commun Biol. 2022 Aug 16;5(1):824. doi: 10.1038/s42003-022-03791-1.

Liu G, Yang Y, Yang G, Duan S, Yuan P, Zhang S, Li F, Gao XD*, Nakanishi H*. Triosephosphate Isomerase and Its Product Glyceraldehyde-3-Phosphate Are Involved in the Regulatory Mechanism That Suppresses Exit from the Quiescent State in Yeast Cells.m Microbiol Spectr. 2022 Aug 4:e0089722. doi: 10.1128/spectrum.00897-22.

Li Z, Feng L, Chen Z, Hu Y, Fei K, Xu H, Gao XD*. Efficient enzymatic synthesis of D-allulose using a novel D-allulose-3-epimerase from Caballeronia insecticola. J Sci Food Agric. 2022 Jul 24. doi: 10.1002/jsfa.12147. PMID: 35871484.

Li Z, Li W, Wang Y, Chen Z, Nakanishi H, Xu X, Gao XD*. Establishment of a Novel Cell Surface Display Platform Based on Natural "Chitosan Beads" of Yeast Spores. J Agric Food Chem. 2022 Jun 22;70(24):7479-7489. doi: 10.1021/acs.jafc.2c01983. Epub 2022 Jun 9. PMID: 35678723

Chen Z, Gao XD, Li Z. Recent Advances Regarding the Physiological Functions and Biosynthesis of D-Allulose. Front Microbiol. 2022 Apr 14;13:881037. doi: 10.3389/fmicb.2022.881037. eCollection 2022. PMID: 35495640

Wang N, Li ST, Xiang MH, Gao XD*. Alg mannosyltransferases: From functional and structural analyses to the lipid-linked oligosaccharide pathway reconstitution. Biochim Biophys Acta Gen Subj. 2022 May;1866(5):130112. doi: 10.1016/j.bbagen.2022.130112. Epub 2022 Feb 22. PMID: 35217128

Xiang MH, Xu XX, Wang CD, Chen S, Xu S, Xu XY, Dean N, Wang N*, Gao XD. Topological and enzymatic analysis of human Alg2 mannosyltransferase reveals its role in lipid-linked oligosaccharide biosynthetic pathway. Commun Biol. 2022 Feb 8;5(1):117. doi: 10.1038/s42003-022-03066-9.

Chen Z, Fei K, Hu Y, Xu X, Gao XD, Li Z. Identification of a Novel Alditol Oxidase from Thermopolyspora flexuosa with Potential Application in D-Glyceric Acid Production. Mol Biotechnol. 2022 Feb 7. doi: 10.1007/s12033-022-00459-3.

Yuan L, Gao XD, Xia Y. Optimising the oil phases of aluminium hydrogel-stabilised emulsions for stable, safe and efficient vaccine adjuvant. Front Chem Sci Eng. 2022 Jan 17:1-12. doi: 10.1007/s11705-021-2123-1.

2021年：

Yoko-O T, Komatsuzaki A, Yoshihara E, Zhao S, Umemura M, Gao X-D, Chiba Y. Regulation of alcohol oxidase gene expression in methylotrophic yeast Ogataea minuta. J Biosci Bioeng. 2021 Nov;132(5):437-444. doi:10.1016/j.jbiosc.2021.08.001.

Liu SS, Jin F, Liu YS, Murakami Y, Sugita Y, Kato T, Gao XD, Kinoshita T, Hattori M, Fujita M. Functional Analysis of the GPI Transamidase Complex by Screening for Amino Acid Mutations in Each Subunit. Molecules. 2021 Sep 8;26(18):5462. doi: 10.3390/molecules26185462.

Nakamura TS, Suda Y, Muneshige K, Fujieda Y, Okumura Y, Inoue I, Tanaka T, Takahashi T, Nakanishi H, Gao XD, Okada Y, Neiman AM, Tachikawa. H.Suppression of Vps13 adaptor protein mutants reveals a central role for PI4P in regulating prospore membrane extension. PLoS Genet. 2021 Aug 18;17(8):e1009727. doi: 10.1371/journal.pgen.1009727.

Liu SS, Liu YS, Guo XY, Murakami Y, Yang G, Gao XD, Kinoshita T, Fujita M.

 A knockout cell library of GPI biosynthetic genes for functional studies of GPI-anchored proteins. Commun Biol. 2021 Jun 23; 4(1): 777. doi: 10.1038/s42003-021-02337-1.

Guo XY. Gao X-D. Fujita M. Sulfation of a FLAG tag mediated by SLC35B2 and TPST2 affects antibody recognition. PLoS One. 2021 May 5; 16(5):e0250805. doi: 10.1371/journal.pone.0250805. eCollection 2021.

Leng JX, Ren WW, Li Y, Yang G, Gao XD, Fujita M. Cell engineering for the production of hybrid-type N-glycans in HEK293 cells. J Biochem. 2021 Apr 20:mvab051. doi: 10.1093/jb/mvab051.

Yang J, Hirata T, Liu YS, Guo XY, Gao XD, Kinoshita T, Fujita M. Human SND2 mediates ER targeting of GPI-anchored proteins with low hydrophobic GPI-attachment signals. FEBS Lett. 2021 Apr 10. doi: 10.1002/1873-3468.14083.

Li A, Liu J, Ding F, Wu X, Pan C, Wang Q, Gao M, Duan S, Han X, Xia K, Liu S, Wu Y, Zhou Z, Zhang X, Gao X-D*. Maca extracts regulate glucose and lipid metabolism in insulin-resistant HepG2 cells via the PI3K/AKT signalling pathway. Food Sci Nutr. 2021 Mar 29;9(6):2894-2907. doi: 10.1002/fsn3.2246. eCollection 2021 Jun.

Huang YF, Aoki K, Akase S, Ishihara M, Liu YS, Yang G, Kizuka Y, Mizumoto S, Tiemeyer M, Gao XD, Aoki-Kinoshita KF, Fujita M. Global mapping of glycosylation pathways in human-derived cells. Dev Cell. 2021 Mar 9:S1534-5807(21)00163-5. doi: 10.1016/j.devcel.2021.02.023

2020年：

Li F, Yang G, Tachikawa H, Shao K, Yang Y, Gao X-D*, Nakanishi H*. Identification of novel O-GlcNAc transferase substrates using yeast cells expressing OGT. J Gen Appl Microbiol. 2020 Nov 21. doi: 10.2323/jgam.2020.04.002.

Liu YS, Matabaro E, Gao XD, Fujita M. Selecting cells expressing high levels of recombinant proteins using the GPI-anchored protein with selenocysteine system. J Biosci Bioeng. 2020 Nov 3:S1389-1723(20)30394-7.

doi: 10.1016/j.jbiosc.2020.10.004

Jia JX, Kalisa NY, Lu TT, Zhou Z, Gao X-D*, Wang N*. Chemo-enzymatic synthesis of the ALG1-CDG biomarker and evaluation of its immunogenicity. Bioorg Med Chem Lett. 2020 Oct 17;30(24):127614. doi: 10.1016/j.bmcl.2020.127614.

Peng S, Cao F, Xia Y, Gao XD, Dai L, Yan J, Ma G. Particulate Alum via Pickering Emulsion for an Enhanced COVID-19 Vaccine Adjuvant. Adv Mater. 2020 Oct;32(40):e2004210. doi: 10.1002/adma.202004210.

Liu G, Gao X-D*, Nakanishi H*, Unique Properties of the S. cerevisiae Spore Wall and Its Applications. Trends in Glycoscience and Glycotechnology. 2020 Volume 32, Issue 190, Pages E189-E193

Guo XY, Liu YS, Gao XD, Kinoshita T, Fujita M*. Calnexin mediates the maturation of GPI-anchors through ER retention. J Biol Chem. 2020 Sep 23:jbc.RA120.015577. doi: 10.1074/jbc.RA120.015577.

Li Z, Liu X, Nakanishi H, Gao X-D*. Encapsulation of Mannose-6-phosphate Isomerase in Yeast Spores and Its Application in L-Ribose Production. J Agric Food Chem. 2020 Jun 24;68(25):6892-6899. doi: 10.1021/acs.jafc.0c02399.

Chao Q, Ding Y, Chen Z-H, Xiang M-H, Wang N* and Gao X-D*. (2020) Recent Progress in Chemo-Enzymatic Methods for the Synthesis of N-Glycans. Front. Chem. 2020 Jun 16:8:513. doi: 10.3389/fchem.2020.00513

Zhao SB, Dean N, Gao XD, Fujita M*. MON2 guides Wntless transport to the Golgi through recycling endosomes. Cell Struct Funct. 2020 Jun 13;45(1):77-92. doi: 10.1247/csf.20012.

Shao K, Li F, Yang Y, Wang N, Gao X-D*, Nakanishi H*. Characteristics of SNARE proteins are defined by distinctive properties of SNARE motifs. Biochim Biophys Acta Gen Subj. 2020 Jun 6;1864(10):129658. doi: 10.1016/j.bbagen.2020.129658.

Basiony M, Yang Y, Liu G, Gao X-D*, Nakanishi H*. Studies on the Properties of the Sporulation Specific Protein Dit1 and its Product Formyl Tyrosine. J Fungi (Basel). 2020 Jun 3;6(2):E77. doi: 10.3390/jof6020077.

Jin Y, Okamoto M, Chibana H, Liu G, Gao X-D*, Nakanishi H*. Functional characteristics of Svl3 and Pam1 that are required for proper cell wall formation in yeast cells. Yeast. 2020 Jun 3. doi: 10.1002/yea.3502.

Chen Z, Li Z, Li F, Wang N, Gao X-D*. Characterization of alditol oxidase from Streptomyces coelicolor and its application in the production of rare sugars. Bioorg Med Chem. 2020 May 15;28(10):115464. doi: 10.1016/j.bmc.2020.115464.

Li ZJ*, Li F, Cai L, Chen Z, Qin L, Gao X-D*. One-pot multi-enzyme synthesis of rare ketoses from glycerol. J Agric Food Chem. 2020 Feb 5;68(5):1347-1353. doi: 10.1021/acs.jafc.9b06748.

2019年：

Chen Z, Li ZJ*, Li F, Wang M, Y Gao, Wang N, Gao X-D*. Cascade synthesis of rare ketoses by whole cells based on L-rhamnulose-1-phosphate aldolase. Enzyme and Microbial Technology, 2019 Oct. DOI: 10.1016/j.enzmictec.2019.109456.

Du Y, Xia Y, Zou Y, Hu Y, Fu J, Wu J, Gao X-D*, Ma G*. Exploiting the Lymph-Node-Amplifying Effect for Potent Systemic and Gastrointestinal Immune Responses via Polymer/Lipid Nanoparticles. ACS Nano. 2019 Oct 21. 13, 13809−13817

doi: 10.1021/acsnano.9b04071.

Li A, Duan S, Dang Y, Zhang X, Xia K, Liu S, Han X, Wen J, Li Z, Wang X, Liu J, Yuan P, Gao X-D*. Origin identification of Chinese Maca using electronic nose coupled with GC-MS. Sci Rep. 2019 Aug 21;9(1):12216. doi: 10.1038/s41598-019-47571-0.

Shao K, Wang Q, Wang N, Gao X-D*, Nakanishi H*. Construction of functional chimeras of syntaxin-1A and its yeast orthologue, and their application to the yeast cell-based assay for botulinum neurotoxin serotype C. Biochim Biophys Acta Gen Subj. 2019 Jul 11. pii: S0304-4165(19)30176-X. doi: 10.1016/j.bbagen.2019.07.005.

Rao J, Zhang R, Liang H, Gao XD, Nakanishi H, Xu Y*. Efficient chiral synthesis by Saccharomyces cerevisiae spore encapsulation of Candida parapsilosis Glu228Ser/(S)-carbonyl reductase II and Bacillus sp. YX-1 glucose dehydrogenase in organic solvents. Microb Cell Fact. 2019 May 20;18(1):87. doi: 10.1186/s12934-019-1137-6.

Ren WW, Jin ZC, Dong W, Kitajima T, Gao X-D*, Fujita M*. Glycoengineering of HEK293 cells to produce high-mannose-type N-glycan structures. J Biochem. 2019 May 7. pii: mvz032. doi: 10.1093/jb/mvz032.

Mensah EO, Guo XY, Gao XD, Fujita M*. Establishment of DHFR-deficient HEK293 cells for high yield of therapeutic glycoproteins. J Biosci Bioeng. 2019 Apr 25. pii: S1389-1723(19)30081-7. doi: 10.1016/j.jbiosc.2019.04.005.

Li ST, Lu TT, Xu XX, Ding Y, Li Z, Kitajima T, Dean N, Wang N*, Gao X-D*. Reconstitution of the lipid-linked oligosaccharide pathway for assembly of high-mannose N-glycans. Nat Commun. 2019 Apr 18;10(1):1813. doi: 10.1038/s41467-019-09752-3.

Liu X, Li Z, Chen Z, Wang N, Gao Y, Nakanishi H*, Gao X-D*. Production of l-Ribulose Using an Encapsulated l-Arabinose Isomerase in Yeast Spores. J Agric Food Chem. 2019 Apr 16. doi: 10.1021/acs.jafc.9b00640.

Zhao SB, Suda Y, Nakanishi H, Wang N, Yoko-O T, Gao X-D*, Fujita M*. Yeast Dop1 is required for glycosyltransferase retrieval from the trans-Golgi network. BBA-general subjects. 2019 Apr 11. pii: S0304-4165(19)30086-8. doi: 10.1016/j.bbagen.2019.04.009.

Ren W, Jin Z, Dong W, Kitajima T, Gao XD, Fujita M. Glycoengineering of HEK293 cells to produce high-mannose-type N-glycan structures. Journal of Biochemistry (Tokyo) (2019). 166(3) 245-258. DOI: 10.1093/jb/mvz032

Feng S, Zhang H, Xu S, Zhi C, Nakanishi H, Gao X-D*. Folate-conjugated, mesoporous silica functionalized boron nitride nanospheres for targeted delivery of doxorubicin. Mater Sci Eng C Mater Biol Appl. 2019 Mar;96:552-560. doi: 10.1016/j.msec.2018.11.063. PMID: 30606565