项目研究员，博士生导师，中国科学院择优人才。主要研究方向是基于高分辨率质谱的复杂样品和单细胞的蛋白质糖基化修饰分析新技术的开发和应用；开发了组织，血液，外泌体和类器官等多种样品的糖组学和糖蛋白质组学的深度分析技术，实现了用于肿瘤微环境和复杂组织的单细胞蛋白质组和糖蛋白质组分析技术突破。在Analytical Chemistry，Journal of Agricultural and Food Chemistry等期刊发表论文60余篇，其中第一作者或通讯作者文章20余篇；申请发明授权发明专利15项。主持参与国家自然科学基金（面上、青年）等科研项目，获省级科学技术奖一等奖1项，国家和省级人才项目各1项。

发表文章：

1. X.-Y. Wang, H.-J., Li, Z.-B. Wang, J.-R. Chen, W.-Y. Chen, X.-M. Zhou, L.-N. Zhang, S.-Q. Xu, X.-D. Gao,* G.-L.Yang*. Site- and structure-specific glycosylation signatures of bovine, caprine, porcine, and human milk-derived extracellular vesicles. Journal of Agricultural and Food Chemistry. 71, 20826−20837(2023)

2. G.-L.Yang#, C.-Y. Zuo#, Y.-X. Lin#, X.-M. Zhou, P.-P. Wen, C.-R. Zhang, H. Xiao, M.-C. Jiang, M. Fujita, X.-D. Gao*, F.-M. Fu*. Comprehensive proteome, phosphoproteome and kinome characterization of luminal A breast cancer. Frontiers in Oncology. 13:1127446(2023)

3. X.-M. Zhou, K.-J. Chi, C.-R. Zhang, Q. Liu, G.-L.Yang*, Sialylation: A cloak for tumors to trick the immune system in the microenvironment. Biology. 12(6):832(2023)

4. P.-P. Wen, J.-R. Chen, C.-Y. Zuo, X.-D. Gao, M. Fujita*, G.-L.Yang*, Proteome and glycoproteome analyses reveal the protein N-linked glycosylation specificity of STT3A and STT3B. Cells. 11, 2775(2022)

5. G.-L.Yang*, H. Zhang, W. Yi, S. Yan, L.-W. Cao. Editorial: Protein Glycosylation-Advances in Identification, Characterization and Biological Function Elucidation Using Mass Spectrometry. Frontiers in Chemistry. 10(847242):1-3 (2022)

6. G.-L. Yang*, Q. Wang, L.-J. Chen, M. Betenbaugh, H. Zhang, Glycoproteomic characterization of FUT8 knock-out CHO cells reveals roles of FUT8 in the glycosylation. Frontiers in Chemistry. doi.org/10.3389/fchem.2021.755238 (2021)

7. N.-N Bi, S. Zhao, J.-F. Zhang, Y. Cheng, C.-Y. Zuo, G.-L.Yang*, K. Yang*, Proteomics investigations of potential protein biomarkers in sera of rabbits infected with schistosoma japonicum. Frontiers in Cellular and Infection Microbiology. doi: 10.3389/fcimb.2021.784279 (2021)

8. W.-Y. Chen, R. Wang, D. Li, C.-Y. Zuo, P.-P. Wen, H.-L. Liu, Y.-Q. Chen, M. Fujita, Z.-M. Wu*, G.-L. Yang*, Comprehensive analysis of the glycome and glycoproteome of bovine milk-derived exosomes. Journal of Agricultural and Food Chemistry. 68(45): 12692–12701(2020)

9. G.-L. Yang, N. Höti, S.-Y. Chen., Y.-Y. Zhou, Q. Wang, M. Betenbaugh, H. Zhang*, One-step enrichment of intact glycopeptides from glycoengineered chinese hamster ovary cells. Frontiers in Chemistry. doi.org/10.3389/ fchem.2020.00240 (2020)

10. X.-M. Zhou, G.-L. Yang*, F. Guan*, Biological functions and analytical strategies of sialic acids in tumor. Cells. 9 (2): 273 (2020)

11. G.-L. Yang, Y.-W. Hu, S.-S. Sun, C.-Z. Ouyang, W.-M. Yang, Q. Wang, M. Betenbaugh, H. Zhang*, Comprehensive Glycoproteomic Analysis of Chinese Hamster Ovary Cells. Analytical Chemistry. 90(24): 14294−14302 (2018)

12. G.-L. Yang, L.-Y. Huang, J.-X. Zhang, H.-J. Yu, Z. Li, F. Guan*, Global Identification and Differential Distribution Analysis of Glycans in Subcellular Fractions of Bladder Cells. International Journal of Biological Sciences. 12(7): 799–811(2016)

13. G.-L. Yang, W. Lu, D. Yu, C.-W. Sun, J. Guo, Z. Li, F. Guan*, Quantitative analysis of differential proteome expression in epithelial-to-mesenchymal transition of bladder epithelial cells using SILAC method, Molecules. 21(84) doi:10.3390/molecules21010084 (2016)

14. G.-L. Yang, Z.-Q. Tan, W. Lu, J. Guo, H.-J. Yu, J.-M.Yu, C.-W. Sun, X.-W. Qi, Z. Li*, F. Guan*, Quantitative glycome analysis of N‑glycan patterns in bladder cancer vs normal bladder cells using an integrated strategy. Journal of  Proteome Research. 14: 639−653 (2015)

15.G.-L. Yang, Z.-P, Xu, W.Lu, X. Li, C. Sun, J. Guo, P. Xue*, F.Guan*,  Quantitative analysis of differential proteome expression in bladder cancer vs. normal bladder cells using SILAC method. PLoS ONE. 10(7): e0134727 (2015)

16.G.-L. Yang, T. Cui, Y. Wang, S.-S. Sun, T.-R. Ma, T. Wang, Q.-L. Chen, Z. Li*,Selective isolation and analysis of glycoprotein fractions and their glycomes  from hepatocellular carcinoma sera, Proteomics. 13(9): 1481-1498 (2013)

17.G.-L. Yang, W. Chu, H. Zhang, X.-X. Sun, T.-X. Cai, L.-Y. Dang, Q.-Z. Wang, H.-J. Yu, Y.-G. Zhong, Z. Chen, F.-Q. Yang, Z. Li*, Isolation and identification of mannose-binding proteins and estimation of their abundance in hepatocellular carcinoma sera, Proteomics. 13(5): 878-892 (2013)

18.G.-L.Yang, T. Cui, Q.-L. Chen, T.-R. Ma, Z. Li, Isolation and identification of native membrane glycoproteins from living cell by concanavalin A–magnetic particle conjugates, Analytical Biochemistry, 421:339-341 (2012)

19.G.-L.Yang, J.-X. Zhang, C.-M. Liu, F. Guan*, Quantitative analysis of N-glycome of different bladder cancer cells, Progress in Biochemistry and Biophysics 42(12):1144-1154 (2015)

20.G.-L.Yang, T.-R. Ma, Z. Li*, Enrichment and characterization of total N-linked glycans from glycoproteins by ultrafiltration units and mass spectrometry, Progress in Biochemistry and Biophysics, 41(4):403-408 (2014)

21. Y. Yang, G.-L. Yang, Z.-J. Li, Y.-S. Liu, X.-D. Gao, H. Nakanishi, Studies on the Proteinaceous Structure Present on the Surface of the Saccharomyces cerevisiae Spore Wall. Journal of Fungi. 9(4):392(2023)

22. Y.-S. Liu, Y.-C. Wang, X.-M. Zhou, L Zhang, G.-L. Yang, X.-D. Gao, Y. Murakami, M. Fujita. Accumulated precursors of specific GPI-anchored proteins upregulate GPI biosynthesis with ARV1. Journal of Cell Biology 222 (5), e202208159(2023)

23.R. Wang, P.-P. Wen, G.-L. Yang, Y.-Y. Feng, Y.-Y. Mi, X.-Y. Wang, S.-L. Zhu, Y.-Q. Chen, N-glycosylation of GDF15 abolishes its inhibitory effect on EGFR in AR inhibitor-resistant prostate cancer cells, Cell Death and Disease, 13(626): (2022)

24.G.-Y. Liu, Y. Yang, G.-L. Yang, S.-L. Duan, P. Yuan, S. Zhang, F. Li, X.-D. Gao, H. Nakanishi, Triosephosphate isomerase and its product glyceraldehyde-3-phosphate are involved in the regulatory mechanism that suppresses exit from the quiescent state in yeast cells, Microbiology Spectrum, 10(4): 1-18 (2022)

25.Q. Wang, T.-X. Wang, W.-W. Wu, C.-Y. Lin, S. Yang, G.-L. Yang, E. Jankowska, Y.-F. Hu, R-F Shen, M. J. Betenbaugh, and J. F. Cipollo, Comprehensive N- and O- glycoproteomic analysis of multiple Chinese hamster ovary host cell lines, Journal of Proteome Research, 21, 2341−2355 (2022)

26.S. Yan*, A. Hodžić, G. Bandini, G.-L. Yang, J. Vanbeselaere and Y Guérardel. Editorial: Characterization, Biosynthesis and Biological Functions of Novel Glyco-EpitopesFrontoers in Molecular Biosciences 9(871037):1-3 (2022)

27.Y.-F. Huang, K. Aoki, S. Akase, M. Ishihara, Y.-S. Liu, G.-L. Yang, Y Kizuka, S. Mizumoto, M. Tiemeyer, X.-D. Gao, K.-F. Aoki-Kinoshita*, M. Fujita*, Global mapping of glycosylation pathways in human-derived cells. Developmental Cell, 56(8): 1195-1209 e1197 (2021)

28.S.-S Liu, Y.-S. Liu, X.-Y. Guo, Y. Murakami, G.-L. Yang, X.-D. Gao, T. Kinoshita, M. Fujita*, A knockout cell library of GPI biosynthetic genes for functional studies of GPI-anchored proteins, Communications Biology, 4(1): 777. doi.org/10.1038/s42003-021-02337-1 (2021)

29.J.-X. Leng, W.-W. Ren, Y.  Li, G.-L. Yang, X.-D. Gao, M Fujita*,      Cell engineering for the production of hybrid-type N-glycans in HEK293 cells, The Journal of Biochemistry, 170(1):139-151. doi: 10.1093/jb/mvab051 (2021)

30.F. Li , G.-L. Yang, H. Tachikawa, K. Shao, Y. Yang, X.-D. Gao, Nakanishi H*. Identification of novel O-GlcNAc transferase substrates using yeast cells expressing OGT, Journal of General and Applied Microbiology, 67(1): 33-41. (2021)

31.X.-M. Zhou, Y.-H. Zhai, C.-M. Liu, G.-L. Yang, J Guo, G Li, C.-W. Sun, X.-W. Qi, X Li, F Guan*. Sialidase NEU1 suppresses progression of human bladder cancer cells by inhibiting fibronectin-integrin α5β1 interaction and Akt signaling pathway, Cell Communication and Signaling 18(1): 1-15 (2020)

32.N Höti, T.-S. Lih, J.-B. Pan, Y.-Y. Zhou, G.-L. Yang, A. Deng, L.-J. Chen, M.-M. Dong, R.-B. Yang, C.-F.  Tu, M. C. Haffner, Q.-K. Li, H. Zhang*, A comprehensive analysis of FUT8 overexpressing prostate cancer cells reveals the role of EGFR in castration resistance, Cancers (Basel), 12(2): 468 doi: 10.3390/cancers12020468 (2020)

33.Y.-Y. Zhou, T.-S. Lih, G.-L. Yang, S.-Y. Chen, L.-J. Chen, D.-W. Chan, H. Zhang, Q.-K. Li*, An integrated workflow for global, glyco-, and phospho-proteomic analysis of tumor tissues, Analytical Chemistry, 92(2): 1842-1849 (2019)

34.S.-Y. Chen, M.-M. Dong, G.-L. Yang, Y.-Y. Zhou, D.J. Clark, T.-S. Lih, M. Schnaubelt, Z.-C. Liu, H. Zhang*, Glycans, glycosite, and intact glycopeptide analysis of N-Linked glycoproteins using liquid handling systems, Analytical Chemistry, 92 (2): 1680-1686 (2019)

35.Y.-Y. Zhou, T.-S. Lih, K. Cho, G.-L. Yang, Q.-K. Li*, Molecular analysis of bladder urothelial carcinoma using mass spectrometry-based, data-independent acquisition (DIA) approach in personalized medicine, Journal of the American Society of Cytopathology, 8 (5): S49-S50 (2019)

36.Q. Wang, G.-L. Yang, T Wang, W.-M. Yang, M.J. Betenbaugh, H. Zhang*, Characterization of intact glycopeptides reveals the impact of culture media on site-specific glycosylation of EPO-Fc fusion protein generated by CHO-GS cells, Biotechnology and Bioengineering, 116(9): 2303-2315 (2019)

37.Q Wang, C.-Y. Chung, W Yang, G.-L. Yang, S Chough, Y Chen, B.-J. Yin, R. Bhattacharya, Y.-W. Hu, C. T. Saeui, K. J. Yarema, M. J. Betenbaugh, H. Zhang*, Combining butyrated ManNAc with glycoengineered CHO cells improves EPO glycan quality and production, Biotechnology Journal, 14(4): e1800186. doi: 10.1002/biot.201800186 (2019)

38.X.-M. Zhou, S. Yang, G.-L. Yang, Z.-Q. Tan, F. Guan*, Two-step derivatization and mass spectral distinction of α2, 3 and α2, 6 sialic acid linkages on N-glycans by MALDI-TOF, Chinese Chemical Letters, 30 (3): 676-680 (2019)

39.Y.-N. Qin, Y Chen, G.-L. Yang, L. Zhao, Z. Shi*, Increased sialylation of N-and O-glycans on serum maackia amurensis lectin II binding glycoproteins in autistic spectrum disorder, Journal of Glycobiology, 8:137 (2019)

40.Y.-N. Qin, Y.-G. Zhong, T.-R. Ma, J.-X. Zhang, G.-L. Yang, F. Guan, Z. Li, B. Li*, A pilot study of salivary N-glycome in HBV-induced chronic hepatitis, cirrhosis, and hepatocellular carcinoma, Glycoconjugate Journal, 34 (4): 523-535 (2017)

41.T. Xiang, G.-L. Yang, X.Liu, Y. Zhou, Z. Fu, F. Lu, J. Gu, N. Taniguchi, Z.-Q. Tan, X. Chen., Y. Xie., F. Guan*, X.-L. Zhang*, Alteration of N-glycan  expression profile and glycan pattern of glycoproteins in human hepatoma cells after HCV infection, Biochimica et Biophysica Acta (BBA)-General Subjects, 1861(5): 1036-1045 (2017)

42.Z.-Q. Tan, Y. Zhou, G.-L. Yang, X Li, F Guan*, Lectin assisted glycoproteomics with bisecting GlcNAc structure. Journal of Agricultural Biotechnology, 25 (1), 74-83 (2017)

43.X. Li, F. Guan, D. Li, Z.-Q. Tan, G.-L. Yang, Y. Wu, Z.-H. Huang*, Identification of aberrantly expressed glycans in gastric cancer by integrated lectin microarray and mass spectrometric analyses, Oncotarget, 7 (52): 87284 (2016)

44.F. Guan, Z.-Q. Tan, X. Li, X.-C. Pang, Y. Zhu, D. Li, G.-L. Yang, A lectin-based isolation/enrichment strategy for improved coverage of N-glycan analysis, Carbohydrate Research, 416: 7-13 (2015)

45.X. Li, D. Li, X.-C. Pang, G.-L. Yang, H. J. Deeg, F. Guan*, Quantitative analysis of glycans, related genes, and proteins in two human bone marrow stromal cell lines using an integrated strategy. Experimental Hematology, 43(9): 760-769 e7 (2015)

46.J. Guo, Y.-C. Wang, B. Song, X. Wang, G.-L. Yang, F. Guan*, Identification and functional characterization of intracellular sialidase NeuA3 from Streptomyces avermitilis, Process Biochemistry, 50(5): 752-758 (2015)

47.J. Guo, B. Song, X. Li, G.-L. Yang, X. Yang, F. Guan*, Downregulation of gangliotetraosylceramide and β1,3-galactosyltransferase‑4 gene expression by Smads during transforming growth factor β‑induced epithelial‑mesenchymal transition, Molecular Medicine Reports, 11 (3): 2241-22477 (2015)

48.F. Guan, X. Li, J. Guo, G.-L. Yang, X. Li*, Ganglioside-magnetosome complex formation enhances uptake of gangliosides by cells, International Journal of Nanomedicine, 10: 6919 (2015)

49.Y.-N. Qin, Y.-G. Zhong, G.-L. Yang, T.-R. Ma, L. Jia, C. Huang, Z. Li*, Profiling of concanavalin A-binding glycoproteins in human hepatic stellate cells activated with transforming growth factor-β1, Molecules, 19(12):19845-19867 (2014)

50.J. Guo, X. Li, Z.-Q. Tan, W. Lu, G.-L. Yang, F. Guan*, Alteration of N-glycans and expression of their related glycogenes in the epithelial-mesenchymal transition of HCV29 bladder epithelial cells, Molecules, 19(12): 20073-20090 (2014)

51.Z.-Q. Tan, W Lu, X Li, G.-L. Yang, J Guo, H Yu, Z Li, F Guan*, Altered N-glycan expression profile in epithelial-to-mesenchymal transition of NMuMG cells revealed by an integrated strategy using mass spectrometry and glycogene and lectin microarray analysis, Journal of Proteome Research, 13(6): 2783-2795 (2014)

52.S.-S. Sun, G.-L. Yang, T. Wang, Q.-Z. Wang, C. Chen, Z. Li*, Isolation of N-linked glycopeptides by hydrazine-functionalized magnetic particles, Analytical and Bioanalytical Chemistry, 396(8):3071-3078 (2010)

53.X.-X. Sun, G.-L. Yang, S.-S. Sun, R. Quan, W. Dai, B. Li, C. Chen, Z. Li*, The hydroxyl-functionalized magnetic particles for purification of glycan-binding proteins, Current Pharmaceutical Biotechnology, 10(8):753-760 (2009)

54.G. Nan, H. Yan, G.-L. Yang, Q. Jian, C. Chen, Z. Li*, The hydroxyl-modified surfaces on glass support for fabrication of carbohydrate microarrays, Current Pharmaceutical Biotechnology 10(1):138-146 (2009)

专利成果:

1.一种快速分析生物样品中蛋白质及强极性长氨基酸序列糖肽的方法；杨刚龙、陈文彦;      ZL 2019 1 0764702.X.

2.一种牛奶外泌体的特征性标志物及鉴定牛奶外泌体的方法；杨刚龙、陈文彦、吴志猛、李丹；ZL 2019 1 0835803.1

3.一种牛奶外泌体的特征性糖蛋白标志物和牛奶外泌体的特征性标志物分离方法；杨刚龙、陈文彦、吴志猛；ZL 2020 1 1165566.1

4.一种亚细胞结构的特征性 N-连接糖链及其应用；杨刚龙、关锋、黄鲁瑜、郭佳；ZL 2016 1 0044194.4

5.一种两端标记相对定量分析糖链的方法；关锋、杨刚龙、谭增琦、陆微、庞星； 201410277160.0；

6.一种分离鉴定糖蛋白N-糖链结构的方法；关锋、杨刚龙、谭增琦、李想、郭佳、庞星辰；201410291021.3

7.一种细菌纳米磁小体-凝集素复合物及其制备方法与应用；关锋、李想、杨刚龙、郭佳、刘昌梅；CN201510075253.X

8.富集和纯化糖基化蛋白的方法；李铮、陈超、杨刚龙、崔亚丽；ZL200510096270.8

9.富集和纯化糖结合蛋白的方法； 李铮、陈超、杨刚龙、崔亚丽、惠文利； ZL200510096271.2

10.一种滤膜辅助分离生物样本中糖蛋白全Ｎ-连接糖链及其鉴别方法；李铮、杨刚龙、马恬然、王晔； 201210181029

11.一种滤膜辅助分离生物样本中糖蛋白全 O-连接糖链及其鉴别方法；李铮、杨刚龙、马恬然、王秦哲； 201210219469.5

12.一种分离纯化糖肽的方法；李铮、陈超、孙士生、杨刚龙、王婷、孙秀璇、邓玮娜； ZL200810017386.1