Recently, Professor Yin Dachuan’s research group has made progress in the study of protein self-assembly. The relevant research results were published in the excellent journal "Journal of Bioengineering" with an article entitled "Self-assembly phenomenon in transparent droplets formed by screening protein self-assembly conditions" .
This paper takes the interaction of β-lactoglobulin and protein crystallization kit IndexTM C10 as an example to explore. The experimental results show that there are micro-nano-scale protein self-assembly in the transparent droplet. The morphology of the micro-nano self-assembly in the transparent droplets formed by mixing different initial concentrations of β-lactoglobulin and IndexTM C10 was further observed by scanning electron microscope; the fluorescence-labeled β-lactograph was continuously photographed by the laser confocal microscope The process of globulin forming self-assembly, the liquid-liquid separation phenomenon and the morphology of the final self-assembly can be observed in real time; through in-situ X-ray diffraction, the internal structure of the self-assembly can be observed gradually An orderly process. The above research shows that in the protein self-assembly condition screening experiment based on the crystallization condition screening method, the self-assembly phenomenon in the transparent droplet is necessary and valuable for in-depth exploration.
Figure 1. Schematic diagram of the grafting process of fluorescent molecule f1 onto the surface of lactoglobulin
Figure 2. SEM results of lactoglobulin assembly formed by the interaction of β-lactoglobulin with IndexTM C10 at different concentrations
Dr. Zhang Tuodi from Northwestern Polytechnical University (the first unit) is the first author of the paper. Deng Xudong, Shi Wenpu, Chen Liangliang, Zhou Yaqing, Wang Xueting, Zhang Chenyan are the co-authors of this paper, and Professor Yin Dachuan is the corresponding author.
