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From trade‐off to synergy: microbial insights into enhancing plant growth and immunity Plant Biotech. J. (IF 13.8) Pub Date : 2024-05-12 Yee‐Shan Ku, Yi‐Jun Liao, Shian‐Peng Chiou, Hon‐Ming Lam, Ching Chan
SummaryThe reduction in crop yield caused by pathogens and pests presents a significant challenge to global food security. Genetic engineering, which aims to bolster plant defence mechanisms, emerges as a cost‐effective solution for disease control. However, this approach often incurs a growth penalty, known as the growth‐defence trade‐off. The precise molecular mechanisms governing this phenomenon
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Gland‐specific GhVQ22 negatively regulates gland size and affects secondary metabolic accumulation in cotton Plant Biotech. J. (IF 13.8) Pub Date : 2024-05-11 Peng‐Bao Wang, Xiang‐Fei Cheng, Peng‐Yu Wang, Xiao‐Lin Zhao, Lu Liu, Cheng‐De Yu, Roshan Zameer, Zhi‐Fang Li, Chun‐Peng Song, Chang‐Song Zou
Cotton (Gossypium spp.) has evolved pigment glands (PGs) that accumulate toxic terpenoids, such as gossypol, which serve as a defence mechanism against pests (Gadelha et al., 2014). Laboratory experiments and field trials have confirmed that PGs are essential for tolerance to chewing pests in cotton (Benedict et al., 1977; Mao et al., 2007). Altering the ability of PGs to synthesize and accumulate
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Protein complexes from edible mushrooms as a sustainable potato protection against coleopteran pests Plant Biotech. J. (IF 13.8) Pub Date : 2024-05-11 Karmen Pogačar, Maja Grundner, Primož Žigon, Anna Coll, Anastasija Panevska, Tjaša Lukan, Marko Petek, Jaka Razinger, Kristina Gruden, Kristina Sepčić
SummaryProtein complexes from edible oyster mushrooms (Pleurotus sp.) composed of pleurotolysin A2 (PlyA2) and pleurotolysin B (PlyB) exert toxicity in feeding tests against Colorado potato beetle (CPB) larvae, acting through the interaction with insect‐specific membrane sphingolipid. Here we present a new strategy for crop protection, based on in planta production of PlyA2/PlyB protein complexes,
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MicroRNA169 integrates multiple factors to modulate plant growth and abiotic stress responses Plant Biotech. J. (IF 13.8) Pub Date : 2024-05-08 Xiaotong Chen, Zhaohui Chen, Andrew Fiorentino, Morgan Kuess, Nishanth Tharayil, Rohit Kumar, Elizabeth Leonard, Rooksana Noorai, Qian Hu, Hong Luo
SummaryMicroRNA169 (miR169) has been implicated in multi‐stress regulation in annual species such as Arabidopsis, maize and rice. However, there is a lack of experimental functional and mechanistic studies of miR169 in plants, especially in perennial species, and its impact on plant growth and development remains unexplored. Creeping bentgrass (Agrostis stolonifera L.) is a C3 cool‐season perennial
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Multiplexed silencing of 2S albumin genes in peanut Plant Biotech. J. (IF 13.8) Pub Date : 2024-05-08 Joann A. Conner, Larissa Arrais Guimaraes, Zhifen Zhang, Kathleen Marasigan, Ye Chu, Walid Korani, Peggy Ozias‐Akins
Peanut (Arachis hypogaea L.) is a highly nutritious legume that provides energy-dense food. One obstacle for universal inclusion of peanut products in the diet is the prevalence of peanut food allergy. Although approximately 2% of individuals may become sensitized to peanut and experience an allergic reaction upon ingestion (Gupta et al., 2011), a minor fraction of these reactions may be life-threatening
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Expanding plant genome editing scope and profiles with CRISPR‐FrCas9 systems targeting palindromic TA sites Plant Biotech. J. (IF 13.8) Pub Date : 2024-05-07 Yao He, Yangshuo Han, Yanqin Ma, Shishi Liu, Tingting Fan, Yanling Liang, Xu Tang, Xuelian Zheng, Yuechao Wu, Tao Zhang, Yiping Qi, Yong Zhang
SummaryCRISPR‐Cas9 is widely used for genome editing, but its PAM sequence requirements limit its efficiency. In this study, we explore Faecalibaculum rodentium Cas9 (FrCas9) for plant genome editing, especially in rice. FrCas9 recognizes a concise 5′‐NNTA‐3′ PAM, targeting more abundant palindromic TA sites in plant genomes than the 5′‐NGG‐3′ PAM sites of the most popular SpCas9. FrCas9 shows cleavage
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Broad range plastid genome editing with monomeric TALE‐linked cytosine and dual base editors Plant Biotech. J. (IF 13.8) Pub Date : 2024-05-06 Xiaoyu Wang, Tyson Fang, Jason Lu, Leena Tripathi, Yiping Qi
Editing of the plastid genome helps understand the molecular functions of plastid genes and engineer desired traits in crops (Maliga, 2022). The DddA-derived cytosine base editors (DdCBEs) enable C-to-T editing in mitochondrial and plastid genomes (Kang et al., 2021; Li et al., 2021; Mok et al., 2020; Nakazato et al., 2021). Recently, Cho et al. (2022) developed TALE-linked deaminases (TALED) that
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UDP‐glycosyltransferase UGT96C10 functions as a novel detoxification factor for conjugating the activated dinitrotoluene sulfonate in switchgrass Plant Biotech. J. (IF 13.8) Pub Date : 2024-05-01 Kunlong Su, Zhenying Wu, Yuchen Liu, Yan Wang, Han Wang, Meifeng Liu, Yu Wang, Honglun Wang, Chunxiang Fu
SummaryDinitrotoluene sulfonates (DNTSes) are highly toxic hazards regulated by the Resource Conservation and Recovery Act (RCRA) in the United States. The trinitrotoluene (TNT) red water formed during the TNT purification process consists mainly of DNTSes. Certain plants, including switchgrass, reed and alfalfa, can detoxify low concentrations of DNTS in TNT red water‐contaminated soils. However,
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Greater mesophyll conductance and leaf photosynthesis in the field through modified cell wall porosity and thickness via AtCGR3 expression in tobacco Plant Biotech. J. (IF 13.8) Pub Date : 2024-04-30 Coralie E. Salesse‐Smith, Edward B. Lochocki, Lynn Doran, Benjamin E. Haas, Samantha S. Stutz, Stephen P. Long
SummaryMesophyll conductance (gm) describes the ease with which CO2 passes from the sub‐stomatal cavities of the leaf to the primary carboxylase of photosynthesis, Rubisco. Increasing gm is suggested as a means to engineer increases in photosynthesis by increasing [CO2] at Rubisco, inhibiting oxygenation and accelerating carboxylation. Here, tobacco was transgenically up‐regulated with Arabidopsis
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The Spartina alterniflora genome sequence provides insights into the salt‐tolerance mechanisms of exo‐recretohalophytes Plant Biotech. J. (IF 13.8) Pub Date : 2024-04-30 Shoukun Chen, Tingting Du, Zhangping Huang, Kunhui He, Maogeng Yang, Shang Gao, Tingxi Yu, Hao Zhang, Xiang Li, Shihua Chen, Chun‐Ming Liu, Huihui Li
SummarySpartina alterniflora is an exo‐recretohalophyte Poaceae species that is able to grow well in seashore, but the genomic basis underlying its adaptation to salt tolerance remains unknown. Here, we report a high‐quality, chromosome‐level genome assembly of S. alterniflora constructed through PacBio HiFi sequencing, combined with high‐throughput chromosome conformation capture (Hi‐C) technology
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Molecular farming for sustainable production of clinical‐grade antimicrobial peptides Plant Biotech. J. (IF 13.8) Pub Date : 2024-04-30 Shahid Chaudhary, Zahir Ali, Magdy Mahfouz
SummaryAntimicrobial peptides (AMPs) are emerging as next‐generation therapeutics due to their broad‐spectrum activity against drug‐resistant bacterial strains and their ability to eradicate biofilms, modulate immune responses, exert anti‐inflammatory effects and improve disease management. They are produced through solid‐phase peptide synthesis or in bacterial or yeast cells. Molecular farming, i
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The MdVQ37‐MdWRKY100 complex regulates salicylic acid content and MdRPM1 expression to modulate resistance to Glomerella leaf spot in apples Plant Biotech. J. (IF 13.8) Pub Date : 2024-04-29 Qinglong Dong, Dingyue Duan, Feng Wang, Kaiyu Yang, Yang Song, Yongxu Wang, Dajiang Wang, Zhirui Ji, Chengnan Xu, Peng Jia, Haoan Luan, Suping Guo, Guohui Qi, Ke Mao, Xuemei Zhang, Yi Tian, Yue Ma, Fengwang Ma
SummaryGlomerella leaf spot (GLS), caused by the fungus Colletotrichum fructicola, is considered one of the most destructive diseases affecting apples. The VQ‐WRKY complex plays a crucial role in the response of plants to biotic stresses. However, our understanding of the defensive role of the VQ‐WRKY complex on woody plants, particularly apples, under biotic stress, remains limited. In this study
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Engineering soybean with high levels of herbicide resistance with a Cas12-SF01-based cytosine base editor Plant Biotech. J. (IF 13.8) Pub Date : 2024-04-21 Qingfeng Niu, Hongtao Xie, Xuesong Cao, Minglei Song, Xin Wang, Shasha Li, Kang Pang, Yangyang Zhang, Jian-Kang Zhu, Jianhua Zhu
Soybean (Glycine max (L.) Merr.) is among the most important crops in the world for oil and proteins (Zhang et al., 2022). Given that weeds are a major challenge to soybean cultivation, non-transgenic herbicide-tolerant soybean varieties are in great demand, particularly in places where growing transgenic soybean is prohibited. The clustered regularly interspaced short palindromic repeats (CRISPR)-Cas
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OsALKBH9-mediated m6A demethylation regulates tapetal PCD and pollen exine accumulation in rice Plant Biotech. J. (IF 13.8) Pub Date : 2024-04-17 Jun Tang, Dekun Lei, Junbo Yang, Shuyan Chen, Xueping Wang, Xiaoxin Huang, Shasha Zhang, Zhihe Cai, Shanshan Zhu, Jianmin Wan, Guifang Jia
The N6-methyladenosine (m6A) mRNA modification is crucial for plant development and stress responses. In rice, the male sterility resulting from the deficiency of OsFIP37, a core component of m6A methyltransferase complex, emphasizes the significant role of m6A in male fertility. m6A is reversible and can be removed by m6A demethylases. However, whether mRNA m6A demethylase regulates male fertility
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Exploring the influence of a single‐nucleotide mutation in EIN4 on tomato fruit firmness diversity through fruit pericarp microstructure Plant Biotech. J. (IF 13.8) Pub Date : 2024-04-16 Shiwen Zhang, Shengqing Wu, Zhiqi Jia, Junhong Zhang, Ying Li, Xingyun Ma, Bingli Fan, Panqiao Wang, Yanna Gao, Zhibiao Ye, Wei Wang
SummaryTomato (Solanum lycopersicum) stands as one of the most valuable vegetable crops globally, and fruit firmness significantly impacts storage and transportation. To identify genes governing tomato firmness, we scrutinized the firmness of 266 accessions from core collections. Our study pinpointed an ethylene receptor gene, SlEIN4, located on chromosome 4 through a genome‐wide association study
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NF‐YC15 transcription factor activates ethylene biosynthesis and improves cassava disease resistance Plant Biotech. J. (IF 13.8) Pub Date : 2024-04-11 Liyan Zheng, Shuai Gao, Yujing Bai, Hongqiu Zeng, Haitao Shi
SummaryThe nuclear factor Y (NF‐Y) transcription factors play important roles in plant development and physiological responses. However, the relationship between NF‐Y, plant hormone and plant stress resistance in tropical crops remains unclear. In this study, we identified MeNF‐YC15 gene in the NF‐Y family that significantly responded to Xanthomonas axonopodis pv. manihotis (Xam) treatment. Using MeNF‐YC15‐silenced
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Soybean steroids improve crop abiotic stress tolerance and increase yield Plant Biotech. J. (IF 13.8) Pub Date : 2024-04-11 Tai‐Fei Yu, Ze‐Hao Hou, Hai‐Long Wang, Shi‐Yang Chang, Xin‐Yuan Song, Wei‐Jun Zheng, Lei Zheng, Ji‐Tong Wei, Zhi‐Wei Lu, Jun Chen, Yong‐Bin Zhou, Ming Chen, Su‐Li Sun, Qi‐Yan Jiang, Long‐Guo Jin, You‐Zhi Ma, Zhao‐Shi Xu
SummarySterols have long been associated with diverse fields, such as cancer treatment, drug development, and plant growth; however, their underlying mechanisms and functions remain enigmatic. Here, we unveil a critical role played by a GmNF‐YC9‐mediated CCAAT‐box transcription complex in modulating the steroid metabolism pathway within soybeans. Specifically, this complex directly activates squalene
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Detoxifying bacterial genes for deoxynivalenol epimerization confer durable resistance to Fusarium head blight in wheat Plant Biotech. J. (IF 13.8) Pub Date : 2024-04-09 Wei‐Jie He, Peng Yang, Tao Huang, Yu‐Fan Liu, Yu‐Wei Zhang, Wen‐Min Zhang, Tian‐Tian Zhang, Meng‐Ru Zheng, Ling Ma, Chang‐Xing Zhao, He‐Ping Li, Yu‐Cai Liao, Ai‐Bo Wu, Jing‐Bo Zhang
SummaryFusarium head blight (FHB) and the presence of mycotoxin deoxynivalenol (DON) pose serious threats to wheat production and food safety worldwide. DON, as a virulence factor, is crucial for the spread of FHB pathogens on plants. However, germplasm resources that are naturally resistant to DON and DON‐producing FHB pathogens are inadequate in plants. Here, detoxifying bacteria genes responsible
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The clade III subfamily of OsSWEETs directly suppresses rice immunity by interacting with OsHMGB1 and OsHsp20L Plant Biotech. J. (IF 13.8) Pub Date : 2024-04-08 Xin Wang, Yanhu Ju, Tao Wu, Lingguang Kong, Meng Yuan, Haifeng Liu, Xiangsong Chen, Zhaohui Chu
SummaryThe clade III subfamily of OsSWEETs includes transmembrane proteins necessary for susceptibility to bacterial blight (BB). These genes are targeted by the specific transcription activator‐like effector (TALE) of Xanthomonas oryzae pv. oryzae and mediate sucrose efflux for bacterial proliferation. However, the mechanism through which OsSWEETs regulate rice immunity has not been fully elucidated
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A viral protein competitively bound to rice CIPK23 inhibits potassium absorption and facilitates virus systemic infection in rice Plant Biotech. J. (IF 13.8) Pub Date : 2024-04-05 Xinxin Jing, Pengyue Wang, Jianjian Liu, Meirong Xiang, Xia Song, Chaonan Wang, Pengbai Li, Honglian Li, Zujian Wu, Chao Zhang
SummaryPotassium (K+) plays a crucial role as a macronutrient in the growth and development of plants. Studies have definitely determined the vital roles of K+ in response to pathogen invasion. Our previous investigations revealed that rice plants infected with rice grassy stunt virus (RGSV) displayed a reduction in K+ content, but the mechanism by which RGSV infection subverts K+ uptake remains unknown
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Site‐directed genotype screening for elimination of antinutritional saponins in quinoa seeds identifies TSARL1 as a master controller of saponin biosynthesis selectively in seeds Plant Biotech. J. (IF 13.8) Pub Date : 2024-04-04 Mai Duy Luu Trinh, Davide Visintainer, Jan Günther, Jeppe Thulin Østerberg, Rute R. da Fonseca, Sara Fondevilla, Max William Moog, Guangbin Luo, Anton F. Nørrevang, Christoph Crocoll, Philip V. Nielsen, Sven‐Erik Jacobsen, Toni Wendt, Søren Bak, Rosa Laura López‐Marqués, Michael Palmgren
SummaryClimate change may result in a drier climate and increased salinization, threatening agricultural productivity worldwide. Quinoa (Chenopodium quinoa) produces highly nutritious seeds and tolerates abiotic stresses such as drought and high salinity, making it a promising future food source. However, the presence of antinutritional saponins in their seeds is an undesirable trait. We mapped genes
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Development of marker‐free transgenic rice exhibiting stable and enhanced resistance to Rice ragged stunt virus and Rice grassy stunt virus via RNA interference Plant Biotech. J. (IF 13.8) Pub Date : 2024-04-04 Huiting Xie, Peng Gan, Shaoyuan Lü, Tianxin Tang, Chuan Li, Cheng Chen, Xin Guan, Zhihong Zhu, Yangdong Ye, Xianbo Huang, Shanshan Zhao, Jie Hu, Shuai Zhang, Jianguo Wu
Rice is the staple food for approximately half the global population, and rice virus diseases pose a serious threat to rice production, leading to significant losses in yield and quality, endangering agricultural security. Over the past two decades, significant advancements have been made by Chinese scientists in plant virology, encompassing basic research and the development of control measures for
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SlCRCa is a key D‐class gene controlling ovule fate determination in tomato Plant Biotech. J. (IF 13.8) Pub Date : 2024-04-02 Junqing Wu, Pengxue Li, Danyang Zhu, Haochuan Ma, Meng Li, Yixuan Lai, Yuxin Peng, Haixiao Li, Shuang Li, Jinbo Wei, Xinxin Bian, Abidur Rahman, Shuang Wu
SummaryCell fate determination and primordium initiation on the placental surface are two key events for ovule formation in seed plants, which directly affect ovule density and seed yield. Despite ovules form in the marginal meristematic tissues of the carpels, angiosperm carpels evolved after the ovules. It is not clear how the development of the ovules and carpels is coordinated in angiosperms. In
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Linkage and association mapping in multi-parental populations reveal the genetic basis of carotenoid variation in maize kernels Plant Biotech. J. (IF 13.8) Pub Date : 2024-03-28 Pengfei Yin, Xiuyi Fu, Haiying Feng, Yanyan Yang, Jing Xu, Xuan Zhang, Min Wang, Shenghui Ji, Binghao Zhao, Hui Fang, Xiaoxia Du, Yaru Li, Shuting Hu, Kun Li, Shutu Xu, Zhigang Li, Fang Liu, Yingni Xiao, Yuandong Wang, Jiansheng Li, Xiaohong Yang
Carotenoids are indispensable to plants and critical components of the human diet. The carotenoid metabolic pathway is conserved across plant species, but our understanding of the genetic basis of carotenoid variation remains limited for the seeds of most cereal crops. To address this issue, we systematically performed linkage and association mapping for eight carotenoid traits using six recombinant
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Vitamin B1 enhancement in the endosperm of rice through thiamine sequestration Plant Biotech. J. (IF 13.8) Pub Date : 2024-03-27 Teresa B. Fitzpatrick, Ivan Dalvit, Fei‐Han Chang, Kai Wang, Jared B. Fudge, Shu‐Heng Chang, Benoît Maillot, Wilhelm Gruissem
A core tenet of food security is ensuring people have access to sufficient nutritious food. Vitamin B1 (thiamine) is an essential micronutrient for humans, deficiency in which causes numerous diseases of the nervous and cardiovascular systems (Dhir et al., 2019). Such ailments are particularly associated with populations that have a high carbohydrate intake, and especially those that have sustenance
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Controlling diurnal flower‐opening time by manipulating the jasmonate pathway accelerates development of indica–japonica hybrid rice breeding Plant Biotech. J. (IF 13.8) Pub Date : 2024-03-25 Mumei Wang, Xiaopei Zhu, Zhen Huang, Minghao Chen, Peng Xu, Shitang Liao, Yongzhen Zhao, Yannan Gao, Jiahui He, Yutong Luo, Huixuan Chen, Xiaoying Wei, Shuai Nie, Jingfang Dong, Liya Zhu, Chuxiong Zhuang, Junliang Zhao, Zhenlan Liu, Hai Zhou
SummaryInter‐subspecific indica–japonica hybrid rice (Oryza sativa) has the potential for increased yields over traditional indica intra‐subspecies hybrid rice, but limited yield of F1 hybrid seed production (FHSP) hinders the development of indica–japonica hybrid rice breeding. Diurnal flower‐opening time (DFOT) divergence between indica and japonica rice has been a major contributing factor to this
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Development of a next generation SNP genotyping array for wheat Plant Biotech. J. (IF 13.8) Pub Date : 2024-03-23 Amanda J. Burridge, Mark Winfield, Alexandra Przewieslik‐Allen, Keith J. Edwards, Imteaz Siddique, Ruth Barral‐Arca, Simon Griffiths, Shifeng Cheng, Zejian Huang, Cong Feng, Susanne Dreisigacker, Alison R. Bentley, Gina Brown‐Guedira, Gary L. Barker
SummaryHigh‐throughput genotyping arrays have provided a cost‐effective, reliable and interoperable system for genotyping hexaploid wheat and its relatives. Existing, highly cited arrays including our 35K Wheat Breeder's array and the Illumina 90K array were designed based on a limited amount of varietal sequence diversity and with imperfect knowledge of SNP positions. Recent progress in wheat sequencing
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Bacterial growth‐mediated systems remodelling of Nicotiana benthamiana defines unique signatures of target protein production in molecular pharming Plant Biotech. J. (IF 13.8) Pub Date : 2024-03-22 Nicholas Prudhomme, Rebecca Pastora, Sarah Thomson, Edison Zheng, Amanda Sproule, Jonathan R. Krieger, J. Patrick Murphy, David P. Overy, Doug Cossar, Michael D. McLean, Jennifer Geddes‐McAlister
SummaryThe need for therapeutics to treat a plethora of medical conditions and diseases is on the rise and the demand for alternative approaches to mammalian‐based production systems is increasing. Plant‐based strategies provide a safe and effective alternative to produce biological drugs but have yet to enter mainstream manufacturing at a competitive level. Limitations associated with batch consistency
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Editing the nuclear localization signals of E1 and E1Lb enables the production of tropical soybean in temperate growing regions Plant Biotech. J. (IF 13.8) Pub Date : 2024-03-21 Yang Gao, Yuguo Zhang, Chuanyu Ma, Yanhui Chen, Chunxia Liu, Yanli Wang, Songyuan Wang, Xi Chen
SummarySoybean is a typical short‐day crop, and most commercial soybean cultivars are restricted to a relatively narrow range of latitudes due to photoperiod sensitivity. Photoperiod sensitivity hinders the utilization of soybean germplasms across geographical regions. When grown in temperate regions, tropical soybean responds to prolonged day length by increasing the vegetative growth phase and delaying
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Engineered poplar for bioproduction of the triterpene squalene Plant Biotech. J. (IF 13.8) Pub Date : 2024-03-20 Jacob D. Bibik, Abira Sahu, Boeun Kim, Faride Unda, Trine B. Andersen, Shawn D. Mansfield, Christos T. Maravelias, Thomas D. Sharkey, Björn R. Hamberger
SummaryBuilding sustainable platforms to produce biofuels and specialty chemicals has become an increasingly important strategy to supplement and replace fossil fuels and petrochemical‐derived products. Terpenoids are the most diverse class of natural products that have many commercial roles as specialty chemicals. Poplar is a fast growing, biomassdense bioenergy crop with many species known to produce
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OsKASI‐2 is required for the regulation of unsaturation levels of membrane lipids and chilling tolerance in rice Plant Biotech. J. (IF 13.8) Pub Date : 2024-03-20 Lin Zhang, Siyao Wang, Bin Bai, Yijun Chen, Zhipan Xiang, Chen Chen, Xuemei Kuang, Yuanzhu Yang, Jun Fu, Liangbi Chen, Dandan Mao
SummaryChilling stress has seriously limited the global production and geographical distribution of rice. However, the molecular mechanisms associated with plant responses to chilling stress are less known. In this study, we revealed a member of β‐ketoacyl‐ACP synthase I family (KASI), OsKASI‐2 which confers chilling tolerance in rice. OsKASI‐2 encodes a chloroplast‐localized KASI enzyme mainly expressed
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Expression of Concern: The efficacy of a novel insecticidal protein, Allium sativum leaf lectin (ASAL), against homopteran insects monitored in transgenic tobacco Plant Biotech. J. (IF 13.8) Pub Date : 2024-03-20
Dutta, I., Saha, P., Majumder, P., Sarkar, A., Chakraborti, D., Banerjee, S. and Das, S. (2005), The efficacy of a novel insecticidal protein, Allium sativum leaf lectin (ASAL), against homopteran insects monitored in transgenic tobacco. Plant Biotechnology Journal, 3: 601–611. https://doi.org/10.1111/j.1467-7652.2005.00151.x This Expression of Concern is for the above article, published online on
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Chromosome‐level reference genome and resequencing of 322 accessions reveal evolution, genomic imprint and key agronomic traits in adzuki bean Plant Biotech. J. (IF 13.8) Pub Date : 2024-03-18 Liwei Chu, Kai Yang, Chunhai Chen, Bo Zhao, Yanan Hou, Wanqiu Wang, Pu Zhao, Kaili Wang, Binhu Wang, Ying Xiao, Yongqiang Li, Yisong Li, Qijian Song, Biao Liu, Ruoxi Fan, Abhishek Bohra, Jianping Yu, Eva C. Sonnenschein, Rajeev K Varshney, Zhixi Tian, Jianbo Jian, Ping Wan
SummaryAdzuki bean (Vigna angularis) is an important legume crop cultivated in over 30 countries worldwide. We developed a high‐quality chromosome‐level reference genome of adzuki bean cultivar Jingnong6 by combining PacBio Sequel long‐read sequencing with short‐read and Hi‐C technologies. The assembled genome covers 97.8% of the adzuki bean genome with a contig N50 of approximately 16 Mb and a total
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An efficient genetic transformation system mediated by Rhizobium rhizogenes in fruit trees based on the transgenic hairy root to shoot conversion Plant Biotech. J. (IF 13.8) Pub Date : 2024-03-16 Lin Liu, Jinghua Qu, Chunyan Wang, Miao Liu, Chunmeng Zhang, Xinyue Zhang, Cheng Guo, Changai Wu, Guodong Yang, Jinguang Huang, Kang Yan, Huairui Shu, Chengchao Zheng, Shizhong Zhang
SummaryGenetic transformation is a critical tool for gene editing and genetic improvement of plants. Although many model plants and crops can be genetically manipulated, genetic transformation systems for fruit trees are either lacking or perform poorly. We used Rhizobium rhizogenes to transfer the target gene into the hairy roots of Malus domestica and Actinidia chinensis. Transgenic roots were generated
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PagARGOS promotes low‐lignin wood formation in poplar Plant Biotech. J. (IF 13.8) Pub Date : 2024-03-16 Xiaomin Yao, Guifang Zhang, Geng Zhang, Qian Sun, Cuimei Liu, Jinfang Chu, Yanping Jing, Shihui Niu, Chunxiang Fu, Tedrick Thomas Salim Lew, Jinxing Lin, Xiaojuan Li
SummaryWood formation, which occurs mainly through secondary xylem development, is important not only for supplying raw material for the ‘ligno‐chemical’ industry but also for driving the storage of carbon. However, the complex mechanisms underlying the promotion of xylem formation remain to be elucidated. Here, we found that overexpression of Auxin‐Regulated Gene involved in Organ Size (ARGOS) in
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A Verticillium dahliae exoglucanase as potential HIGS target interacts with a cotton cysteine protease to confer resistance to cotton Verticillium wilt Plant Biotech. J. (IF 13.8) Pub Date : 2024-03-16 Xiaofeng Su, Qi Wang, Tao Zhang, Xiaoyang Ge, Wende Liu, Huiming Guo, Xingfen Wang, Zhengwen Sun, Zhiqiang Li, Hongmei Cheng
Verticillium wilt, caused by the soil-borne pathogenic fungus Verticillium dahliae (Vd), represents a devastating disease impacting cotton (Gossypium spp.). However, the limited efficacy of measures to control Verticillium wilt arises because Vd colonizes the host vascular system, as well as the inherent resilience of Vd resting structures (microsclerotia), to various environmental influences (Fradin
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Development of a transgenic rice line with strong and broad resistance against four devastating rice viruses through expressing a single hairpin RNA construct Plant Biotech. J. (IF 13.8) Pub Date : 2024-03-16 Chenyang Li, Jianxiang Wu, Shuai Fu, Yi Xu, Yaqin Wang, Xiuling Yang, Ying Lan, Feng Lin, Linlin Du, Tong Zhou, Xueping Zhou
Rice is a staple food crop feeding nearly half of the world's population. However, rice production is threatened by devastating viral diseases such as rice black-streaked dwarf virus (RBSDV), southern rice black-streaked dwarf virus (SRBSDV), rice stripe virus (RSV) and rice ragged stunt virus (RRSV) (Wang et al., 2022a). Traditional breeding for viral resistance is challenging due to the lack of natural
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Recessive resistance against beet chlorosis virus is conferred by the eukaryotic translation initiation factor (iso)4E in Beta vulgaris Plant Biotech. J. (IF 13.8) Pub Date : 2024-03-16 Lukas Rollwage, Hilde Van Houtte, Roxana Hossain, Niels Wynant, Glenda Willems, Mark Varrelmann
SummaryEukaryotic translation initiation factors (eIFs) are important for mRNA translation but also pivotal for plant‐virus interaction. Most of these plant‐virus interactions were found between plant eIFs and the viral protein genome‐linked (VPg) of potyviruses. In case of lost interaction due to mutation or deletion of eIFs, the viral translation and subsequent replication within its host is negatively
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PTI‐ETI synergistic signal mechanisms in plant immunity Plant Biotech. J. (IF 13.8) Pub Date : 2024-03-12 Xiao‐Qian Yu, Hao‐Qiang Niu, Chao Liu, Hou‐Ling Wang, Weilun Yin, Xinli Xia
SummaryPlants face a relentless onslaught from a diverse array of pathogens in their natural environment, to which they have evolved a myriad of strategies that unfold across various temporal scales. Cell surface pattern recognition receptors (PRRs) detect conserved elicitors from pathogens or endogenous molecules released during pathogen invasion, initiating the first line of defence in plants, known
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Knockout of GRAIN WIDTH2 has a dual effect on enhancing leaf rust resistance and increasing grain weight in wheat Plant Biotech. J. (IF 13.8) Pub Date : 2024-03-09 Shujuan Liu, Hong Liu, Mengjiao Guo, Yuxue Pan, Chenyang Hao, Jian Hou, Liuling Yan, Xueyong Zhang, Xinhong Chen, Tian Li
Wheat leaf rust, caused by the fungal pathogen Puccinia triticina Eriksson (Pt), poses a major threat to global wheat production. The widespread presence and rapid evolution of Pt races lead to frequent epidemics, particularly in favourable climates, causing yield losses of 30%–50% (Prasad et al., 2020). Employing resistant genes and cultivars remains the most effective strategy against leaf rust.
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miR396b/GRF6 module contributes to salt tolerance in rice Plant Biotech. J. (IF 13.8) Pub Date : 2024-03-08 Huanran Yuan, Mingxing Cheng, Ruihua Wang, Zhikai Wang, Fengfeng Fan, Wei Wang, Fengfeng Si, Feng Gao, Shaoqing Li
SummarySalinity, as one of the most challenging environmental factors restraining crop growth and yield, poses a severe threat to global food security. To address the rising food demand, it is urgent to develop crop varieties with enhanced yield and greater salt tolerance by delving into genes associated with salt tolerance and high‐yield traits. MiR396b/GRF6 module has previously been demonstrated
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High‐throughput selective sweep SNP‐guided cloning of cold‐tolerance genes in rice Plant Biotech. J. (IF 13.8) Pub Date : 2024-03-07 Xiaoxia Li, Hongmei Wang, Chujian Xiao, Juan Huang, Yuting Long, Chuxin Lin, Yue Zhu, Man Wang, Yao‐Guang Liu, Qunyu Zhang
The genetic dissection and cloning of quantitative trait loci (QTLs) are prerequisites for implementing genomics-based applications in breeding programs. However, traditional positional cloning for QTLs is difficult, laborious and time-consuming. For example, more than 250 and 600 QTLs for cold tolerance have been detected in rice using genetic mapping and genome-wide association studies, respectively
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A higher‐yield hybrid rice is achieved by assimilating a dominant heterotic gene in inbred parental lines Plant Biotech. J. (IF 13.8) Pub Date : 2024-03-07 Changsheng Wang, Ziqun Wang, Yunxiao Cai, Zhou Zhu, Danheng Yu, Lei Hong, Yongchun Wang, Wei Lv, Qiang Zhao, Lizhen Si, Kun Liu, Bin Han
SummaryThe exploitation of heterosis to integrate parental advantages is one of the fastest and most efficient ways of rice breeding. The genomic architecture of heterosis suggests that the grain yield is strongly correlated with the accumulation of numerous rare superior alleles with positive dominance. However, the improvements in yield of hybrid rice have shown a slowdown or even plateaued due to
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CaIAA2‐CaARF9 module mediates the trade‐off between pepper growth and immunity Plant Biotech. J. (IF 13.8) Pub Date : 2024-03-07 Weiwei Cai, Yilin Tao, Xingge Cheng, Meiyun Wan, Jianghuang Gan, Sheng Yang, Thomas W. Okita, Shuilin He, Li Tian
SummaryTo challenge the invasion of various pathogens, plants re‐direct their resources from plant growth to an innate immune defence system. However, the underlying mechanism that coordinates the induction of the host immune response and the suppression of plant growth remains unclear. Here we demonstrate that an auxin response factor, CaARF9, has dual roles in enhancing the immune resistance to Ralstonia
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Comparisons of wild and cultivated American ginseng (Panax quinquefolius L.) genomes provide insights into changes in root growth and metabolism during domestication Plant Biotech. J. (IF 13.8) Pub Date : 2024-03-06 Zhengpeng Wang, Tengteng Wang, Jin Hu, Honghong Jiao, Yan Jin, Jiahui Sun, Tiegui Nan, Yuyang Zhao, Yunjun Liu, Luqi Huang, Yuan Yuan
American ginseng (Panax quinquefolius L.) originated in the forests of North America and was introduced to China over 40 years ago. Cultivated P. quinquefolius in China had fewer lateral roots and significantly decreased ginsenoside Rg1 content compared to wild American varieties, both of which determines quality and yield of P. quinquefolius (Figure S1). To explore genetic basis to alterations in
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A near complete genome of Arachis monticola, an allotetraploid wild peanut Plant Biotech. J. (IF 13.8) Pub Date : 2024-03-04 Hongzhang Xue, Kai Zhao, Kunkun Zhao, Suoyi Han, Annapurna Chitikineni, Lin Zhang, Ding Qiu, Rui Ren, Fangping Gong, Zhongfeng Li, Xingli Ma, Xingguo Zhang, Rajeev K. Varshney, Xinyou Zhang, Chaochun Wei, Dongmei Yin
Peanut (Arachis hypogaea L.) is the most economically important oilseed legume crops throughout the world. Most Arachis species are diploid, but two are heterotetraploid: one cultivated species (A. hypogaea) and one wild relative (A. monticola). A. monticola was an important intermediate between diploid wild peanut species and tetraploid cultivated peanut varieties (Zhuang et al., 2019). We released
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Control of two insect pests by expression of a mismatch corrected double‐stranded RNA in plants Plant Biotech. J. (IF 13.8) Pub Date : 2024-03-01 Yi Dong, Qi Zhang, Yarou Mao, Mengting Wu, Zican Wang, Ling Chang, Jiang Zhang
SummaryRNA interference (RNAi) has emerged as an efficient technology for pest control by silencing the essential genes of targeted insects. Owing to its nucleotide sequence‐guided working mechanism, RNAi has a high degree of species‐specificity without impacts on non‐target organisms. However, as plants are inevitably under threat by two or more insect pests in nature, the species‐specific mode of
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A method of genetic transformation and gene editing of succulents without tissue culture Plant Biotech. J. (IF 13.8) Pub Date : 2024-03-01 Jinghua Lu, Shanshan Li, Shuai Deng, Mugui Wang, Yinghuang Wu, Ming Li, Jinsong Dong, Suhui Lu, Chunli Su, Guofu Li, Zhaobo Lang, Jian‐Kang Zhu
SummarySucculents, valued for their drought tolerance and ornamental appeal, are important in the floriculture market. However, only a handful of succulent species can be genetically transformed, making it difficult to improve these plants through genetic engineering. In this study, we adapted the recently developed cut‐dip‐budding (CDB) gene delivery system to transform three previously recalcitrant
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Temperature and light reverse the fertility of rice P/TGMS line ostms19 via reactive oxygen species homeostasis Plant Biotech. J. (IF 13.8) Pub Date : 2024-02-29 Lei Zhou, Yi‐Chen Mao, Yan‐Ming Yang, Jun‐Jie Wang, Xiang Zhong, Yu Han, Yan‐Fei Zhang, Qiang‐Sheng Shi, Xue‐hui Huang, Blake C. Meyers, Jun Zhu, Zhong‐Nan Yang
SummaryP/TGMS (Photo/thermo‐sensitive genic male sterile) lines are crucial resources for two‐line hybrid rice breeding. Previous studies revealed that slow development is a general mechanism for sterility–fertility conversion of P/TGMS in Arabidopsis. However, the difference in P/TGMS genes between rice and Arabidopsis suggests the presence of a distinct P/TGMS mechanism in rice. In this study, we
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A 5.2‐kb insertion in the coding sequence of PavSCPL, a serine carboxypeptidase‐like enhances fruit firmness in Prunus avium Plant Biotech. J. (IF 13.8) Pub Date : 2024-02-28 Xiliang Qi, Yuanxin Dong, Congli Liu, Lulu Song, Lei Chen, Ming Li
SummaryFruit firmness is an important trait in sweet cherry breeding because it directly positively influences fruit transportability, storage and shelf life. However, the underlying genes responsible and the molecular mechanisms that control fruit firmness remain unknown. In this study, we identified a candidate gene, PavSCPL, encoding a serine carboxypeptidase‐like protein with natural allelic variation
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Genome editing of five starch synthesis genes produces highly resistant starch and dietary fibre in barley grains Plant Biotech. J. (IF 13.8) Pub Date : 2024-02-28 Qiang Yang, Jean‐Philippe Ral, Yuming Wei, Youliang Zheng, Zhongyi Li, Qiantao Jiang
Resistant starch (RS) refers to starch that is not digested in the stomach or small intestine, and provides health benefits by reducing glycaemic index and promoting gut health (Hazard et al., 2020). Barley (Hordeum vulgare L.) is the fourth most widely cultivated cereal worldwide, and there is growing interest in barley as a healthy food. The RS content of cereal grains is positively associated with
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Revisiting growth–defence trade-offs and breeding strategies in crops Plant Biotech. J. (IF 13.8) Pub Date : 2024-02-27 Mingjun Gao, Zeyun Hao, Yuese Ning, Zuhua He
Plants have evolved a multi-layered immune system to fight off pathogens. However, immune activation is costly and is often associated with growth and development penalty. In crops, yield is the main breeding target and is usually affected by high disease resistance. Therefore, proper balance between growth and defence is critical for achieving efficient crop improvement. This review highlights recent
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The TaSnRK1‐TabHLH489 module integrates brassinosteroid and sugar signalling to regulate the grain length in bread wheat Plant Biotech. J. (IF 13.8) Pub Date : 2024-02-27 Jinyang Lyu, Dongzhi Wang, Na Sun, Fan Yang, Xuepeng Li, Junyi Mu, Runxiang Zhou, Guolan Zheng, Xin Yang, Chenxuan Zhang, Chao Han, Guang‐Min Xia, Genying Li, Min Fan, Jun Xiao, Ming‐Yi Bai
SummaryRegulation of grain size is a crucial strategy for improving the crop yield and is also a fundamental aspect of developmental biology. However, the underlying molecular mechanisms governing grain development in wheat remain largely unknown. In this study, we identified a wheat atypical basic helix–loop–helix (bHLH) transcription factor, TabHLH489, which is tightly associated with grain length
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Mitochondrial genome editing of WA352 via mitoTALENs restore fertility in cytoplasmic male sterile rice Plant Biotech. J. (IF 13.8) Pub Date : 2024-02-27 Jiawei Zhou, Liyun Nie, Shuo Zhang, Hailiang Mao, Shin-ichi Arimura, Shuangxia Jin, Zhiqiang Wu
Cytoplasmic male sterility (CMS) is the inability of plants to produce functional pollen due to nucleo-cytoplasmic interaction (Wang et al., 2021). The CMS line is widely used in the three-line (CMS, maintainer and restorer) system to produce hybrid seeds with heterosis (Chen and Liu, 2014). Rice is one of the three major food crops in the world, and CMS-based hybrid rice technology has greatly improved
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Beyond pathways: Accelerated flavonoids candidate identification and novel exploration of enzymatic properties using combined mapping populations of wheat Plant Biotech. J. (IF 13.8) Pub Date : 2024-02-26 Jie Chen, Yueqi Zhang, Jiaqi Wei, Xin Hu, Huanran Yin, Wei Liu, Dongqin Li, Wenfei Tian, Yuanfeng Hao, Zhonghu He, Alisdair R. Fernie, Wei Chen
SummaryAlthough forward‐genetics‐metabolomics methods such as mGWAS and mQTL have proven effective in providing myriad loci affecting metabolite contents, they are somehow constrained by their respective constitutional flaws such as the hidden population structure for GWAS and insufficient recombinant rate for QTL. Here, the combination of mGWAS and mQTL was performed, conveying an improved statistical
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Optimizing ex vitro one‐step RUBY‐equipped hairy root transformation in drug‐ and hemp‐type Cannabis Plant Biotech. J. (IF 13.8) Pub Date : 2024-02-24 Ladan Ajdanian, Mohsen Niazian, Davoud Torkamaneh
Cannabis (Cannabis sativa L.), once concealed by the veil of prohibition, is now emerging as a versatile and promising plant species, riding the wave of recent legalization. This transformation has unlocked unprecedented opportunities for both medical research and industry growth, positioning cannabis on a trajectory to reach a projected market size of USD 444.34 Billion by 2030 (fortune business insights)
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ScRNA‐seq reveals dark‐ and light‐induced differentially expressed gene atlases of seedling leaves in Arachis hypogaea L. Plant Biotech. J. (IF 13.8) Pub Date : 2024-02-23 Quanqing Deng, Puxuan Du, Sunil S. Gangurde, Yanbin Hong, Yuan Xiao, Dongxiu Hu, Haifen Li, Qing Lu, Shaoxiong Li, Haiyan Liu, Runfeng Wang, Lu Huang, Wenyi Wang, Vanika Garg, Xuanqiang Liang, Rajeev K. Varshney, Xiaoping Chen, Hao Liu
SummaryAlthough the regulatory mechanisms of dark and light‐induced plant morphogenesis have been broadly investigated, the biological process in peanuts has not been systematically explored on single‐cell resolution. Herein, 10 cell clusters were characterized using scRNA‐seq‐identified marker genes, based on 13 409 and 11 296 single cells from 1‐week‐old peanut seedling leaves grown under dark and