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个人简介

教育与工作经历 1996年9月-2000年7月,河南农业大学,畜牧专业攻读本科 2006年9月-2012年7月,南京农业大学,硕博连读,动物遗传育种与繁殖专业攻读博士 2012年7月至今,在南京农业大学动物科技学院任教 奖励及荣誉 1.参加“基于“学研产”结合的动物繁殖学教学改革实践”,获2013年江苏省优秀教学成果二等奖; 2.参加“长三角区域肉羊规模化高效精准养殖关键技术集成与应用”,获农业部中华农业科技奖二等奖(6/15); 3.参加“依托肉羊产业,立足三全育人-卓越研究生培养体系探索与实践”,获南京农业大学研究生教学成果奖二等奖(3/6)。 参加学术团体及任职情况 中国畜牧兽医学会养羊学分会理事、动物繁殖学分会会员 承担科研项目 1. 国家自然科学基金面上项目:m6A甲基化修饰参与调控山羊体细胞核移植胚胎母源-合子转换期重编程的研究(主持,2020-2023); 2. 国家自然科学基金(青年):代谢谱与山羊克隆胚胎及其供体细胞发育潜能相关性研究(主持,2014-2016); 3. 中央高校基本科研业务费专项资金项目2项(主持,2014-2016、2018); 4. 江苏现代农业(肉羊)产业技术体系启东推广示范基地项目(主持,2019-); 5. 国家自然科学基金项目(面上):山羊早期卵泡和克隆胚胎发育中抗氧化机制的研究(参加,2013-2016); 6. 转基因生物新品种培育重大专项:高产优质转基因奶羊新品种培育(参加“乳铁蛋白转基因奶山羊新品种培育”任务,2008-2015) 7. 国家肉羊产业技术体系(参加,2016-2023); 8. 国家重点研发计划项目:山羊规模化高效安全养殖关键技术集成与示范(参加,2018-2020); 9. 国家科技支撑计划项目子任务:华南地区肉羊高效克隆胚胎生产与移植技术研究(参加,2011-2013); 10. 江苏省重点研发计划:优质肉用多胎绵羊新品系的选育及湖羊特色性状关键基因的挖掘(参加,2015-2018)。

研究领域

动物胚胎工程;肉羊繁育;羊业科学

动物遗传育种与繁殖;动物生物工程

近期论文

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论文论著: 围绕动物胚胎工程、肉羊的繁殖育种领域发表学术论文50余篇,其中第一或通讯作者发表SCI论文19篇,副主编参编教材、专著5部。 [1] Wan YJ*, Li DX, Deng MT, Liu ZF, Liu L, Wang F. Comprehensive Transcriptome Analysis of mRNA Expression Patterns of Early Embryo Development in Goat under Hypoxic and Normoxic Conditions[J]. Biology (Basel), 2021, 10(5): 381. [2] Deng MT#, Wan YJ#, Chen BB, Dai X, Liu ZF, Yang Y, Cai Y, Zhang Y*, Wang F*. Long non-coding RNA lnc_3712 impedes nuclear reprogramming via repressing Kdm5b[J]. Molecular Therapy - Nucleic Acids, 2021, 24: 54-66. [3] Liu ZF, Zhang GM, Deng MT, Yang H, Pang J, Cai Y, Wan YJ*, Wang F*. Inhibition of lysine-specific histone demethylase 1A results in meiotic aberration during oocyte maturation in vitro in goats[J]. Theriogenology, 2020, 143: 168-178. [4] Deng MT, Liu ZF, Chen BB, Cai Y, Wan YJ*, Wang F*. Locus-Specific Regulation of Xist Expression Using the CRISPR-Cas9-Based System[J]. DNA Cell Biol, 2020, 39(4): 572-578. [5] Deng MT, Zhang GM, Cai Y, Liu Z, Zhang Y, Meng F, Wang F, Wan YJ*. DNA methylation dynamics during zygotic genome activation in goat[J]. Theriogenology, 2020, 156: 144-154. [6] Wan YJ, Guo RH, Deng MT, Liu ZF, Pang J, Zhang GM, Wang ZY, Wang F*. Efficient generation of CLPG1-edited rabbits using the CRISPR/Cas9 system[J]. Reprod Domest Anim, 2019, 54(3): 538-544. [7] Ma JY, Ren CF, Yang H, Zhao J, Wang F*, Wan YJ*. The Expression Pattern of p32 in Sheep Muscle and Its Role in Differentiation, Cell Proliferation, and Apoptosis of Myoblasts[J]. Int J Mol Sci, 2019, 20(20): 5156. [8] Deng MT, Liu ZF, Ren CF, An SY, Wan YJ*, Wang F*. Highly methylated Xist in SCNT embryos was retained in deceased cloned female goats[J]. Reprod Fertil Dev, 2019, 31(5): 855–866. [9] Wan YJ, Deng MT, Zhang GM, Ren CF, Liu ZF, Wang F*. Analysis of H19/Igf2 Methylation Status in the Sperm of Cloned Goats and Their Offspring[J]. Cellular Reprogramming, 2018, 20(1): 66-75. [10] Deng MT, Liu ZF, Ren CF, Zhang GM, Pang J, Zhang Y, Wang F*, Wan YJ*. Long noncoding RNAs exchange during zygotic genome activation in goat[J]. Biol Reprod, 2018, 99(4): 707-717. [11] Deng MT, Ren CF, Liu ZF, Zhang GM, Wang F*, Wan YJ*. Epigenetic Status of H19-Igf2 Imprinted Genes and Loss of 5-Hydroxymethylcytosine in the Brain of Cloned Goats [J]. Cell Reprogram, 2017, 19(3): 199-207. [12] Zhou ZR, Jia RX, Zhang GM, Wan YJ*, Zhang YL, Fan YX, Wang ZY, Huang P, Wang F. Using cysteine/cystine to overcome oxidative stress in goat oocytes and embryos cultured in vitro [J]. Mol Med Rep, 2016, 14(2): 1219-1226. [13] Wan YJ, Deng MT, Zhang GM, Ren CF, Zhang H, Zhang YL, Wang LZ, Wang F. Abnormal expression of DNA methyltransferases and genomic imprinting in cloned goat fibroblasts [J]. Cell Biology International, 2016, 40(1): 74-82. [14] Guo RH#, Wan YJ#, Xu D, Cui L, Deng MT, Zhang GM, Jia RX, Zhou W, Wang Z, Deng K, Huang M, Wang F*, Zhang Y*. Generation and evaluation of Myostatin knock-out rabbits and goats using CRISPR/Cas9 system [J]. Sci Rep, 2016, 6: 29855. [15] 万永杰, 肖慎华, 邓明田, 张国敏, 贾若欣, 张艳丽, 王锋, 黄明睿*. 胚龄、季节和胚胎数量对转人乳铁蛋白基因克隆奶山羊生产效率的影响 [J]. 南京农业大学学报, 2015, 38(06): 980-985. [16] Zhang GM#, Wan YJ#, Zhang YL, Lan S, Jia RX, Wang ZY, Fan YX, Wang F*. Expression of Mitochondria-Associated Genes (PPARGC1A, NRF-1, BCL-2 and BAX) in Follicular Development and Atresia of Goat Ovaries [J]. Reprod Domest Anim, 2015, 50(3): 465-473. [17] Nie HT#, Wan YJ#, You JH, Wang ZY, Lan S, Fan YX, Wang F*. Effect of Age on Energy Requirement for Maintenance and Growth of Dorper and Hu Crossbred F1 Ewes Weighing 20 to 50 kg [J]. Asian-Australas J Anim Sci, 2015, 28(8): 1140-1149. [18] Meng L#, Wan YJ#, Sun YY, Zhang YL, Wang ZY, Song Y, Wang F. Generation of Five Human Lactoferrin Transgenic Cloned Goats Using Fibroblast Cells and Their Methylation Status of Putative Differential Methylation Regions of IGF2R and H19 Imprinted Genes [J]. PLoS One, 2013, 8(10): e77798. [19] Wan YJ, Zhang YL, Zhou ZR, Jia RX, Li M, Song H, Wang ZY, Wang LZ, Zhang GM, You JH, Wang F. Efficiency of donor cell preparation and recipient oocyte source for production of transgenic cloned dairy goats harboring human lactoferrin [J]. Theriogenology, 2012, 78(3): 583-592. 参编的著作 1. 参编《动物繁殖学实验教程》(第2版),中国农业大学出版社,2017 2. 副主编《肉羊标准化高效养殖关键技术》,江苏凤凰科学技术出版社,2016 3. 副主编《羊业科学研究进展(2015)》,中国农业科学技术出版社,2015 4. 参编《肉用山羊养殖与疫病防治新技术》,中国农业科学技术出版社,2015

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