Competition between RF1and suppressor tRNAs carrying noncanonical amino acids for recognising the amber codon is largely dependent on codon context. Here, we re‐explored this phenomenon by using blue–white screening of a library in which three randomised nucleotides were upstream and downstream of the amber codon, fused within the lacZ‐α gene.

中文翻译：

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通过蓝白筛选法重新探索大肠杆菌中非规范氨基酸的琥珀色密码子引导掺入的密码子上下文效应

RF1和携带非规范氨基酸的抑制性tRNA之间的竞争识别琥珀色密码子在很大程度上取决于密码子背景。在这里，我们通过对库进行蓝白筛选来重新研究这种现象，该库中三个随机核苷酸位于琥珀色密码子的上游和下游，并融合在lacZ- α基因中。