Black Phosphorus Hydrogel Scaffolds Enhance Bone Regeneration via a Sustained Supply of Calcium-Free Phosphorus,ACS Applied Materials & Interfaces

当前位置： X-MOL 学术 › ACS Appl. Mater. Interfaces › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Black Phosphorus Hydrogel Scaffolds Enhance Bone Regeneration via a Sustained Supply of Calcium-Free Phosphorus
ACS Applied Materials & Interfaces ( IF 8.3 ) Pub Date : 2018-12-31 00:00:00 , DOI: 10.1021/acsami.8b21179
Keqing Huang ₁ , Jun Wu ₁ , Zhipeng Gu ₁

Affiliation

Effective bone regeneration remains a challenge for bone-tissue engineering. In this study, we propose a new strategy to accelerate bone regeneration via a sustained supply of phosphorus without providing foreign calcium. Herein, a black phosphorus nanosheet (BPN)-based hydrogel platform was developed, and the BPNs were used to stably and mildly provide phosphorus. The hydrogel was fabricated by photo-crosslinking of gelatin methacrylamide, BPNs, and cationic arginine-based unsaturated poly(ester amide)s. This platform combines the following advantages: the hydrogel scaffold would keep BPNs inside, and the encapsulated BPNs can degrade into phosphorus ions and capture calcium ions to accelerate biomineralization in a bone defect. The introduction of BPNs helped to enhance the mechanical performance of hydrogels, photoresponsively release phosphate, and accelerate mineralization in vitro. Moreover, BPN-containing hydrogels improved osteogenic differentiation of human dental pulp stem cells via the bone morphogenic protein–runt-related transcription factor 2 pathway. In vivo results from a rabbit model of bone defects revealed that the BPNs helped to accelerate bone regeneration. All these results strongly suggest that the strategy of a sustained supply of calcium-free phosphorus and this BPN-containing hydrogel platform hold promise for effective bone regeneration.

中文翻译：

黑色磷水凝胶支架通过持续供应无钙磷来增强骨骼再生

有效的骨再生仍然是骨组织工程学的挑战。在这项研究中，我们提出了一种通过持续提供磷而不提供外来钙来加速骨骼再生的新策略。本文中，开发了一种基于黑磷纳米片（BPN）的水凝胶平台，并使用该BPN稳定，温和地提供了磷。通过明胶，甲基丙烯酰胺，BPNs和阳离子精氨酸基不饱和聚（酯酰胺）的光交联制备水凝胶。该平台具有以下优点：水凝胶支架可将BPN保留在内部，而封装的BPN则可降解为磷离子并捕获钙离子，从而加速骨骼缺损中的生物矿化作用。BPN的引入有助于增强水凝胶的机械性能，光响应性释放磷酸盐，并加速体外矿化。此外，含BPN的水凝胶可通过骨形态发生蛋白–矮种相关的转录因子2途径改善人类牙髓干细胞的成骨分化。兔骨骼缺陷模型的体内结果显示，BPNs有助于加速骨骼再生。所有这些结果都强烈表明，持续供应无钙磷和这种含BPN的水凝胶平台的策略有望实现有效的骨再生。兔骨骼缺陷模型的体内结果显示，BPNs有助于加速骨骼再生。所有这些结果都强烈表明，持续供应无钙磷和这种含BPN的水凝胶平台的策略有望实现有效的骨再生。兔骨骼缺陷模型的体内结果显示，BPNs有助于加速骨骼再生。所有这些结果都强烈表明，持续供应无钙磷和这种含BPN的水凝胶平台的策略有望实现有效的骨再生。

更新日期：2018-12-31

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南