Confinement of Singlet Oxygen Generated from Ruthenium Complex-Based Oxygen Sensor in the Pores of Mesoporous Silica Nanoparticles,Bioconjugate Chemistry

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Confinement of Singlet Oxygen Generated from Ruthenium Complex-Based Oxygen Sensor in the Pores of Mesoporous Silica Nanoparticles
Bioconjugate Chemistry ( IF 4.0 ) Pub Date : 2018-12-10 00:00:00 , DOI: 10.1021/acs.bioconjchem.8b00811
Natsuko Kitajima ₁ , Yui Umehara ₁ , Aoi Son ₁ , Teruyuki Kondo ₁ , Kazuhito Tanabe ₂

Affiliation

We synthesized mesoporous silica nanoparticles bearing ruthenium complexes in their pores (MSN-Ru) and characterized their photochemical properties. The ruthenium complexes that were immobilized in the pores showed oxygen-dependent phosphorescence, similar to the complexes that were not tethered to nanoparticles. Cellular imaging and in vivo experiments revealed that hypoxic cells and tissues could be visualized by monitoring the phosphorescence of MSN-Ru. Our most important finding was that the toxic effect of singlet oxygen (¹O₂), which was generated by excitation of the complexes, was effectively suppressed by the deactivation before leaking out from the pores. In addition, we observed a negligible toxic effect of the ruthenium complexes themselves due to the blockage of their direct interaction with intracellular biomolecules. Thus, MSN-Ru is a promising molecular probe of oxygen levels in living cells and tissues.

中文翻译：

基于钌配合物的氧传感器产生的单重态氧的限制在中孔二氧化硅纳米粒子的孔中

我们合成了在孔中带有钌配合物（MSN-Ru）的介孔二氧化硅纳米粒子，并表征了它们的光化学性质。固定在孔中的钌络合物显示出氧依赖性磷光，类似于未束缚在纳米颗粒上的络合物。细胞成像和体内实验表明，缺氧的细胞和组织可以通过监测MSN-Ru的磷光来可视化。我们最重要的发现是单重态氧（¹ O ₂由络合物的激发而产生的）被钝化有效抑制，然后从孔中泄漏出来。此外，我们观察到钌配合物本身与细胞内生物分子的直接相互作用受阻，其毒性作用可忽略不计。因此，MSN-Ru是活细胞和组织中氧水平的有前途的分子探针。

更新日期：2018-12-10

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