A new synthetic approach is established where both TiO₂ nanoparticles and glucose oxidase (GO_x) are together encapsulated into the cavity of ZIF-8 metal–organic framework (MOF) to fabricate a mediator-free glucose sensor in aqueous media. ZIF-8 possesses high stability both physically and chemically. Moreover, its large surface area and tunable cavity size are supportive to encapsulate both nanoparticles (TiO₂) and enzymes (GO_x). The as-synthesized nanocomposite is methodically characterized by various advanced analytical techniques, which suggests that TiO₂ is uniformly distributed within the cavity of ZIF-8 MOF. High surface area and double-layer capacitance of nanostructured TiO₂ jointly enhance the catalytic biosensor activity. The as-synthesized nanocomposite exhibits commendable stability and is able to detect low-level concentration (80 nM) of glucose in aqueous media by utilizing very low concentration of GO_x (62 μg in 1 mL).

中文翻译：

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MOF封装的TiO ₂平台在纳摩尔级的安培葡萄糖感测

建立了一种新的合成方法，将TiO ₂纳米颗粒和葡萄糖氧化酶（GO _x）一起封装到ZIF-8金属有机框架（MOF）的空腔中，以在水性介质中制造无介体的葡萄糖传感器。ZIF-8在物理和化学上都具有很高的稳定性。而且，其大的表面积和可调节的空腔尺寸有助于封装纳米颗粒（TiO ₂）和酶（GO _x）。合成后的纳米复合材料通过各种先进的分析技术进行了系统表征，这表明TiO ₂均匀分布在ZIF-8 MOF的腔体内。纳米结构TiO _2的高表面积和双层电容共同增强了催化生物传感器的活性。合成后的纳米复合材料表现出值得称赞的稳定性，并且能够通过利用极低浓度的GO _x（1 mL中62μg）来检测水性介质中的低浓度葡萄糖（80 nM ）。