Imbibition of Femtoliter-Scale DNA-Rich Aqueous Droplets into Porous Nylon Substrates by Molecular Printing.,Langmuir

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Imbibition of Femtoliter-Scale DNA-Rich Aqueous Droplets into Porous Nylon Substrates by Molecular Printing.
Langmuir ( IF 3.7 ) Pub Date : 2019-12-17 , DOI: 10.1021/acs.langmuir.9b02893
G Arrabito ₁ , V Ferrara ₂ , A Ottaviani ₃ , F Cavaleri ₁ , S Cubisino ₁ , P Cancemi ₄ , Y P Ho _{5,

6} , B R Knudsen _{7,

8} , M S Hede ₉ , C Pellerito ₁ , A Desideri ₃ , S Feo ₄ , B Pignataro ₁

Affiliation

This work presents the first reported imbibition mechanism of femtoliter (fL)-scale droplets produced by microchannel cantilever spotting (μCS) of DNA molecular inks into porous substrates (hydrophilic nylon). Differently from macroscopic or picoliter droplets, the downscaling to the fL-size leads to an imbibition process controlled by the subtle interplay of evaporation, spreading, viscosity, and capillarity, with gravitational forces being quasi-negligible. In particular, the minimization of droplet evaporation, surface tension, and viscosity allows for a reproducible droplet imbibition process. The dwell time on the nylon surface permits further tuning of the droplet lateral size, in accord with liquid ink diffusion mechanisms. The functionality of the printed DNA molecules is demonstrated at different imbibed oligonucleotide concentrations by hybridization with a fluorolabeled complementary sequence, resulting in a homogeneous coverage of DNA within the imbibed droplet. This study represents a first step toward the μCS-enabled fabrication of DNA-based biosensors and microarrays into porous substrates.

中文翻译：

Femtoliter规模的富含DNA的水滴通过分子印刷技术吸附到多孔尼龙基质中。

这项工作提出了第一个报告的飞升（fL）级液滴吸收机制，该液滴是通过将DNA分子墨水的微通道悬臂点样（μCS）注入多孔基质（亲水性尼龙）而产生的。与宏观或皮升液滴不同，缩小至fL尺寸会导致吸收过程，该过程受蒸发，扩散，粘度和毛细作用的微妙相互作用控制，而重力几乎可以忽略不计。特别地，液滴蒸发，表面张力和粘度的最小化允许可再现的液滴吸收过程。根据液体墨水扩散机制，在尼龙表面上的停留时间可进一步调整液滴的横向尺寸。通过与荧光标记的互补序列杂交，可以在不同的吸收寡核苷酸浓度下证明印出的DNA分子的功能，从而导致DNA在吸收液滴内的均匀覆盖。这项研究代表了将基于μCS的基于DNA的生物传感器和微阵列制造成多孔基质的第一步。

更新日期：2019-12-18

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