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A prevascularized nerve conduit based on a stem cell sheet effectively promotes the repair of transected spinal cord injury.
Acta Biomaterialia ( IF 9.4 ) Pub Date : 2019-10-31 , DOI: 10.1016/j.actbio.2019.10.042 Zengjie Fan 1 , Xiaozhu Liao 1 , Yu Tian 1 , Xie Xuzhuzi 1 , Yingying Nie 2
Acta Biomaterialia ( IF 9.4 ) Pub Date : 2019-10-31 , DOI: 10.1016/j.actbio.2019.10.042 Zengjie Fan 1 , Xiaozhu Liao 1 , Yu Tian 1 , Xie Xuzhuzi 1 , Yingying Nie 2
Affiliation
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Spinal cord injury (SCI) can result in severe loss of motor and sensory function caused by ischemia and hypoxia, which are the key limiting factors of SCI rehabilitation. Vascularization is considered an effective way to resolve the issues of ischemia and hypoxia. In this regard, we first fabricated prevascularized nerve conduits (PNC) based on the prevascularized stem cell sheet and evaluated their repair effects by implanting them into transected SCI rats. A better healing effect was presented in the PNC group than in the control group and the nonprevascularized nerve conduit (NPNC) group as shown in H&E staining and the Basso, Beattie, Bresnahan (BBB) Locomotor Rating Scale assessment. In addition, the expression of β-III tubulin (Tuj-1) in the PNC group was higher than that in the control group and the NPNC group because of the introduction of MSCs. Conversely, the expression of the glial fibrillary acidic protein (GFAP) in both experimental groups was lower than that in the control group because of the inhibitory effect of MSCs on glial scar formation. Taken together, the introduction of prevascularization into the neuron conduit was an effective solution for improving the condition of ischemia and hypoxia, inhibiting glial scar formation, and promoting the healing of SCI, which implied that the PNC may be a potential alternative material to biomaterials for SCI rehabilitation. STATEMENT OF SIGNIFICANCE: 1. Prevascularized stem cell sheet was first used to repair spinal cord injury (SCI). 2. Prevascularized stem cell sheet use can effectively resolve the challenges faced during SCI, including ischemia and hypoxia and the limited regenerative ability of the remained neurons. 3. Prevascularized stem cell sheet was found to accelerate the healing of SCI as compared to those in the control group and the pure stem cell sheet group. 4. The introduction of stem cells can effectively inhibit the formation of a glial scar.
中文翻译：
基于干细胞表皮的预血管化神经导管可有效促进横断性脊髓损伤的修复。
脊髓损伤（SCI）可能导致局部缺血和缺氧导致严重的运动和感觉功能丧失，这是SCI康复的关键限制因素。血管化被认为是解决缺血和缺氧问题的有效方法。在这方面，我们首先基于预先血管化的干细胞表制造了预先血管化的神经导管（PNC），并通过将它们植入横切SCI大鼠中来评估其修复效果。H＆E染色和Basso，Beattie，Bresnahan（BBB）运动自评量表评估显示，PNC组比对照组和非血管前神经导管（NPNC）组具有更好的愈合效果。此外，由于MSCs的引入，PNC组β-III微管蛋白（Tuj-1）的表达高于对照组和NPNC组。相反，由于MSCs对神经胶质瘢痕形成的抑制作用，两个实验组的神经胶质纤维酸性蛋白（GFAP）的表达均低于对照组。综上所述，将前血管形成引入神经元导管是改善缺血和缺氧状况，抑制神经胶质瘢痕形成并促进SCI愈合的有效解决方案，这意味着PNC可能是生物材料的潜在替代材料。 SCI康复。意义声明：1.首先将血管化的干细胞片用于修复脊髓损伤（SCI）。2。预血管化干细胞片的使用可以有效解决SCI期间面临的挑战，包括缺血和缺氧以及剩余神经元再生能力有限。3.与对照组和单纯干细胞片组相比，预血管化干细胞片可促进SCI的愈合。4.干细胞的引入可以有效抑制神经胶质瘢痕的形成。
更新日期：2019-11-01
中文翻译：
基于干细胞表皮的预血管化神经导管可有效促进横断性脊髓损伤的修复。
脊髓损伤（SCI）可能导致局部缺血和缺氧导致严重的运动和感觉功能丧失，这是SCI康复的关键限制因素。血管化被认为是解决缺血和缺氧问题的有效方法。在这方面，我们首先基于预先血管化的干细胞表制造了预先血管化的神经导管（PNC），并通过将它们植入横切SCI大鼠中来评估其修复效果。H＆E染色和Basso，Beattie，Bresnahan（BBB）运动自评量表评估显示，PNC组比对照组和非血管前神经导管（NPNC）组具有更好的愈合效果。此外，由于MSCs的引入，PNC组β-III微管蛋白（Tuj-1）的表达高于对照组和NPNC组。相反，由于MSCs对神经胶质瘢痕形成的抑制作用，两个实验组的神经胶质纤维酸性蛋白（GFAP）的表达均低于对照组。综上所述，将前血管形成引入神经元导管是改善缺血和缺氧状况，抑制神经胶质瘢痕形成并促进SCI愈合的有效解决方案，这意味着PNC可能是生物材料的潜在替代材料。 SCI康复。意义声明：1.首先将血管化的干细胞片用于修复脊髓损伤（SCI）。2。预血管化干细胞片的使用可以有效解决SCI期间面临的挑战，包括缺血和缺氧以及剩余神经元再生能力有限。3.与对照组和单纯干细胞片组相比，预血管化干细胞片可促进SCI的愈合。4.干细胞的引入可以有效抑制神经胶质瘢痕的形成。
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