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Structural Characterization and Chain Conformation of Water-Soluble β-Glucan from Wild Cordyceps sinensis
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2019-10-30 , DOI: 10.1021/acs.jafc.9b05340 Junqiao Wang 1 , Shuping Chen 1 , Shaoping Nie 1 , Steve W. Cui 1, 2 , Qi Wang 2 , Aled O. Phillips 3 , Glyn O. Phillips 4 , Mingyong Xie 1
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2019-10-30 , DOI: 10.1021/acs.jafc.9b05340 Junqiao Wang 1 , Shuping Chen 1 , Shaoping Nie 1 , Steve W. Cui 1, 2 , Qi Wang 2 , Aled O. Phillips 3 , Glyn O. Phillips 4 , Mingyong Xie 1
Affiliation
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Water-soluble β-d-glucan was obtained from wild Cordyceps sinensis by alkali solution and ethanol precipitation. The structure characteristics were determined using high-performance anion-exchange chromatography coupled with pulsed amperometric detection (HPAEC–PAD), methylation combined with gas chromatography–mass spectrometry, and one-/two-dimensional nuclear magnetic resonance spectroscopy. Results showed that β-d-glucan had a structure of every seven (1→3)-β-d-Glcp backbone residues with two (1→6)-β-d-Glcp branches. Additionally, conformation properties in different solvents were investigated by static light scattering, dynamic light scattering, and HPSEC with multiple detectors. It was found that β-d-glucan in 0.5 M NaOH had a narrow unimodal distribution of hydrodynamic radius displaying a spherical coil conformation, whereas it formed severe aggregation in dimethyl sulfoxide. In 0.1 M NaNO3, β-d-glucan mainly existed as a rod-like conformation corresponding to a helical structure together with small aggregates (10%). This work added more information to the understanding of C. sinensis polysaccharides.
中文翻译：
野生冬虫夏草水溶性β-葡聚糖的结构表征和链构象
通过碱溶液和乙醇沉淀从野生冬虫夏草中获得水溶性β- d-葡聚糖。使用高性能阴离子交换色谱法结合脉冲安培检测法（HPAEC–PAD），甲基化与气相色谱法–质谱联用以及一维/二维核磁共振波谱测定了结构特征。结果表明，β- d-葡聚糖具有每七个（1→3）-β- d- Glc p骨架残基的结构，具有两个（1→6）-β- d- Glc p骨架。分支机构。此外，还通过静态光散射，动态光散射和具有多个检测器的HPSEC研究了不同溶剂中的构象性质。发现在0.5M NaOH中的β- d-葡聚糖具有窄的流体力学半径的单峰分布，表现出球形线圈构象，而在二甲基亚砜中形成严重的聚集。在0.1M NaNO 3中，β- d-葡聚糖主要以棒状构型存在，对应于螺旋结构，且聚集体较小（10％）。这项工作为了解中华绒螯蟹多糖增添了更多信息。
更新日期：2019-11-01
中文翻译：
野生冬虫夏草水溶性β-葡聚糖的结构表征和链构象
通过碱溶液和乙醇沉淀从野生冬虫夏草中获得水溶性β- d-葡聚糖。使用高性能阴离子交换色谱法结合脉冲安培检测法（HPAEC–PAD），甲基化与气相色谱法–质谱联用以及一维/二维核磁共振波谱测定了结构特征。结果表明，β- d-葡聚糖具有每七个（1→3）-β- d- Glc p骨架残基的结构，具有两个（1→6）-β- d- Glc p骨架。分支机构。此外，还通过静态光散射，动态光散射和具有多个检测器的HPSEC研究了不同溶剂中的构象性质。发现在0.5M NaOH中的β- d-葡聚糖具有窄的流体力学半径的单峰分布，表现出球形线圈构象，而在二甲基亚砜中形成严重的聚集。在0.1M NaNO 3中，β- d-葡聚糖主要以棒状构型存在，对应于螺旋结构，且聚集体较小（10％）。这项工作为了解中华绒螯蟹多糖增添了更多信息。
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