单剂量暴露于雄性蜥蜴（Eremias argus）中的Prothioconazole和Prothioconazole-desthio后的性腺破坏。,Environmental Pollution

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单剂量暴露于雄性蜥蜴（Eremias argus）中的Prothioconazole和Prothioconazole-desthio后的性腺破坏。
Environmental Pollution ( IF 7.6 ) Pub Date : 2019-10-02 , DOI: 10.1016/j.envpol.2019.113297
Yun Xie ₁ , Haotian Jiang ₁ , Jing Chang ₂ , Yinghuan Wang ₂ , Jianzhong Li ₂ , Huili Wang ₂

Affiliation

Prothioconazole（PTC）是一种广泛使用的低毒性三唑类杀菌剂，据报道其脱硫代谢产物Prothioconazole-desthio（PTC-d）对哺乳动物具有更高的生殖毒性。但是，关于这两种化学物质对爬行动物的生殖毒性知之甚少，对内分泌的破坏作用则少得多。在这项研究中，我们调查了单剂量PTC / PTC-d（100 mg kg-1体重）暴露对睾丸和附睾的同态性，血清性类固醇激素（睾丸激素和17β-雌二醇）以及类固醇生成的转录的影响雄性蜥蜴（Eremias argus）的性腺中相关基因（STARD，cyp11A，cyp17，cyp19A，17β-HSD，3β-HSD，AR和ER-α）。尽管PTC-d暴露组中存在结构异常，但严重的性腺破坏，尤其是仅在PTC治疗后才在睾丸中观察到对精子发生的抑制作用，从而导致附睾管中缺乏精子。与该结果一致，与对照组相比，PTC暴露中的T / E2值升高到显着更高的水平，并随时间持续增加，而PTC-d暴露组中的T / E2值仅在12 h时才略有增加。这些结果表明，雄性蜥蜴性腺上PTC的破坏比PTC-d更严重。此外，cyp17基因的表达在6 h受到抑制，但在12 h被诱导，并且在每个时间点暴露于PTC后与STARD，cyp11A和3β-HSD呈负相关。PTC-d组的STARD和3β-HSD的表达明显下调，而cyp11A和cyp17的表达上调，并且每个基因随时间显示出一致的变化。对于17β-HSD，在两个治疗组中均未观察到显着性。这项研究是第一个比较雄性蜥蜴中PTC和PTC-d的性腺破坏的研究，并阐明了这两种化学物质通过差异转录调节影响了雄性腺的生理功能。

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Gonadal disruption after single dose exposure of prothioconazole and prothioconazole-desthio in male lizards (Eremias argus).

Prothioconazole (PTC) is a widely used triazole fungicide with low toxicity, and its desulfurization metabolite, prothioconazole-desthio (PTC-d), is reported to have higher reproductive toxicity to mammals. However, little is known about the reproductive toxicity, much less endocrine disrupting effect, of these two chemicals on reptiles. In this study, we investigated the effects of single dose of PTC/PTC-d (100 mg kg-1 body weight) exposure on the pathomorphism of testes and epididymides, serum sex steroid hormones (testosterone and 17β-estradiol) and transcription of steroidogenic-related genes (STARD, cyp11A, cyp17, cyp19A, 17β-HSD, 3β-HSD, AR and ER-α) in gonads of male lizards (Eremias argus). Although structural disorder existed in PTC-d exposure group, severe gonadal disruption, especially suppression of spermatogenesis was only observed in testis after PTC treatment, which consequently led to the lack of spermatozoa in epididymal ducts. Consistent with this result, T/E2 value in PTC exposure was elevated to a significant higher level compared with control and continually increased over time, while T/E2 value in the PTC-d exposure group slightly increased only at 12 h. These results demonstrated a more serious disruption of PTC on male lizard gonads than PTC-d. In addition, the expression of cyp17 gene was inhibited at 6 h, however, was induced at 12 h, and exhibited negative correlations with STARD, cyp11A and 3β-HSD after PTC exposure at each timepoint. In PTC-d group, the expression of STARD and 3β-HSD were significantly down-regulated, in contrast, cyp11A and cyp17 were up-regulated, and each gene showed consistent changes over time. For 17β-HSD, no significance was observed in both treated groups. This study was the first to compare the gonadal disruption of PTC and PTC-d in male lizards and elucidated that these two chemicals influenced the physiological function of male gonads through differential transcriptional modulation.

更新日期：2019-10-03

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