线性精子运动过程中产生的ROS对公猪精子线粒体基因表达和ATP产生的负面影响。,Free Radical Biology and Medicine

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线性精子运动过程中产生的ROS对公猪精子线粒体基因表达和ATP产生的负面影响。
Free Radical Biology and Medicine ( IF 7.1 ) Pub Date : 2019-06-15 , DOI: 10.1016/j.freeradbiomed.2019.06.018
Zhendong Zhu ₁ , Tomoko Kawai ₂ , Takashi Umehara ₂ , S A Masudul Hoque ₃ , Wenxian Zeng ₄ , Masayuki Shimada ₂

Affiliation

线粒体氧化磷酸化（OXPHOS）对于从子宫迁移到输卵管期间ATP维持精子线性运动至关重要。但是，ROS是OXPHOS的副产品，会产生压力并损害精子质量。这项研究旨在阐明精子线粒体中能降低线性运动性的ROS靶标，并研究线粒体靶标抗氧化剂（PQQ和CoQ10）是否影响线粒体活性和精子运动性。在低葡萄糖培养基中温育期间，随着ROS水平的提高，精子的线性运动模式，ATP产生和线粒体活性降低。然而，在高糖培养基中，精子活动模式和ROS水平没有明显改变。此外，基因表达系统（mt-DNA，在低葡萄糖培养基中温育期间，精子线粒体中的线粒体转录因子-A（TFAM）和RNA聚合酶（POLRMT）受到破坏。有趣的是，PQQ处理提高了mt-DNA的稳定性，并减少了对TFAM和POLRMT的破坏，从而导致线粒体基因的高表达。此外，抗氧化剂在低葡萄糖条件下增加了线粒体的活性并保持了精子的线性运动。这些结果表明，随着精子中ROS含量的增加，ATP的产生和线粒体转录系统都受到损害，而精子中的ROS含量呈线性变化。用抗氧化剂（例如PQQ和CoQ10）进行治疗是保持精子线性运动的有益工具。PQQ处理提高了mt-DNA的稳定性，并减少了对TFAM和POLRMT的破坏，从而导致线粒体基因的高表达。此外，抗氧化剂在低葡萄糖条件下增加了线粒体的活性并保持了精子的线性运动。这些结果表明，随着精子中ROS含量的增加，ATP的产生和线粒体转录系统都受到损害，而精子中的ROS含量呈线性变化。用抗氧化剂（例如PQQ和CoQ10）进行治疗是保持精子线性运动的有益工具。PQQ处理提高了mt-DNA的稳定性，并减少了对TFAM和POLRMT的破坏，从而导致线粒体基因的高表达。此外，抗氧化剂在低葡萄糖条件下增加了线粒体的活性并保持了精子的线性运动。这些结果表明，随着精子中ROS含量的增加，ATP的产生和线粒体转录系统都受到损害，而精子中的ROS含量呈线性变化。用抗氧化剂（例如PQQ和CoQ10）进行治疗是保持精子线性运动的有益工具。这些结果表明，随着精子中ROS含量的增加，ATP的产生和线粒体转录系统都受到损害，而精子中的ROS含量呈线性变化。用抗氧化剂（例如PQQ和CoQ10）进行治疗是保持精子线性运动的有益工具。这些结果表明，随着精子中ROS含量的增加，ATP的产生和线粒体转录系统都受到损害，而精子中的ROS含量呈线性变化。用抗氧化剂（例如PQQ和CoQ10）进行治疗是保持精子线性运动的有益工具。

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Negative effects of ROS generated during linear sperm motility on gene expression and ATP generation in boar sperm mitochondria.

Mitochondrial oxidative phosphorylation (OXPHOS) is essential for ATP production to maintain sperm linear motility during migration from the uterus to the oviduct. However, ROS are generated as by-products of OXPHOS, causing stress and damaging the sperm quality. This study aimed to clarify the ROS targets in sperm mitochondria that decrease linear motility and to investigate whether mitochondria-target antioxidants (PQQ and CoQ10) affect mitochondrial activity and sperm motility. Sperm linear motility pattern, ATP production, and mitochondrial activity were decreased with increasing ROS levels during incubation in the low-glucose medium. However, sperm motility patterns and ROS levels were not significantly changed in the high-glucose medium. Moreover, the gene expression system (mt-DNA, mitochondrial transcription factor-A (TFAM) and RNA polymerase (POLRMT)) in sperm mitochondria was damaged during incubation in the low-glucose medium. Interestingly, PQQ treatment increased the mt-DNA stability and decreased the damage to TFAM and POLRMT, which resulted in high expression of mitochondrial genes. Furthermore, the antioxidants increased mitochondrial activity and maintained sperm linear motility under the low glucose condition. These results revealed that both ATP production and the mitochondrial transcription system are damaged with increasing ROS levels in sperm that show a linear motility pattern. Treatment with antioxidants, such as PQQ and CoQ10, is beneficial tool to maintain sperm linear motility.

更新日期：2019-06-16

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