Fenton Reaction-Oxidized Bamboo Lignin Surface and Structural Modification to Reduce Nonproductive Cellulase Binding and Improve Enzyme Digestion of Cellulose,ACS Sustainable Chemistry & Engineering

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Fenton Reaction-Oxidized Bamboo Lignin Surface and Structural Modification to Reduce Nonproductive Cellulase Binding and Improve Enzyme Digestion of Cellulose
ACS Sustainable Chemistry & Engineering ( IF 7.1 ) Pub Date : 2018-01-23 00:00:00 , DOI: 10.1021/acssuschemeng.7b04191
Kai Wu ₁ , Wenjun Ying ₁ , Zhengjun Shi _{1,

2} , Haiyan Yang _{1,

2} , Zhifeng Zheng ₂ , Jiayan Zhang ₁ , Jing Yang _{1,

2}

Affiliation

Nonproductive adsorption of cellulase on lignin was a major inhibitory mechanism reducing the enzymatic saccharification efficiency of lignocellulosic material. The surface and structural properties of Fenton reaction-oxidized lignins (FRLs) and their effects on the nonproductive binding of enzymes to lignin and enzymatic saccharification of Avicel were examined. The Fenton chemistry pretreatment not only decreased the ratio of syringyl to guaiacyl units and the phenolic OH group content in lignin preparations but increased the carboxylic content and the negative zeta potential of lignin, all of which could be responsible for reducing the unproductive adsorption of cellulase on lignin. And Langmuir adsorption isotherms also proved that FRLs were provided with a lower binding ability to the cellulase enzyme when compared with untreated milled bamboo lignin (MBL). Finally, when the nonproductive binding of cellulase to FRLs was reduced significantly, the 72 h glucose yields of Avicel with 3-FRL and 5-FRL were 60.64% and 65.96%, respectively, which were 6.3–15.6% higher than that of Avicel alone, 57.06%. The results can also help to optimize the existing pretreatment technologies and develop new pretreatment methods that could produce less inhibitory lignin.

中文翻译：

Fenton反应氧化的竹木质素表面和结构修饰，以减少非生产性纤维素酶的结合并改善纤维素的酶消化

纤维素酶在木质素上的非生产性吸附是降低木质纤维素材料的酶促糖化效率的主要抑制机制。研究了Fenton反应氧化木质素（FRLs）的表面和结构特性，以及它们对酶与木质素的非生产性结合以及Avicel的酶促糖化作用的影响。Fenton化学预处理不仅降低了木质素制剂中丁香基对愈创木基单元的比率和酚式OH基含量，而且增加了木质素的羧基含量和负ζ电势，所有这些都可以减少纤维素酶在纤维素上的非生产性吸附。木质素。Langmuir吸附等温线还证明，与未处理的碾磨竹木质素（MBL）相比，FRL与纤维素酶的结合能力较低。最后，当纤维素酶与FRL的非生产性结合显着降低时，具有3-FRL和5-FRL的Avicel的72 h葡萄糖产率分别为60.64％和65.96％，比单独的Avicel高6.3–15.6％。，为57.06％。结果还可以帮助优化现有的预处理技术，并开发出可以产生较少抑制性木质素的新预处理方法。

更新日期：2018-01-23

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