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SiR-Hoechst is a far-red DNA stain for live-cell nanoscopy.
Nature Communications ( IF 14.7 ) Pub Date : 2015-Oct-01 , DOI: 10.1038/ncomms9497
Gražvydas Lukinavičius 1 , Claudia Blaukopf 2 , Elias Pershagen 3 , Alberto Schena 1 , Luc Reymond 1 , Emmanuel Derivery 4 , Marcos Gonzalez-Gaitan 4 , Elisa D'Este 5 , Stefan W Hell 5 , Daniel Wolfram Gerlich 2 , Kai Johnsson 1
Affiliation  

Cell-permeable DNA stains are popular markers in live-cell imaging. Currently used DNA stains for live-cell imaging are either toxic, require illumination with blue light or are not compatible with super-resolution microscopy, thereby limiting their utility. Here we describe a far-red DNA stain, SiR-Hoechst, which displays minimal toxicity, is applicable in different cell types and tissues, and is compatible with super-resolution microscopy. The combination of these properties makes this probe a powerful tool for live-cell imaging.

中文翻译:

SiR-Hoechst 是一种用于活细胞纳米镜检查的远红 DNA 染料。

可渗透细胞的 DNA 染色剂是活细胞成像中常用的标记物。目前用于活细胞成像的 DNA 染色剂要么有毒,要么需要蓝光照射,要么与超分辨率显微镜不兼容,从而限制了它们的实用性。在这里,我们描述了一种远红色 DNA 染色剂 SiR-Hoechst,它显示出最小的毒性,适用于不同的细胞类型和组织,并且与超分辨率显微镜兼容。这些特性的结合使该探针成为活细胞成像的强大工具。
更新日期:2015-10-04
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