<span>S层蛋白作为类胡萝卜素的来源：从其S层蛋白DR_2577中分离类胡萝卜素辅因子去黄嘌呤</span>,Food Research International

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S层蛋白作为类胡萝卜素的来源：从其S层蛋白DR_2577中分离类胡萝卜素辅因子去黄嘌呤
Food Research International ( IF 7.0 ) Pub Date : 2016-10-11 , DOI: 10.1016/j.foodres.2016.10.003
Domenica Farci , Francesca Esposito , Sabah El Alaoui , Dario Piano

S层是蛋白质或糖蛋白的规则副晶体排列，是几种细菌和古细菌的外被膜的特征。这些蛋白质的自组装特性使其适用于纳米技术。但是，细菌细胞壁及其S层也是类胡萝卜素的重要结合位点，它们可能代表了这些用于工业目的的珍贵分子的潜在来源。在耐辐射细菌Deinococcus radiodurans中广泛研究了S层结构及其成分。，长期以来就代表了这方面的典范生物之一。已经证明蛋白质DR_2577是该细菌中自然过度表达的S层成分之一。本报告描述了该蛋白在溶液中的大规模纯化程序。通过自然电泳和变性电泳分析的样品纯度显示了该方法如何导致对纯DR_2577的选择性和高效回收。最近，我们发现，deinoxanthin，类胡萝卜素典型的耐辐射奇球菌是与蛋白质DR_2577非共价结合的辅因子。可以将纯的DR_2577样品沉淀或冻干，并通过使用有机溶剂进行有效萃取来用作类胡萝卜素辅因子去黄嘌呤的来源。这项工作中描述的程序可能代表了一种分离S层蛋白及其类胡萝卜素的通用方法，具有工业应用的潜力。

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S-layer proteins as a source of carotenoids: Isolation of the carotenoid cofactor deinoxanthin from its S-layer protein DR_2577

S-layers are regular paracrystalline arrays of proteins or glycoproteins that characterize the outer envelope of several bacteria and archaea. The auto-assembling properties of these proteins make them suitable for application in nanotechnologies. However, the bacterial cell wall and its S-layer are also an important binding sites for carotenoids and they may represent a potential source of these precious molecules for industrial purposes. The S-layer structure and its components were extensively studied in the radio-resistant bacterium Deinococcus radiodurans, which for long time represented one of the model organisms in this respect. The protein DR_2577 has been shown to be one of the naturally over-expressed S-layer components in this bacterium. The present report describes a high scale purification procedure of this protein in solution. The purity of the samples, assayed by native and denaturing electrophoresis, showed how this method leads to a selective and high efficient recovery of the pure DR_2577. Recently, we have found that the deinoxanthin, a carotenoid typical of D. radiodurans, is a cofactor non covalently bound to the protein DR_2577. The pure DR_2577 samples may be precipitated or lyophilized and used as a source of the carotenoid cofactor deinoxanthin by an efficient extraction using organic solvents. The procedure described in this work may represent a general approach for the isolation of S-layer proteins and their carotenoids with potentials for industrial applications.

更新日期：2017-08-30

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