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Gold Nanoparticle Loaded Split-DNAzyme Probe for Amplified miRNA Detection in Living Cells
Analytical Chemistry ( IF 6.7 ) Pub Date : 2017-07-28 00:00:00 , DOI: 10.1021/acs.analchem.7b01632 Yanan Wu 1 , Jin Huang 1 , Xiaohai Yang 1 , Yanjing Yang 1 , Ke Quan 1 , Nuli Xie 1 , Jing Li 1 , Changbei Ma 2 , Kemin Wang 1
Analytical Chemistry ( IF 6.7 ) Pub Date : 2017-07-28 00:00:00 , DOI: 10.1021/acs.analchem.7b01632 Yanan Wu 1 , Jin Huang 1 , Xiaohai Yang 1 , Yanjing Yang 1 , Ke Quan 1 , Nuli Xie 1 , Jing Li 1 , Changbei Ma 2 , Kemin Wang 1
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A new class of intracellular nanoprobe, termed AuNP loaded split-DNAzyme probe, was developed to sense miRNA in living cells. Briefly, it consists of an AuNP and substrates hybridized with two half of split DNAzymes. In the absence of target miRNA, the split DNAzymes form an inactive DNAzyme motif with their substrate through partial paring at the end of each strand, and the fluorescence is quenched. Inside the cells, the target miRNA binds with both of the two half of split DNAzymes, forming the active secondary structure in the catalytic cores, which can cleave the substrates, resulting in the rupture of the substrate and recovery of the fluorescence. Meanwhile, the target is released and binds to another inactive DNAzyme motif to drive another cycle of activation. During the cyclic process, a very small number of target miRNAs can initiate the cleavage of many fluorophore-labeled substrate strands from AuNP surface, providing an amplified fluorescent signal of the target miRNA and, thus, offering high detection sensitivity.
中文翻译：
金纳米粒子加载分裂DNAzyme探针，用于在活细胞中扩增miRNA检测。
开发了一种新型的细胞内纳米探针，称为AuNP加载的split-DNAzyme探针，用于检测活细胞中的miRNA。简而言之，它由AuNP和与分裂的两半DNAzyme杂交的底物组成。在不存在靶标miRNA的情况下，分裂的DNA酶通过在每条链的末端部分配对，与其底物形成无活性的DNA酶基序，并且荧光被淬灭。在细胞内部，靶标miRNA与分裂的DNAzyme的两半结合，在催化核心中形成活性二级结构，可以裂解底物，导致底物破裂并恢复荧光。同时，靶标被释放并结合到另一个非活性DNAzyme基序，以驱动另一个激活周期。在循环过程中，
更新日期：2017-07-30
中文翻译：
金纳米粒子加载分裂DNAzyme探针，用于在活细胞中扩增miRNA检测。
开发了一种新型的细胞内纳米探针，称为AuNP加载的split-DNAzyme探针，用于检测活细胞中的miRNA。简而言之，它由AuNP和与分裂的两半DNAzyme杂交的底物组成。在不存在靶标miRNA的情况下，分裂的DNA酶通过在每条链的末端部分配对，与其底物形成无活性的DNA酶基序，并且荧光被淬灭。在细胞内部，靶标miRNA与分裂的DNAzyme的两半结合，在催化核心中形成活性二级结构，可以裂解底物，导致底物破裂并恢复荧光。同时，靶标被释放并结合到另一个非活性DNAzyme基序，以驱动另一个激活周期。在循环过程中，
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