Field-Free Isolation of Exosomes from Extracellular Vesicles by Microfluidic Viscoelastic Flows,ACS Nano

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Field-Free Isolation of Exosomes from Extracellular Vesicles by Microfluidic Viscoelastic Flows
ACS Nano ( IF 15.8 ) Pub Date : 2017-07-07 00:00:00 , DOI: 10.1021/acsnano.7b02277
Chao Liu ₁ , Jiayi Guo _{1,

2} , Fei Tian ₁ , Na Yang ₁ , Fusheng Yan ₁ , Yanping Ding ₁ , JingYan Wei ₂ , Guoqing Hu _{3,

4} , Guangjun Nie _{1,

4} , Jiashu Sun _{1,

4}

Affiliation

Exosomes, molecular cargos secreted by almost all mammalian cells, are considered as promising biomarkers to identify many diseases including cancers. However, the small size of exosomes (30–200 nm) poses serious challenges in their isolation from complex media containing a variety of extracellular vesicles (EVs) of different sizes, especially in small sample volumes. Here we present a viscoelasticity-based microfluidic system to directly separate exosomes from cell culture media or serum in a continuous, size-dependent, and label-free manner. Using a small amount of biocompatible polymer as the additive in the media to control the viscoelastic forces exerted on EVs, we are able to achieve a high separation purity (>90%) and recovery (>80%) of exosomes. The proposed technique may serve as a versatile platform to facilitate exosome analyses in diverse biochemical applications.

中文翻译：

微流粘弹性流从细胞外囊的外泌体的无场分离。

外泌体是几乎所有哺乳动物细胞分泌的分子货物，被认为是鉴别包括癌症在内的许多疾病的有前途的生物标志物。然而，小体积的外泌体（30-200 nm）在将它们与包含各种不同大小的各种细胞外囊泡（EV）的复杂培养基分离时提出了严峻的挑战，尤其是在小样本量中。在这里，我们提出了一种基于粘弹性的微流体系统，以连续，大小依赖且无标记的方式直接将外泌体从细胞培养基或血清中分离出来。在介质中使用少量生物相容性聚合物作为添加剂来控制施加在EV上的粘弹性力，我们就能实现外泌体的高分离纯度（> 90％）和回收率（> 80％）。

更新日期：2017-07-08

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