Immunomodulatory Activity of Ganoderma atrum Polysaccharide on Purified T Lymphocytes through Ca2+/CaN and Mitogen-Activated Protein Kinase Pathway Based on RNA Sequencing,Journal of Agricultural and Food Chemistry

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Immunomodulatory Activity of Ganoderma atrum Polysaccharide on Purified T Lymphocytes through Ca2+/CaN and Mitogen-Activated Protein Kinase Pathway Based on RNA Sequencing
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2017-06-26 00:00:00 , DOI: 10.1021/acs.jafc.7b01763
Quan-Dan Xiang ₁ , Qiang Yu ₁ , Hui Wang ₂ , Ming-Ming Zhao ₁ , Shi-Yu Liu ₁ , Shao-Ping Nie ₁ , Ming-Yong Xie ₁

Affiliation

Our previous study has demonstrated that Ganoderma atrum polysaccharide (PSG-1) has immunomodulatory activity on spleen lymphocytes. However, how PSG-1 exerts its effect on purified lymphocytes is still obscure. Thus, this study aimed to investigate the immunomodulatory activity of PSG-1 on purified T lymphocytes and further elucidate the underlying mechanism based on RNA sequencing (RNA-seq). Our results showed that PSG-1 promoted T lymphocytes proliferation and increased the production of IL-2, IFN-γ, and IL-12. Meanwhile, RNA-seq analysis found 394 differentially expressed genes. KEGG pathway analysis identified 20 significant canonical pathways and seven biological functions. Furthermore, PSG-1 elevated intracellular Ca²⁺ concentration and calcineurin (CaN) activity and raised the p-ERK, p-JNK, and p-p38 expression levels. T lymphocytes proliferation and the production of IL-2, IFN-γ, and IL-12 were decreased by the inhibitors of calcium channel and mitogen-activated protein kinases (MAPKs). These results indicated that PSG-1 possesses immunomodulatory activity on purified T lymphocytes, in which Ca²⁺/CaN and MAPK pathways play essential roles.

中文翻译：

基于RNA测序的Ca ²⁺ / CaN和丝裂原活化的蛋白激酶途径对灵芝多糖对纯化的T淋巴细胞的免疫调节活性。

我们以前的研究表明，灵芝多糖（PSG-1）对脾淋巴细胞具有免疫调节活性。但是，PSG-1如何对纯化的淋巴细胞发挥作用仍然不清楚。因此，本研究旨在研究PSG-1对纯化的T淋巴细胞的免疫调节活性，并进一步阐明基于RNA测序（RNA-seq）的潜在机制。我们的结果表明，PSG-1促进T淋巴细胞增殖并增加IL-2，IFN-γ和IL-12的产生。同时，RNA-seq分析发现了394个差异表达的基因。KEGG通路分析确定了20条重要的规范通路和7种生物学功能。此外，PSG-1升高了细胞内Ca ²⁺浓度和钙调神经磷酸酶（CaN）活性，并提高了p-ERK，p-JNK和p-p38的表达水平。钙通道和有丝分裂原激活的蛋白激酶（MAPK）的抑制剂可降低T淋巴细胞的增殖以及IL-2，IFN-γ和IL-12的产生。这些结果表明，PSG-1对纯化的T淋巴细胞具有免疫调节活性，其中Ca ²⁺ / CaN和MAPK途径发挥重要作用。

更新日期：2017-06-28

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