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Putative looping factor ZNF143/ZFP143 is an essential transcriptional regulator with no looping function
Molecular Cell ( IF 14.5 ) Pub Date : 2024-12-20 , DOI: 10.1016/j.molcel.2024.11.032 Domenic N. Narducci, Anders S. Hansen
Molecular Cell ( IF 14.5 ) Pub Date : 2024-12-20 , DOI: 10.1016/j.molcel.2024.11.032 Domenic N. Narducci, Anders S. Hansen
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Interactions between distal loci, including those involving enhancers and promoters, are a central mechanism of gene regulation in mammals, yet the protein regulators of these interactions remain largely undetermined. The zinc-finger transcription factor (TF) ZNF143/ZFP143 has been strongly implicated as a regulator of chromatin interactions, functioning either with or without CTCF. However, how ZNF143/ZFP143 functions as a looping factor is not well understood. Here, we tagged both CTCF and ZNF143/ZFP143 with dual-purpose degron/imaging tags to combinatorially assess their looping function and effect on each other. We find that ZNF143/ZFP143, contrary to prior reports, possesses no general looping function in mouse and human cells and that it largely functions independently of CTCF. Instead, ZNF143/ZFP143 is an essential and highly conserved transcription factor that largely binds promoters proximally, exhibits an extremely stable chromatin dwell time (>20 min), and regulates an important subset of mitochondrial and ribosomal genes.
更新日期：2024-12-20
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