BACKGROUNDThe overexpression of metabolic enzymes constitutes a crucial mechanism for insects to detoxify xenobiotics and metabolic pesticides. A flavin‐containing monooxygenase gene (PxFMO2) from Plutella xylostella and a P450 gene (SeCYP9A186, F116V mutant allele) from Spodoptera exigua have been reported to be involved in insecticide resistance. In this study, we aim to utilize transgenic technology to validate their in vivo detoxification functions in Plutella xylostella.RESULTSWe established two transgenic strains of Plutella xylostella with expressing an endogenous PxFMO2 gene from Plutella xylostella and an exogenous SeCYP9A186 gene from S. exigua, respectively. Bioassays demonstrated that the transgenic Plutella xylostella strain (IPP‐FMO2) expressing PxFMO2 exhibited a 12‐fold resistance to emamectin benzoate and a 6.4‐fold resistance to chlorantraniliprole compared to the background strain (IPP‐S). In contrast, the transgenic Plutella xylostella strain (IPP‐9A186) expressing SeCYP9A186 displayed a 235‐fold resistance to emamectin benzoate and a 115‐fold resistance to abamectin. Moreover, resistance to emamectin benzoate in the IPP‐9A186 strain of Plutella xylostella was inherited as an incompletely dominant trait and was genetically linked to the transgene locus.CONCLUSIONSOur results not only elucidated the in vivo contribution of the PxFMO2 and SeCYP9A186 to the insecticide resistance phenotype in Plutella xylostella, but also provided a genetic engineering toolkit to manipulate resistance pathways. These insights and methodologies could further aid in developing sustainable pest management strategies in Plutella xylostella. © 2024 Society of Chemical Industry.

中文翻译：

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SeCYP9A186 和 PxFMO2 的转基因表达赋予木霉菌对甲维菌素苯甲酸盐的抗性


背景代谢酶的过表达构成了昆虫解毒外源性物质和代谢杀虫剂的关键机制。据报道，来自小菜夜蛾的含黄素单加氧酶基因 （PxFMO2） 和来自甜菜夜蛾的 P450 基因 （SeCYP9A186， F116V 突变等位基因） 与杀虫剂耐药性有关。在这项研究中，我们旨在利用转基因技术来验证它们在 Plutella xylostella 中的体内解毒功能。结果我们建立了两个转基因小木头菇菌株，分别表达一个来自小木头菇的内源 PxFMO2 基因和一个来自小叶球菌的外源 SeCYP9A186 基因。生物测定表明，与背景菌株 （IPP-S） 相比，表达 PxFMO2 的转基因 Plutella xylostella 菌株 （IPP-FMO2） 对甲维菌素苯甲酸盐的耐药性是 12 倍，对氯虫苯甲酰胺的耐药性是 6.4 倍。相比之下，表达 SeCYP9A186 的转基因 Plutella xylostella 菌株 （IPP-9A186） 对甲维菌素苯甲酸盐的耐药性是 235 倍，对阿维菌素的耐药性是 115 倍。此外，Plutella xylostella 的 IPP-9A186 菌株对甲维菌素苯甲酸盐的抗性是作为不完全显性性状遗传的，并且在遗传上与转基因基因位点有关。结论研究结果不仅阐明了 PxFMO2 和 SeCYP9A186 对 Plutella xylostella 杀虫剂抗性表型的体内贡献，而且为操纵耐药途径提供了基因工程工具包。这些见解和方法可以进一步帮助制定 Plutella xylostella 的可持续害虫管理策略。© 2024 化工学会.