BACKGROUNDThe tomato russet mite (TRM), Aculops lycopersici, is a strictly herbivorous and economically significant pest that infests Solanaceae plants, but its host suitability varies, showing high performance on tomatoes. Although symbiotic bacteria have been suggested to play crucial roles in the host adaptation of herbivores, their effects on TRM remain unclear.RESULTSIn this study, using next generation high‐throughput sequencing of the bacterial 16S rRNA data, we identified the bacterial diversity and community composition of TRM feeding on tomato, eggplant, and chili. Our results show no significant difference in the bacterial community composition of TRM across three host plants. However, the relative density of Escherichia coli (TRM_Escherichia) showed 9.36‐fold higher on tomato than on eggplant and chili. These results align with the observed TRM performance among three host plants. When TRM_Escherichia was reduced using antibiotics, the treated TRM decreased the population density on tomato. However, when we transferred TRM from eggplant to tomato, the population density of TRM increased, coinciding with an increase of the TRM_Escherichia density. These results indicate that TRM_Escherichia may affect the host suitability of TRM. Our fluorescence in situ hybridization (FISH) results further showed that TRM_Escherichia is primarily distributed in the salivary glands. Metagenomic data results suggest that TRM_Escherichia functions in food digestion and energy metabolism.CONCLUSIONWe provided the first comprehensive analysis of TRM bacterial communities. Our findings demonstrate that the symbiotic bacterium TRM_Escherichia may play crucial roles in the suitability of TRM feeding on different Solanaceae hosts. © 2024 Society of Chemical Industry.

中文翻译：

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共生细菌在食草性螨虫宿主的适宜性中起着至关重要的作用


背景番茄赤褐色螨 （TRM），Aculops lycopersici，是一种严格的食草性害虫，具有重要的经济意义，会侵扰茄科植物，但其寄主适宜性各不相同，对西红柿表现出很高的性能。尽管共生细菌被认为在食草动物的宿主适应中起着至关重要的作用，但它们对 TRM 的影响仍不清楚。结果在这项研究中，使用细菌 16S rRNA 数据的下一代高通量测序，我们确定了以番茄、茄子和辣椒为食的 TRM 的细菌多样性和群落组成。我们的结果表明，三种寄主植物之间 TRM 的细菌群落组成没有显着差异。然而，番茄上的大肠杆菌 （TRM_Escherichia） 的相对密度比茄子和辣椒高 9.36 倍。这些结果与在三种寄主植物中观察到的 TRM 性能一致。当使用抗生素降低 TRM_Escherichia 时，处理后的 TRM 降低了番茄上的种群密度。然而，当我们将 TRM 从茄子转移到番茄时，TRM 的种群密度增加，同时TRM_Escherichia密度增加。这些结果表明，TRM_Escherichia可能会影响 TRM 的宿主适宜性。我们的荧光原位杂交 （FISH） 结果进一步表明，TRM_Escherichia主要分布在唾液腺中。宏基因组数据结果表明，TRM_Escherichia在食物消化和能量代谢中发挥作用。结论我们提供了 TRM 细菌群落的首次全面分析。我们的研究结果表明，共生细菌 TRM_Escherichia 可能在 TRM 以不同的茄科宿主为食的适用性中起关键作用。© 2024 化工学会.