BackgroundDiagnosis of allergies is mostly based on the patient's clinical history and allergen provocation tests. Determination of specific IgE (sIgE) profiles can be performed to support allergy diagnosis. This is commonly done in vivo by the skin prick test or in vitro with automated systems. Several platforms exist to quantify sIgE levels, but all these methods require access to specific instruments, often delaying the test's results. The IgE luciferase‐linked immunosorbent assay (LuLISA) allows bioluminescent quantification of IgE against peanut in microliter samples, but this method awaits extension to other allergens. This study aimed to validate a new method, named LuLIPLEX, for multiplexed bioluminescent detection of sIgE against 20 major molecular allergens.MethodsQuantification of sIgE against 12 recombinant or purified food allergens and eight aeroallergens was performed by LuLIPLEX versus standard IgE detection methods (ImmunoCAP, ISAC, ALEX, or NOVEOS). Multiplexed detection of IgE against these 20 allergens was performed within 45 min using 50 μL of serum, plasma, or whole blood samples.ResultsA head‐to‐head comparison between LuLIPLEX and standard IgE detection methods showed a high correlation among all allergens tested. sIgE profiles in polysensitized subjects could be determined within 45 min in serum and plasma samples, as well as using a single drop of capillary blood.ConclusionsLuLIPLEX is a rapid and sensitive method to quantify sIgE levels against multiple allergens. Given that the test is very fast and can be performed on small and inexpensive luminometers, the IgE LuLIPLEX could allow point‐of‐care testing of sIgE profiles in allergic subjects.

中文翻译：

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LuLIPLEX：一种快速、高灵敏度和多重检测主要过敏原 IgE 的方法


背景过敏的诊断主要基于患者的临床病史和过敏原激发试验。可以测定特异性 IgE （sIgE） 谱以支持过敏诊断。这通常通过皮肤点刺试验在体内完成，或使用自动化系统在体外完成。有几种平台可以量化 sIgE 水平，但所有这些方法都需要使用特定的仪器，通常会延迟检测结果。IgE 荧光素酶连接的免疫吸附测定 （LuLISA） 允许对微升样品中针对花生的 IgE 进行生物发光定量，但这种方法有待推广到其他过敏原。本研究旨在验证一种名为 LuLIPLEX 的新方法，该方法用于对 20 种主要分子过敏原进行 sIgE 的多重生物发光检测。方法使用 LuLIPLEX 与标准 IgE 检测方法 （ImmunoCAP、ISAC、ALEX 或 NOVEOS） 对 12 种重组或纯化食品过敏原和 8 种空气过敏原的 sIgE 进行定量。使用 50 μL 血清、血浆或全血样品在 45 分钟内对这 20 种过敏原的 IgE 进行多重检测。结果LuLIPLEX 与标准 IgE 检测方法之间的头对头比较显示，所有测试的过敏原之间都具有高度相关性。可以在 45 分钟内在血清和血浆样品中测定多致敏受试者的 sIgE 谱，也可以使用一滴毛细血管血。结论LuLIPLEX 是一种快速、灵敏的定量多种过敏原 sIgE 水平的方法。鉴于该测试非常快速，并且可以在小型且廉价的发光仪上进行，因此 IgE LuLIPLEX 可以对过敏受试者的 sIgE 谱进行即时测试。