Estrogen receptors (ER) are widely expressed in the brain of many species and experimental results highlighted the role of estradiol in neuronal plasticity and behavior. Consequently, the brain is therefore a prime target for endocrine disrupting chemicals (EDCs) interacting with estrogen signaling. Very little is known about the late effects of early disruption of estrogen signaling by EDCs. We focused on: ethinylestradiol (EE2; ER agonist) and clotrimazole (inhibitor of key steroidogenesis enzymes, including aromatases). Zebrafish eleutheroembryos were exposed (0–5 days) and then raised normally until adulthood. Several behavioral tests were performed in adults, then cell proliferation and dopaminergic neurons were quantified in several brain regions using immunostaining. Overall, a developmental exposure to EDCs stimulates cell proliferation in the dorsal telencephalon. At environmentally-relevant concentrations, male fish exposed to EE2 exhibited increased activity levels and decreased social behavior, posing a potential risk to population balance and health.

中文翻译：

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胚胎暴露于炔雌醇和克霉唑对斑马鱼行为和神经可塑性的长期影响 （Danio rerio）


雌激素受体 （ER） 在许多物种的大脑中广泛表达，实验结果突出了雌二醇在神经元可塑性和行为中的作用。因此，大脑是内分泌干扰化学物质 （EDC） 与雌激素信号相互作用的主要目标。关于 EDC 早期破坏雌激素信号传导的迟发效应知之甚少。我们专注于：炔雌醇 （EE2;ER 激动剂）和克霉唑（关键类固醇生成酶的抑制剂，包括芳香酶）。斑马鱼刺五加胚胎暴露（0-5 天），然后正常饲养直至成年。在成人中进行了几次行为测试，然后使用免疫染色量化了几个大脑区域的细胞增殖和多巴胺能神经元。总体而言，发育性暴露于 EDC 会刺激端脑背侧的细胞增殖。在环境相关浓度下，暴露于 EE2 的雄性鱼表现出活动水平增加和社会行为减少，对种群平衡和健康构成潜在风险。