The supercritical CO2 impregnation of pink pepper essential oil (EO) can bring its bioactive properties to a biomaterial in a green approach, making it multifunctional. Aiming to obtain a gelatin-siloxane cryogel loaded with pink pepper EO for biomedical applications and to understand the impact of the impregnation conditions, gelatin was crosslinked with (3-glycidoxypropyl)trimethoxysilane and lyophilized, and the impregnation was studied using a design of experiments with conditions from 10 to 30 MPa and 35–60 °C. The cryogel synthesis was confirmed by FTIR, %gel and TGA. Its biocompatibility was demonstrated through SEM, water uptake and cytotoxicity studies. It was shown that, due to the influence on the solubility of the EO in scCO2, varying pressure and temperature influences the impregnation, which showed {20 MPa; 35 °C} as its best condition. Therefore, a cryogel suitable for implantation with potential to reduce side effects related with infection and inflammation was obtained.

中文翻译：

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粉红胡椒精油在明胶-硅氧烷低温凝胶中的超临界 CO2 浸渍，用于生物医学应用


粉红胡椒精油 （EO） 的超临界 CO2 浸渍可以以绿色方式将其生物活性特性带到生物材料中，使其具有多功能性。为了获得用于生物医学应用的载有粉红胡椒 EO 的明胶-硅氧烷冷冻凝胶并了解浸渍条件的影响，将明胶与（3-缩水甘油氧丙基）三甲氧基硅烷交联并冻干，并使用 10 至 30 MPa 和 35-60 °C 条件下的实验设计研究浸渍。 通过 FTIR 、 %gel 和 TGA 确认冷冻凝胶合成。其生物相容性通过 SEM、水分吸收和细胞毒性研究得到证实。结果表明，由于对 EO 在 scCO2 中的溶解度的影响，不同的压力和温度会影响浸渍，这表明 {20 MPa;35 °C} 是其最佳条件。因此，获得了一种适合植入的冷冻凝胶，有可能减少与感染和炎症相关的副作用。