ScopeCordyceps cicadae polysaccharides have received attention due to their potential in treating hyperglycemia and enhancing renal function. The beneficial effect of the purified C. cicadae polysaccharides fraction (CCP‐1) on the reproductive impairments and spermatogenesis dysfunction of immunocompromised mice is unavailable and is studied herein.Methods and resultsThe study establishes a GC‐1 spg cell apoptosis model induced by TNF‐α+SM‐164 (TS) and male mouse reproductive injury model induced by cyclophosphamide (CTX), and then intervened by CCP‐1. CCP‐1 improves the viability of GC‐1 spg cell and inhibits cells apoptosis induced by TS in vitro. CCP‐1 enhances sperm quality and spermatogenesis function, as well as ameliorating the histological lesions in the hypothalamus, testicular, and kidney. CCP‐1 elevates gonadotropin‐releasing hormone (GnRH) level that secreted by the hypothalamus, and increases the levels of follicle stimulating hormone (FSH) and luteizing hormone (LH) in the anterior pituitary stimulated by GnRH, and promotes the secretion of testosterone (T) by testis. Moreover, CCP‐1 could protect the reproductive system by activating reproductive regulatory pathway such as SCF/C‐kit pathway and inhibiting apoptotic signaling pathway such as Bax/Caspase‐3 pathway.ConclusionThese results manifest that CCP‐1 could serve as a natural promising reproductive system protective supplement for ameliorating CTX biotoxicity.

中文翻译：

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冬虫夏草中的多糖通过下丘脑-垂体-睾丸轴改善雄性小鼠的生殖障碍


范围冬虫夏草蝉多糖因其在治疗高血糖和增强肾功能方面的潜力而受到关注。纯化的 C. cicadae 多糖组分 （CCP-1） 对免疫功能低下小鼠的生殖障碍和精子发生功能障碍的有益作用尚不清楚，在本文中进行研究。方法和结果该研究建立了 TNF-α+SM-164 （TS） 诱导的 GC-1 spg 细胞凋亡模型和环磷酰胺 （CTX） 诱导的雄性小鼠生殖损伤模型，然后由 CCP-1 干预。CCP-1 在体外提高 GC-1 spg 细胞的活力并抑制 TS 诱导的细胞凋亡。CCP-1 增强精子质量和精子发生功能，并改善下丘脑、睾丸和肾脏的组织学病变。CCP-1 提高下丘脑分泌的促性腺激素释放激素 （GnRH） 水平，增加 GnRH 刺激的垂体前叶促卵泡激素 （FSH） 和黄体生成素 （LH） 水平，促进睾丸分泌睾酮 （T）。此外，CCP-1 可以通过激活生殖调节途径（如 SCF/C-kit 通路）和抑制凋亡信号通路（如 Bax/Caspase-3 通路）来保护生殖系统。结论这些结果表明，CCP-1 可以作为一种天然有前途的生殖系统保护补充剂，用于改善 CTX 的生物毒性。