Purpose: Ingestion of whey protein increases myofibrillar but not muscle connective protein synthesis rates. Recently, we defined a whey and collagen protein blend (5:1-ratio) to optimize post-prandial plasma amino acid availability. Here, we assessed the ability of this blend to increase myofibrillar and muscle connective protein synthesis rates at rest and during early recovery from exercise.Methods: In a randomized, double-blind, parallel design, 28 men (age: 25 ± 5 y; BMI: 23.6 ± 2.3 kg/m2) were randomly allocated to ingest either 30 g of protein (25 g whey/5 g collagen; BLEND, n = 14) or a non-caloric placebo (PLA, n = 14) following a single session of unilateral leg resistance-type exercise. Participants received primed continuous L-[ring-13C6]-phenylalanine infusions with blood and muscle biopsy samples collection for 5 hours post-prandially to assess myofibrillar and muscle connective protein synthesis rates.Results: Protein ingestion strongly increased plasma amino acid concentrations, including plasma leucine and glycine concentrations (P < 0.001), with no changes following placebo ingestion (P > 0.05). Post-prandial myofibrillar and muscle connective protein synthesis rates were higher in the exercised compared to the rested leg (P < 0.001). In addition, myofibrillar protein synthesis rates were higher in BLEND compared to PLA in both the rested (0.038 ± 0.008 and 0.031 ± 0.006%·h-1, respectively; P < 0.05) and exercised (0.052 ± 0.011 and 0.039 ± 0.009%·h-1, respectively; P < 0.01) leg. Muscle connective protein synthesis rates were higher in BLEND compared to PLA in the rested (0.062 ± 0.013 and 0.051 ± 0.010%·h-1, respectively; P < 0.05), but not the exercised (0.090 ± 0.021 and 0.079 ± 0.016%·h-1, respectively; P = 0.11) leg.Conclusions: Ingestion of a whey (25 g) plus collagen (5 g) protein blend increases both myofibrillar and muscle connective protein synthesis rates at rest and further increases myofibrillar but not muscle connective protein synthesis rates during recovery from exercise in recreationally active, young men.

中文翻译：

---

摄入乳清加胶原蛋白混合物可增加肌原纤维和肌肉结缔蛋白的合成速率。


目的：摄入乳清蛋白会增加肌原纤维的合成率，但不会增加肌肉结缔蛋白的合成率。最近，我们定义了一种乳清和胶原蛋白混合物（5：1 比例），以优化餐后血浆氨基酸的可用性。在这里，我们评估了这种混合物在休息和运动早期恢复期间增加肌原纤维和肌肉结缔蛋白合成率的能力。方法：在随机、双盲、平行设计中，28 名男性 （年龄：25 ± 5 岁;BMI：23.6 ± 2.3 kg/m2）随机分配摄入 30 g 蛋白质（25 g 乳清/5 g 胶原蛋白;BLEND，n = 14）或无热量安慰剂（PLA，n = 14）在单次单侧腿部阻力型运动后。参与者在餐后 5 小时接受初免连续 L-[ring-13C6]-苯丙氨酸输注和血液和肌肉活检样本采集，以评估肌原纤维和肌肉结缔蛋白合成率。结果： 蛋白质摄入强烈增加了血浆氨基酸浓度，包括血浆亮氨酸和甘氨酸浓度 （P < 0.001），摄入安慰剂后没有变化 （P > 0.05）。与休息的腿相比，运动中的餐后肌原纤维和肌肉结缔蛋白合成率更高 （P < 0.001）。此外，与PLA相比，BLEND中的肌原纤维蛋白合成速率在静置组中均较高（分别为0.038±0.008和0.031±0.006%·h-1;P < 0.05） 和运动 （0.052 ± 0.011 和 0.039 ± 0.009%·h-1;P < 0.01） 腿。与静息组的 PLA 相比，BLEND 的肌肉结缔蛋白合成率更高 （分别为 0.062 ± 0.013 和 0.051 ± 0.010%·h-1;P < 0.05），但未行使 （分别为 0.090 ± 0.021 和 0.079 ± 0.016%·h-1;P = 0.11） 腿。结论：摄入乳清 （25 g） 加胶原蛋白 （5 g） 蛋白质混合物可增加静息时肌原纤维和肌肉结缔蛋白的合成率，并进一步增加肌原纤维而不是肌肉结缔蛋白的合成率，在娱乐活跃的年轻男性从运动中恢复期间。