Delirium is a multifactorial medical condition characterized by impairment across various mental functions and is one of the greatest risk factors for prolonged hospitalization, morbidity, and mortality. Research focused on delirium has proven to be challenging due to a lack of objective measures for diagnosing patients, and few laboratory models have been validated. Our recent studies report the efficacy of bispectral electroencephalography (BSEEG) in diagnosing delirium in patients and predicting patient outcomes. We applied BSEEG to validate a lipopolysaccharide (LPS)-induced mouse model of delirium. Moreover, we investigated the relationship between BSEEG score, delirium-like behaviors, and microglia activation in hippocampal dentate gyrus and cortex regions in young and aged mice. There was a significant correlation between BSEEG score and impairment of attention in young mice. Additionally, there was a significant correlation between BSEEG score and microglial activation in hippocampal dentate gyrus and cortex regions in young and aged mice. We have successfully validated the BSEEG method by showing its associations with a level of behavioral change and microglial activation in an LPS-induced mouse model of delirium. In addition, the BSEEG method was able to sensitively capture an LPS-induced delirium-like condition that behavioral tests could not capture because of a hypoactive state.

中文翻译：

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LPS 诱导的小鼠谵妄样行为和小胶质细胞激活与双频脑电图 （BSEEG） 相关


谵妄是一种多因素疾病，其特征是各种心理功能受损，是长期住院、发病和死亡的最大风险因素之一。由于缺乏诊断患者的客观措施，专注于谵妄的研究已被证明具有挑战性，并且很少有实验室模型得到验证。我们最近的研究报告了双频脑电图 （BSEEG） 在诊断患者谵妄和预测患者预后方面的疗效。我们应用 BSEEG 来验证脂多糖 （LPS） 诱导的谵妄小鼠模型。此外，我们研究了年轻和老年小鼠海马齿状回和皮层区域 BSEEG 评分、谵妄样行为和小胶质细胞激活之间的关系。BSEEG 评分与年轻小鼠注意力障碍之间存在显着相关性。此外，BSEEG 评分与年轻和老年小鼠海马齿状回和皮层区域的小胶质细胞激活之间存在显着相关性。我们通过在 LPS 诱导的谵妄小鼠模型中显示其与行为改变水平和小胶质细胞激活的关联，成功地验证了 BSEEG 方法。此外，BSEEG 方法能够灵敏地捕捉到 LPS 诱导的谵妄样病症，而行为测试由于活动减退状态而无法捕捉到这种病症。