Advanced optical imaging provides a powerful tool for the structural and functional analysis of tissues with high resolution and contrast, but the imaging performance decreases as light propagates deeper into the tissue. Tissue optical clearing technique demonstrates an innovative way to realize deep-tissue imaging and have emerged substantially in the last two decades. Here, we briefly reviewed the basic principles of tissue optical clearing techniques in the view of delivery strategies via either free diffusion or external forces-driven advection, and the commonly-used optical techniques for monitoring kinetics of clearing agents in tissue, as well as their ex vivo to in vivo applications in multiple biomedical research fields. With future efforts on the even distribution of both clearing agents and probes, excavation of more effective clearing agents, and automation of tissue clearing processes, tissue optical clearing should provide more insights into the fundamental questions in biological events clinical diagnostics.

中文翻译：

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浸没式光学透明化剂在组织中的递送和动力学：从离体到体内的光学成像


先进的光学成像技术为组织结构和功能分析提供了强大的工具，具有高分辨率和对比度，但随着光深入组织，成像性能会下降。组织光学透明化技术展示了一种实现深层组织成像的创新方法，并在过去二十年中大量出现。在这里，我们从通过自由扩散或外力驱动平流的递送策略的角度简要回顾了组织光学透明化技术的基本原理，以及用于监测组织中透明化剂动力学的常用光学技术，以及它们在多个生物医学研究领域的离体到体内应用。随着未来在透明化剂和探针的均匀分布、更有效的透明化剂的挖掘以及组织透明化过程的自动化方面的努力，组织光学透明化应该为生物事件临床诊断中的基本问题提供更多见解。