Okara protein (OP) is a potential plant-based protein that is beneficial to human health. In this work, an alternating ultrasonic/alkali treatment method with non-continued cavitation and thermal energy output was used to extract protein (AUA-OP) from okara to enhance the functional properties of OP and improve the stability of OP-based emulsions. The purity of AUA-OP was greater than 80%. Compared with traditional (physical-assisted) alkali treatment, FTIR and SDS-PAGE revealed that AUA-OP retained the chemical structure of the protein, but the number of ultrasound-induced exposure sites increased, with increased fluorescence intensity, surface hydrophobicity, and absolute ζ-potential. After alternating ultrasonic/alkali treatment, the protein particles were looser and smaller. In addition, the water/oil holding capacity, EAI, and ESI of AUA-OP further increased. The viscosity of the AUA-OP-stabilized emulsion was also greater. Finally, a 28-day emulsion storage assay revealed that the AUA-OP-stabilized emulsion was stable with a relatively low droplet size and creaming index, indicating great potential for the development of stable protein-based emulsions.

中文翻译：

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超声/碱交替处理提取的豆渣蛋白及其改进的理化和功能特性


豆渣蛋白 （OP） 是一种对人体健康有益的潜在植物蛋白。在本工作中，采用非连续空化和热能输出的超声波/碱交替处理方法从豆渣中提取蛋白质 （AUA-OP），以增强 OP 的功能特性并提高 OP 基乳液的稳定性。AUA-OP 的纯度大于 80%。与传统的 （物理辅助） 碱处理相比，FTIR 和 SDS-PAGE 显示 AUA-OP 保留了蛋白质的化学结构，但超声诱导的暴露部位数量增加，荧光强度、表面疏水性和绝对ζ电位增加。超声/碱交替处理后，蛋白质颗粒更松散、更小。此外，AUA-OP 的水/油持水量、EAI 和 ESI 进一步增加。AUA-OP 稳定乳液的粘度也更大。最后，28 天的乳剂储存测定表明，AUA-OP 稳定的乳剂是稳定的，液滴大小和乳化指数相对较低，表明开发稳定的蛋白质基乳剂具有很大的潜力。