An ultralow cathodic potential electrochemiluminescence (ECL) aptasensor was designed, employing DNA nanoribbon template self-assembly copper nanoclusters (DNR-CuNCs) as a novel coreaction accelerator within the luminol-H₂O₂ system for the sensitive detection of kanamycin (KANA). Mechanistic investigations revealed that the DNR-CuNCs preferred to generate highly active hydroxyl radicals by facilitating the reduction of the coreactant H₂O₂ under neutral pH conditions, consequently enhancing cathodic luminescence. By the strong π–π stacking effect of KANA aptamer and graphene as a signal modulation switch, DNR-CuNCs were displaced from the electrode surface due to the affinity of KANA and its aptamer, resulting in the inhibition of the luminol-H₂O₂ system and a decrease in the ECL signal. Under optimal experiments, the aptasensor demonstrated exceptional sensitivity in detecting KANA within the concentration range from 1 × 10^–2 to 5 × 10⁵ pg/mL, with the detection limit as low as 0.18 fg/mL. This innovative strategy provided a novel approach to designing effective ECL emitters for monitoring food safety.

中文翻译：

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使用铜纳米带作为共反应促进剂检测卡那霉素的超低电位阴极电化学发光 Aptasensor


设计了一种超低阴极电位电化学发光 （ECL） 适配传感器，采用 DNA 纳米带模板自组装铜纳米簇 （DNR-CuNCs） 作为鲁米诺-H₂O₂ 系统内的新型共反应促进剂，用于灵敏检测卡那霉素 （KANA）。机理研究表明，DNR-CuNCs 在中性 pH 条件下通过促进共反应物 H₂O₂ 的还原来产生高活性羟基自由基，从而增强阴极发光。利用 KANA 适配体和石墨烯作为信号调制开关的强π-π堆叠效应，由于 KANA 及其适配体的亲和力，DNR-CuNCs 从电极表面移位，导致鲁米诺-H₂O₂ 系统受到抑制，ECL 信号减弱。在最佳实验下，aptasensor 在 1 × ^10-2 至 5 × 10⁵ pg/mL 的浓度范围内检测 KANA 时表现出卓越的灵敏度，检测限低至 0.18 fg/mL。这种创新策略为设计有效的 ECL 排放源以监测食品安全提供了一种新方法。