Using two kinds of microbial inoculations extracted from soil cropped to rice and peanut, we conducted a swap-inoculation experiment to explore the relative importance of microbial inoculation and soil properties on CO₂ emissions from soil. Inoculated microorganisms into a soil different from their origin (swap inoculation) were partially successful and reduced CO₂ emissions, namely according to home-field advantage (HFA); The success of invasive microorganisms depended on molecular composition of soil organic matter (SOM) compared to inoculation of native microbes (inoculated microorganisms into origin soil). The different habits screened the fewer microorganisms to undergo respiration for energy and life-sustaining activities, thus decreasing CO₂ emissions from SOM. However, the effect of HFA diminished with incubation time, as the invasive microorganisms reshaped SOM molecular diversity and composition during microbial community assemblage, which fits with the Gaia effect (GE). Specific microbial communities, such as Bacteroidetes and Actinobacteria, drove the conversion of persistent molecules to labile molecules, thereby increasing the chances of SOM mineralization by microorganisms. We found there was positive correlation between labile SOM molecules and SOM mineralization. In addition, MBC increased in swap inoculation compared to native inoculation after 60 days, which also resulted in higher CO₂ emissions from SOM. HFA and GE provide new perspectives to help decipher the interaction between microorganisms and the habitat under microbial invasion, and the mechanism of influence on CO₂ emissions from SOM.

使用从水稻和花生种植的土壤中提取的两种微生物接种物，我们进行了交换接种实验，以探讨微生物接种和土壤特性对土壤 CO₂ 排放的相对重要性。将微生物接种到与其来源不同的土壤中（交换接种）部分成功，并减少了 CO₂ 排放，即根据主场优势 （HFA）;与天然微生物（接种到原产地土壤中的微生物）相比，入侵微生物的成功取决于土壤有机质 （SOM） 的分子组成。不同的习性筛选了较少的微生物进行呼吸以获得能量和维持生命的活动，从而减少了 SOM 的_CO2 排放。然而，HFA 的影响随着孵育时间的延长而减弱，因为入侵微生物在微生物群落组装过程中重塑了 SOM 分子多样性和组成，这与盖亚效应 （GE） 相吻合。特定的微生物群落，如拟杆菌门和放线菌门，驱动持久性分子转化为不稳定分子，从而增加了微生物 SOM 矿化的机会。我们发现不稳定的 SOM 分子与 SOM 矿化之间存在正相关。此外，与天然接种相比，60 天后交换接种的 MBC 增加，这也导致 SOM 的 CO₂ 排放量增加。HFA 和 GE 提供了新的视角，有助于破译微生物与微生物入侵下栖息地之间的相互作用，以及 SOM 对 COM_{排放的影响}机制。