Drought stress is a serious natural challenge for tea plants that significantly affects tea yield and quality. miR171s play critical roles in plant stress responses, however, their role in drought stress tolerance in tea plants () is poorly understood. This study experimentally verified the expression patterns of csn-miR171b-3p_2 and its target, (). We found that csn-miR171b-3p_2 could target and regulate to play an important role in the defense against drought stress in tea plants. CsSCL6-4 is located in the nucleus and is self-activated in . In addition, we obtained 819 putative binding regions of CsSCL6-4 using DNA affinity purification sequencing analysis, which were assigned to 786 different genes, four of which were drought-resistant genes (, , , and ). Yeast one-hybrid and dual-luciferase reporter assays revealed that CsSCL6-4 directly promoted the expression of these four drought resistance genes by binding motifs 1/2/3 in their promoter regions. Both overexpression and suppression of proved that participated in the defense against drought stress in tea plants by regulating the expression of , , , and . In addition, suppression of csn-miR171b-3p_2 expression significantly increased the expression of and activated -bound gene transcription under drought stress. Therefore, the csn-miR171b-3p_2-CsSCL6-4 module participates in tea plant resistance to drought stress by promoting the expression of drought resistance genes. Our results revealed the function of csn-miR171b-3p_2 in tea plants and provided new insights into the mechanism of tea plant resistance to drought stress.

中文翻译：

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csn-miR171b-3p_2靶向CsSCL6-4参与茶树抵抗干旱胁迫


干旱胁迫是茶树面临的严峻自然挑战，显着影响茶叶产量和品质。 miR171 在植物逆境响应中发挥着关键作用，然而，它们在茶树干旱胁迫耐受性中的作用却知之甚少。本研究通过实验验证了csn-miR171b-3p_2及其靶标()的表达模式。我们发现csn-miR171b-3p_2可以靶向和调节，在茶树防御干旱胁迫中发挥重要作用。 CsSCL6-4 位于细胞核中并在 中自我激活。此外，我们通过DNA亲和纯化测序分析获得了819个CsSCL6-4的假定结合区域，这些区域被分配给786个不同的基因，其中四个是抗旱基因（、、、和）。酵母单杂交和双荧光素酶报告基因分析表明，CsSCL6-4 通过结合启动子区域的基序 1/2/3 直接促进这四种抗旱基因的表达。证明 的过表达和抑制均通过调节 、 、 和 的表达参与茶树抵御干旱胁迫的过程。此外，在干旱胁迫下，csn-miR171b-3p_2表达的抑制显着增加了结合基因的表达并激活了结合基因的转录。因此，csn-miR171b-3p_2-CsSCL6-4模块通过促进抗旱基因的表达参与茶树对干旱胁迫的抵抗。我们的研究结果揭示了csn-miR171b-3p_2在茶树中的功能，并为茶树抵抗干旱胁迫的机制提供了新的见解。